AB299

Anti-Fibronectin antibody

Name: Anti-Fibronectin antibody (ab299) | Abcam
Brand: Abcam Limited
SKU: AB299
Price: 400 USD
Availability: InStock
Rating: 5 (2 reviews)

Lab Essentials
What is this?

(2 Reviews)

(22 Publications)

Rabbit Polyclonal Fibronectin antibody. Suitable for sELISA, ICC/IF and reacts with Human samples. Cited in 22 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human FN1.

View Alternative Names

FN, FN1, Fibronectin, Cold-insoluble globulin, CIG

1 Images

Sandwich ELISA - Anti-Fibronectin antibody (AB299)

sELISA

Unknown

Sandwich ELISA - Anti-Fibronectin antibody (AB299)

Standard Curve for Fibronectin (Analyte : Fibronectin protein (Human) (ab81743)); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [HFN 7.1] to Fibronectin - BSA and Azide free (ab80923) at 1μg/ml and Detector Antibody Rabbit polyclonal to Fibronectin (ab299) at 0.5μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

sELISA, ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Human FN1.

P02751

Specificity

Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.5 µg/mL", "sELISA-species-notes": "<p>For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [HFN 7.1] to Fibronectin - BSA and Azide free (<a href='/en-us/products/unavailable/fibronectin-antibody-hfn-71-ab80923'>ab80923</a>) as Capture.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. This product has been sterile filtered.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Add glycerol to a final volume of 50% for extra stability and aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed : 34089617).. Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5 : ITGB1 integrin receptor signaling.

See full target information FN1

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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & medicine 22: PubMed39907104

2025

Carnitine palmitoyltransferase 1C promotes EMT-associated cisplatin resistance in non-small cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Renjie Chen,Jiahui Wang,Shuoyu Huang,Xuefeng Hu,Xinran He,Tiange Zhang,Yunhan Hu,Huijun Wei,Sihui Nian,Yushu Huang,Zhihao Wu

BMC veterinary research 18:126 PubMed35366872

2022

NADH oxidase of Mycoplasma hyopneumoniae functions as a potential mediator of virulence.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Hao,Xing Xie,Zhixin Feng,Rong Chen,Yanna Wei,Jin Liu,Qiyan Xiong,Guoqing Shao,Johnson Lin

Frontiers in microbiology 12:747421 PubMed34671334

2021

Nicotinamide Adenine Dinucleotide-Dependent Flavin Oxidoreductase of Functions as a Potential Novel Virulence Factor and Not Only as a Metabolic Enzyme.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Xie,Fei Hao,Rong Chen,Jingjing Wang,Yanna Wei,Jin Liu,Haiyan Wang,Zhenzhen Zhang,Yun Bai,Guoqing Shao,Qiyan Xiong,Zhixin Feng

Journal of cancer research and clinical oncology : PubMed33471184

2021

Resveratrol induces PD-L1 expression through snail-driven activation of Wnt pathway in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Yang,Zongyu Li,Jianping Tao,Hao Hu,Zilin Li,Zhijian Zhang,Feng Cheng,Yihang Sun,Yao Zhang,Jianke Yang,Huijun Wei,Zhihao Wu

Methods in molecular biology (Clifton, N.J.) 2054:23-57 PubMed31482446

2019

Bioengineered Microtissue Models of the Human Bone Metastatic Microenvironment: A Novel In Vitro Theranostics Platform for Cancer Research.

Applications

Unspecified application

Species

Unspecified reactive species

Nathalie Bock

Journal of experimental & clinical cancer research 37:39 PubMed29482580

2018

Upregulation of lactate-inducible snail protein suppresses oncogene-mediated senescence through p16 inactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangrui Li,Zhijian Zhang,Yao Zhang,Yuxiang Cao,Huijun Wei,Zhihao Wu

Nature materials 16:953-961 PubMed28783156

2017

Hydrogels with precisely controlled integrin activation dictate vascular patterning and permeability.

Applications

Unspecified application

Species

Unspecified reactive species

Shuoran Li,Lina R Nih,Haylee Bachman,Peng Fei,Yilei Li,Eunwoo Nam,Robert Dimatteo,S Thomas Carmichael,Thomas H Barker,Tatiana Segura

PloS one 12:e0181151 PubMed28719644

2017

Isolation of putative stem cells present in human adult olfactory mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Tamara Tanos,Alberto Maria Saibene,Carlotta Pipolo,Paolo Battaglia,Giovanni Felisati,Alicia Rubio

Cell death & disease 7:e2302 PubMed27441654

2016

Tankyrase inhibition aggravates kidney injury in the absence of CD2AP.

Applications

Unspecified application

Species

Unspecified reactive species

S Kuusela,H Wang,A A Wasik,H Suleiman,S Lehtonen

PloS one 11:e0147867 PubMed26828433

2016

The Salivary Scavenger and Agglutinin (SALSA) in Healthy and Complicated Pregnancy.

Applications

IHC

Species

Unspecified reactive species

Martin Parnov Reichhardt,Hanna Jarva,Anna Inkeri Lokki,Hannele Laivuori,Piia Vuorela,Vuokko Loimaranta,Andreas Glasner,Monika Siwetz,Berthold Huppertz,Seppo Meri

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fibronectin antibody

Sandwich ELISA - Anti-Fibronectin antibody (AB299)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (22)

Product promise