Rabbit Recombinant Monoclonal Fibroblast activation protein, alpha antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FAP aa 1 to C-terminus.
IgG
Rabbit
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Liquid
Monoclonal
ICC | IHC-P | IP | WB | |
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Human | Tested | Tested | Tested | Tested |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2 (PubMed:14751930, PubMed:16223769, PubMed:16410248, PubMed:16480718, PubMed:17381073, PubMed:18095711, PubMed:21288888, PubMed:24371721). Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vitronectin, tenascin, laminin, fibronectin, fibrin or casein (PubMed:10347120, PubMed:10455171, PubMed:12376466, PubMed:16223769, PubMed:16651416, PubMed:18095711, PubMed:2172980, PubMed:7923219, PubMed:9065413). Also has dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro (PubMed:10347120, PubMed:10593948, PubMed:16175601, PubMed:16223769, PubMed:16410248, PubMed:16651416, PubMed:17381073, PubMed:21314817, PubMed:24371721, PubMed:24717288). Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB) (PubMed:21314817). The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.
Prolyl endopeptidase FAP, 170 kDa melanoma membrane-bound gelatinase, Dipeptidyl peptidase FAP, Fibroblast activation protein alpha, Gelatine degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme, Serine integral membrane protease, Surface-expressed protease, FAPalpha, SIMP, Seprase, FAP
Rabbit Recombinant Monoclonal Fibroblast activation protein, alpha antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FAP aa 1 to C-terminus.
IgG
Rabbit
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Liquid
Monoclonal
BLR150J
Blue Ice
Up to 12 months
+4°C
+4°C
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Detection of human Fibroblast Activation Protein alpha/FAP FFPE human breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-Fibroblanst Activation Protein alpha/FAP recombinant monoclonal antibody [BLR150J] (A700-150). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Detection of human Fibroblast Activation Protein alpha/FAP in FFPE human BJ cells by immunocytochemistry. Antibody: Rabbit anti-Fibroblanst Activation Protein alpha/FAP recombinant monoclonal antibody [BLR150J] (A700-150). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Samples: Whole cell lysate (50 µg) from SNB-75, HeLa, IMR-90, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FAP recombinant monoclonal antibody [BLR150J] (A700-150) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.
All lanes: Western blot - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (ab314075) at 1/1000 dilution
Lane 1: SNB-75 Whole cell lysate at 50 µg
Lane 2: HeLa Whole cell lysate at 50 µg
Lane 3: IMR-90 Whole cell lysate at 50 µg
Lane 4: HEK293T Whole cell lysate at 50 µg
Lane 5: U2OS Whole cell lysate at 50 µg
All lanes: HRP-conjugated goat anti-rabbit IgG
Samples: Whole cell lysate (500 µg per IP reaction; 5% of IP loaded) from IMR-90 cells. Antibody: Rabbit anti-FAP recombinant monoclonal antibody [BLR150J] (A700-150)used for IP at 20 µl/mg lysate. For blotting immunoprecipitated FAP, A700-150 was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.
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