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Mouse Recombinant Monoclonal Fibronectin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-Fibronectin antibody [10/Fibronectin] (AB281575), expandable thumbnail
  • Western blot - Anti-Fibronectin antibody [10/Fibronectin] (AB281575), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [10/Fibronectin] (AB281575), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [10/Fibronectin] (AB281575), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [10/Fibronectin] (AB281575), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBIHC-FrICC/IF
Human
Tested
Not recommended
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617).AnastellinBinds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.

Alternative names

Recommended products

Mouse Recombinant Monoclonal Fibronectin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Clone number

10/Fibronectin

Purification technique

Affinity purification Protein A

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Fibronectin antibody [10/Fibronectin] (ab281575), expandable thumbnail

    Western blot - Anti-Fibronectin antibody [10/Fibronectin] (ab281575)

    Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS

    The expression molecular weight observed is consistent with what has been described in the literature (PMID:28367057).

    Lanes 1-3: Merged signal (red and green). Green - ab281575 observed at 285kDa. Red - loading control Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker ab52901(Rabbit monoclonal [EP1331Y] to beta Tubulin) observed at 50 kDa.

    Lanes 1-2: ab281575 Anti-Fibronectin antibody was shown to react with Fibronectin in HAP1 cells in Western blot. Loss of signal was observed when Fibronectin knockout sample was used. Wild-type and Fibronectin knockout samples were subjected to SDS-PAGE.

    ab281575 and Anti-beta Tubulin antibody [EP1331Y] - Loading Control (Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker ab52901) were incubated at 4°C overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Fibronectin antibody [10/Fibronectin] (ab281575) at 1/500 dilution

    Lane 1: Wild type HAP1(human chronic myelogenous leukemia near-haploid cell line), whole cell lysate at 20 µg

    Lane 2: Fibronectin knockout HAP1(human chronic myelogenous leukemia near-haploid cell line), whole cell lysate at 20 µg

    Lane 3: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Mouse IgG H&L (IRDye® 800CW) (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution

    Predicted band size: 262 kDa

    Observed band size: 285 kDa

  • Western blot - Anti-Fibronectin antibody [10/Fibronectin] (ab281575), expandable thumbnail

    Western blot - Anti-Fibronectin antibody [10/Fibronectin] (ab281575)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Samples are non-boiled as boiling may cause protein aggregates.

    The expression molecular weight observed is consistent with what has been described in the literature (PMID:28367057).

    Exposure time: lane 1: 3 secondslane 2: 37 seconds

    All lanes: Western blot - Anti-Fibronectin antibody [10/Fibronectin] (ab281575) at 1/1000 dilution

    Lane 1: Human plasma lysate at 20 µg

    Lane 2: U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate at 40 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 262 kDa

    Observed band size: 285 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [10/Fibronectin] (ab281575), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [10/Fibronectin] (ab281575)

    Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Fibronectin with abab281575 at 1/2000 (0.551 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in stromal cells of human colon cancer.The section was incubated with ab281575 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BONDRX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [10/Fibronectin] (ab281575), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [10/Fibronectin] (ab281575)

    Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling Fibronectin with ab281575 at 1/2000 (0.551 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) . Positive staining in stromal cells of human ovarian cancer.The section was incubated with ab281575 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [10/Fibronectin] (ab281575), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [10/Fibronectin] (ab281575)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Fibronectin with ab281575 at 1/2000 (0.551 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) . Positive staining in stromal cells of human testis.The section was incubated with ab281575 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) .

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Western blot - Anti-Fibronectin antibody [10/Fibronectin] (ab281575), expandable thumbnail

    Western blot - Anti-Fibronectin antibody [10/Fibronectin] (ab281575)

    Western blot: Mouse Monoclonal[10/Fibronectin] to Fibronectin ab281575 staining at 1/500 dilution, shown in green; Rabbit anti-ACTN4 (Anti-alpha Actinin 4 antibody [EPR2533(2)] ab108198) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 238-268 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in FN1 knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20,000 dilution.

    All lanes: Western blot - Anti-Fibronectin antibody [10/Fibronectin] (ab281575) at 1/500 dilution

    Lane 1: Wild-type MCF7 at 20 µg

    Lane 2: Western blot - Human FN1 knockout MCF7 cell line (Human FN1 knockout MCF7 cell line ab286324) at 20 µg

    Lane 3: HepG2 at 20 µg

    Lane 4: PC-3 at 20 µg

    Secondary

    All lanes: Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 272 kDa

    Observed band size: 238 kDa, 268 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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