Anti-Fibronectin antibody ab2413 is a rabbit polyclonal antibody that is used in Fibronectin western blotting, IHC and immunofluorescence. Suitable for human and mouse samples.
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- International journal of molecular sciences 24:2023In Vitro and In Vivo Evidence towards Fibronectin's Protective Effects against Prion Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM Carmen Garza,Sang-Gyun Kang,Chiye Kim,Eva Monleón,Jacques van der Merwe,David A Kramer,Richard Fahlman,Valerie L Sim,Judd Aiken,Debbie McKenzie,Leonardo M Cortez,Holger WillePubMed 38139358
- Investigative ophthalmology & visual science 64:122023Lanosterol Synthase Prevents EMT During Lens Epithelial Fibrosis Via Regulating SREBP1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengjuan Ma,Jingqi Huang,Baoxin Chen,Mi Huang,Lang Xiong,Jieping Chen,Shan Huang,Yizhi LiuPubMed 38079167
- Nature communications 14:80692023Systemically administered wound-homing peptide accelerates wound healing by modulating syndecan-4 function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHoracio Maldonado,Bryan D Savage,Harlan R Barker,Ulrike May,Maria Vähätupa,Rahul K Badiani,Katarzyna I Wolanska,Craig M J Turner,Toini Pemmari,Tuomo Ketomäki,Stuart Prince,Martin J Humphries,Erkki Ruoslahti,Mark R Morgan,Tero A H JärvinenPubMed 38057316
- Journal of experimental & clinical cancer research : CR 42:3282023Unveiling the role of osteosarcoma-derived secretome in premetastatic lung remodelling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara F F Almeida,Liliana Santos,Gabriela Sampaio-Ribeiro,Hugo R S Ferreira,Nuno Lima,Rui Caetano,Mónica Abreu,Mónica Zuzarte,Ana Sofia Ribeiro,Artur Paiva,Tânia Martins-Marques,Paulo Teixeira,Rui Almeida,José Manuel Casanova,Henrique Girão,Antero J Abrunhosa,Célia M GomesPubMed 38031171
- Nutrients 15:2023Impact of Ring Finger Protein 20 and Its Downstream Regulation on Renal Tubular Injury in a Unilateral Nephrectomy Mouse Model Fed a High-Fat Diet.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYou-Jin Kim,Se-Hyun Oh,Jeong-Hoon Lim,Jang-Hee Cho,Hee-Yeon Jung,Chan-Duck Kim,Sun-Hee Park,Tae-Hwan Kwon,Yong-Lim KimPubMed 38068817
- Nature communications 14:78142023MicroRNA-mediated attenuation of branched-chain amino acid catabolism promotes ferroptosis in chronic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHisakatsu Sone,Tae Jin Lee,Byung Rho Lee,Dan Heo,Sekyung Oh,Sang-Ho KwonPubMed 38016961
- International journal of nanomedicine 18:7047-70632023Exosome Derived from Mesenchymal Stem Cells Alleviates Hypertrophic Scar by Inhibiting the Fibroblasts via TNFSF-13/HSPG2 Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuimin Zhang,Chengyu Zang,Wen Zhao,Linfeng Zhang,Rui Liu,Zhang Feng,Jie Wu,Rongtao CuiPubMed 38046235
- Frontiers in immunology 14:12594342023Myeloid PFKFB3-mediated glycolysis promotes kidney fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiuhua Yang,Emily Huo,Yongfeng Cai,Zhidan Zhang,Charles Dong,John M Asara,Huidong Shi,Qingqing WeiPubMed 38035106
- Bioactive materials 32:488-5012023Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Zhang,Zhuo Wan,Di Qu,Wenqi Sun,Liang Zhang,Yuan Liang,Lei Pan,Hua Jiang,Zichen Ye,Mengying Wei,Lijun Yuan,Guodong Yang,Faguang JinPubMed 37965241
- Nature communications 14:73492023TLR7 promotes smoke-induced experimental lung damage through the activity of mast cell tryptase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGang Liu,Tatt Jhong Haw,Malcolm R Starkey,Ashleigh M Philp,Stelios Pavlidis,Christina Nalkurthi,Prema M Nair,Henry M Gomez,Irwan Hanish,Alan Cy Hsu,Elinor Hortle,Sophie Pickles,Joselyn Rojas-Quintero,Raul San Jose Estepar,Jacqueline E Marshall,Richard Y Kim,Adam M Collison,Joerg Mattes,Sobia Idrees,Alen Faiz,Nicole G Hansbro,Ryutaro Fukui,Yusuke Murakami,Hong Sheng Cheng,Nguan Soon Tan,Sanjay H Chotirmall,Jay C Horvat,Paul S Foster,Brian Gg Oliver,Francesca Polverino,Antonio Ieni,Francesco Monaco,Gaetano Caramori,Sukhwinder S Sohal,Ken R Bracke,Peter A Wark,Ian M Adcock,Kensuke Miyake,Don D Sin,Philip M HansbroPubMed 37963864
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.5
Preservative: 0.05% Sodium azide
Constituents: 99.854% PBS, 0.096% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Native Full Length Protein corresponding to Human FN1. Database link P02751
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
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Target data
Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617).AnastellinBinds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.
Alternative names
FN, FN, FN1, Fibronectin, FN, Cold-insoluble globulin, CIG
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Anti-Fibronectin antibody ab2413 is a rabbit polyclonal antibody that is used in Fibronectin western blotting, IHC and immunofluorescence. Suitable for human and mouse samples.
- Tried and trusted by researchers since 2002
Same trusted quality, new lower price
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.5
Preservative: 0.05% Sodium azide
Constituents: 99.854% PBS, 0.096% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Native Full Length Protein corresponding to Human FN1. Database link P02751
- Purification technique
Affinity purification
- Light chain type
unknown
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody (ab2413)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Fibronectin with ab2413 at 1/50 dilution. Antigen retrieval performed with EDTA buffer pH 8 before commencing with IHC staining protocol.
Western blot - Anti-Fibronectin antibody (ab2413)
All lanes: Western blot - Anti-Fibronectin antibody (ab2413) at 1 µg/mL
Lane 1: Human colon tissue lysate at 10 µg
Lane 2: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 262 kDa
Observed band size: 285 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody (ab2413)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue, staining Fibronectin with ab2413.
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody (ab2413)
ICC/IF image of ab2413 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2413, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody (ab2413)
ab2413 staining Fibronectin in human kidney tissue section by IHC-P (Formalin/PFA-fixed paraffin embedded tissue sections). Tissue sections were incubated with ab2413 at a dilution of 1:250 for one hour. Heat mediated antigen retrieval technique was used with citrate buffer at pH 6.0. DAB staining was done with a biotinylated secondary for 45 min at RT at a concentration of 1:1000.
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