Anti-Fibromodulin antibody [EPR23029-174]

• WB

Supplier Data

Western blot - Anti-Fibromodulin antibody [EPR23029-174] (AB267465)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 12623288, 8895216)

Negative control : Hut-78 (PMID : 15741214)

Exposure time : Lane 1 second; Lanes 2-6 : 3 seconds

All lanes:

Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465) at 1/1000 dilution

Lane 1:

Human articular cartilage lysate at 20 µg

Lane 2:

Human uterus lysate at 20 µg

Lane 3:

Human skin lysate at 20 µg

Lane 4:

HuT-78 (human sezary syndrome cutaneous t lymphocyte) whole cell lysate at 20 µg

Lane 5:

Mouse articular cartilage lysate at 20 µg

Lane 6:

Rat articular cartilage lysate at 20 µg

Secondary

Lanes 1 - 4:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Lanes 5 - 6:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 60-100 kDa

false

• WB

Lab

Western blot - Anti-Fibromodulin antibody [EPR23029-174] (AB267465)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 12623288, 8895216).

Two bands of 46kDa and 40kDa are de-glycosylated forms. The bands of 60-100kDa are glycosylated forms.

Exposure time : 3 minutes

All lanes:

Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465) at 1/1000 dilution

Lane 1:

Human uterus tissue lysate at 20 µg

Lane 2:

Human uterus tissue treated with PNGase F lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 60-100 kDa

false

• WB

Lab

Western blot - Anti-Fibromodulin antibody [EPR23029-174] (AB267465)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 114 seconds

All lanes:

Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465) at 1/1000 dilution

All lanes:

Recombinant Human Fibromodulin/FMOD protein (aa75-376) 20ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 43 kDa

Observed band size: 35 kDa

false