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AB314075

Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free

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(1 Publication)

Rabbit Recombinant Monoclonal Fibroblast activation protein, alpha antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FAP aa 1 to C-terminus.

View Alternative Names

Prolyl endopeptidase FAP, 170 kDa melanoma membrane-bound gelatinase, Dipeptidyl peptidase FAP, Fibroblast activation protein alpha, Gelatine degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme, Serine integral membrane protease, Surface-expressed protease, FAPalpha, SIMP, Seprase, FAP

4 Images
Immunocytochemistry - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (AB314075)
  • ICC

Supplier Data

Immunocytochemistry - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (AB314075)

Detection of human Fibroblast Activation Protein alpha/FAP in FFPE human BJ cells by immunocytochemistry. Antibody : Rabbit anti-Fibroblanst Activation Protein alpha/FAP recombinant monoclonal antibody [BLR150J] (A700-150). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Immunohistochemistry - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (AB314075)
  • IHC

Supplier Data

Immunohistochemistry - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (AB314075)

Detection of human Fibroblast Activation Protein alpha/FAP FFPE human breast carcinoma by immunohistochemistry. Antibody : Rabbit anti-Fibroblanst Activation Protein alpha/FAP recombinant monoclonal antibody [BLR150J] (A700-150). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Immunoprecipitation - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (AB314075)
  • IP

Supplier Data

Immunoprecipitation - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (AB314075)

Samples : Whole cell lysate (500 µg per IP reaction; 5% of IP loaded) from IMR-90 cells. Antibody : Rabbit anti-FAP recombinant monoclonal antibody [BLR150J] (A700-150)used for IP at 20 µl/mg lysate. For blotting immunoprecipitated FAP, A700-150 was used at 1 : 1000. Chemiluminescence with an exposure time of 30 seconds.

false

Western blot - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (AB314075)
  • WB

Supplier Data

Western blot - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (AB314075)

Samples : Whole cell lysate (50 µg) from SNB-75, HeLa, IMR-90, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody : Rabbit anti-FAP recombinant monoclonal antibody [BLR150J] (A700-150) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel : Rabbit anti-COPB2 antibody.

All lanes:

Western blot - Anti-Fibroblast Activation Protein alpha/FAP antibody [BLR150J] - BSA free (ab314075) at 1/1000 dilution

Lane 1:

SNB-75 Whole cell lysate at 50 µg

Lane 2:

HeLa Whole cell lysate at 50 µg

Lane 3:

IMR-90 Whole cell lysate at 50 µg

Lane 4:

HEK293T Whole cell lysate at 50 µg

Lane 5:

U2OS Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR150J

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC, WB, IP, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FAP aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q12884

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the FAP gene is a cell surface glycoprotein serine protease involved in the degradation of the extracellular matrix (ECM) and various cellular processes such as tissue remodeling, fibrosis, wound healing, inflammation, and tumor growth. It exists in both plasma membrane-bound and soluble forms, exhibiting post-proline cleaving endopeptidase activity, specifically targeting Ala/Ser-Gly-Pro-Ser/Asn/Ala sequences in substrates like alpha-2-antiplasmin SERPINF2 and SPRY2. FAP protein degrades gelatin and heat-denatured type I collagen but not native type I and IV collagens, nor vitronectin, tenascin, laminin, fibronectin, fibrin, or casein. It also has dipeptidyl peptidase activity, hydrolyzing prolyl bonds when the penultimate residue is proline, with a preference for sequences such as Ala-Pro and Gly-Pro. It acts on natural neuropeptides like neuropeptide Y, peptide YY, substance P, and brain natriuretic peptide 32. In its membrane form, in association with DPP4, PLAUR, or integrins, FAP is involved in pericellular ECM proteolysis, promoting cell adhesion, migration, invasion, and plays roles in development, wound healing, and cell invasiveness in malignant melanoma. It supports tumor progression by facilitating angiogenesis, collagen degradation, apoptosis, and reducing immune response. Additionally, it promotes glioma cell invasion by degrading brevican and functions as a tumor suppressor in melanocytic cells by regulating cell proliferation and survival independently of its serine protease activity. This supplementary information is collated from multiple sources and compiled automatically.
See full target information FAP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2411152 PubMed39950910

2025

Fibroblast Activation Protein Acts as a Biomarker for Monitoring ECM Remodeling During Aortic Aneurysm via Ga-FAPI-04 PET Imaging.

Applications

WB, WB

Species

Rabbit, Mouse

Chengkai Hu,Hui Tan,Yuchong Zhang,Genmao Cao,Chenye Wu,Peng Lin,Shouji Qiu,Fandi Mo,Enci Wang,Shiyi Li,Tong Yuan,Zheyun Li,Weiguo Fu,Dengfeng Cheng,Hao Lai,Xiaoyuan Chen,Lixin Wang
View all publications
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Product promise

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