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Rabbit Recombinant Monoclonal Fibromodulin antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Western blot - Anti-Fibromodulin antibody [EPR23029-174] (AB267465), expandable thumbnail
  • Western blot - Anti-Fibromodulin antibody [EPR23029-174] (AB267465), expandable thumbnail
  • Western blot - Anti-Fibromodulin antibody [EPR23029-174] (AB267465), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Not recommended
Tested
Not recommended
Mouse
Not recommended
Tested
Not recommended
Rat
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Affects the rate of fibrils formation. May have a primary role in collagen fibrillogenesis (By similarity).

Alternative names

FM, SLRR2E, FMOD, Fibromodulin, Collagen-binding 59 kDa protein, Keratan sulfate proteoglycan fibromodulin, KSPG fibromodulin

Recommended products

Rabbit Recombinant Monoclonal Fibromodulin antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23029-174
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465), expandable thumbnail

    Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12623288, 8895216)

    Negative control: Hut-78 (PMID: 15741214)

    Exposure time: Lane 1 second; Lanes 2-6:3 seconds

    All lanes: Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465) at 1/1000 dilution

    Lane 1: Human articular cartilage lysate at 20 µg

    Lane 2: Human uterus lysate at 20 µg

    Lane 3: Human skin lysate at 20 µg

    Lane 4: HuT-78 (human sezary syndrome cutaneous t lymphocyte) whole cell lysate at 20 µg

    Lane 5: Mouse articular cartilage lysate at 20 µg

    Lane 6: Rat articular cartilage lysate at 20 µg

    Secondary

    Lanes 1 - 4: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Lanes 5 - 6: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Predicted band size: 43 kDa

    Observed band size: 60-100 kDa

  • Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465), expandable thumbnail

    Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12623288, 8895216).

    Two bands of 46kDa and 40kDa are de-glycosylated forms. The bands of 60-100kDa are glycosylated forms.

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465) at 1/1000 dilution

    Lane 1: Human uterus tissue lysate at 20 µg

    Lane 2: Human uterus tissue treated with PNGase F lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 43 kDa

    Observed band size: 60-100 kDa

  • Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465), expandable thumbnail

    Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST Exposure time: 114 seconds

    All lanes: Western blot - Anti-Fibromodulin antibody [EPR23029-174] (ab267465) at 1/1000 dilution

    All lanes: Recombinant Human Fibromodulin/FMOD protein (aa75-376) 20ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa

    Observed band size: 35 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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