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Rabbit Recombinant Monoclonal Fibroblast activation protein, alpha antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FAP aa 1 to C-terminus.

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Images

Immunohistochemistry - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (AB314075), expandable thumbnail
  • Immunocytochemistry - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (AB314075), expandable thumbnail
  • Western blot - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (AB314075), expandable thumbnail
  • Immunoprecipitation - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (AB314075), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human FAP aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q12884

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCIHC-PIPWB
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2 (PubMed:14751930, PubMed:16223769, PubMed:16410248, PubMed:16480718, PubMed:17381073, PubMed:18095711, PubMed:21288888, PubMed:24371721). Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vitronectin, tenascin, laminin, fibronectin, fibrin or casein (PubMed:10347120, PubMed:10455171, PubMed:12376466, PubMed:16223769, PubMed:16651416, PubMed:18095711, PubMed:2172980, PubMed:7923219, PubMed:9065413). Also has dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro (PubMed:10347120, PubMed:10593948, PubMed:16175601, PubMed:16223769, PubMed:16410248, PubMed:16651416, PubMed:17381073, PubMed:21314817, PubMed:24371721, PubMed:24717288). Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB) (PubMed:21314817). The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Fibroblast activation protein, alpha antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FAP aa 1 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human FAP aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q12884
Clone number

BLR150J

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (ab314075), expandable thumbnail

    Immunohistochemistry - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (ab314075)

    Detection of human Fibroblast Activation Protein alpha/FAP FFPE human breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-Fibroblanst Activation Protein alpha/FAP recombinant monoclonal antibody [BLR150J] (A700-150). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Immunocytochemistry - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (ab314075), expandable thumbnail

    Immunocytochemistry - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (ab314075)

    Detection of human Fibroblast Activation Protein alpha/FAP in FFPE human BJ cells by immunocytochemistry. Antibody: Rabbit anti-Fibroblanst Activation Protein alpha/FAP recombinant monoclonal antibody [BLR150J] (A700-150). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Western blot - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (ab314075), expandable thumbnail

    Western blot - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (ab314075)

    Samples: Whole cell lysate (50 µg) from SNB-75, HeLa, IMR-90, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FAP recombinant monoclonal antibody [BLR150J] (A700-150) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

    All lanes: Western blot - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (ab314075) at 1/1000 dilution

    Lane 1: SNB-75 Whole cell lysate at 50 µg

    Lane 2: HeLa Whole cell lysate at 50 µg

    Lane 3: IMR-90 Whole cell lysate at 50 µg

    Lane 4: HEK293T Whole cell lysate at 50 µg

    Lane 5: U2OS Whole cell lysate at 50 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

  • Immunoprecipitation - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (ab314075), expandable thumbnail

    Immunoprecipitation - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (ab314075)

    Samples: Whole cell lysate (500 µg per IP reaction; 5% of IP loaded) from IMR-90 cells. Antibody: Rabbit anti-FAP recombinant monoclonal antibody [BLR150J] (A700-150)used for IP at 20 µl/mg lysate. For blotting immunoprecipitated FAP, A700-150 was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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