Rabbit Monoclonal Fibronectin antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat samples. Cited in 13 publications.
Images
![Western blot - Anti-Fibronectin antibody [EPR19241-46] (AB199056), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-ab199056--western-blot-img35204.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fibronectin antibody [EPR19241-46] (AB199056)")
![Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [EPR19241-46] (AB199056), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-ab199056--immunocytochemistry-immunofluorescence-img108419.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [EPR19241-46] (AB199056)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] (AB199056), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-ab199056--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119533.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] (AB199056)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] (AB199056), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-ab199056--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111815.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] (AB199056)")
![Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] (AB199056), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-ab199056--immunoprecipitation-img11628.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] (AB199056)")
Publications
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- Cell reports 42:1124732023Initiation of fibronectin fibrillogenesis is an enzyme-dependent process.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShay Melamed,Shelly Zaffryar-Eilot,Elisabeth Nadjar-Boger,Rohtem Aviram,Huaning Zhao,Wesal Yaseen-Badarne,Rotem Kalev-Altman,Dalit Sela-Donenfeld,Oded Lewinson,Sophie Astrof,Peleg Hasson,Haguy WolfensonPubMed 37148241
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e227942023miR-124-3p improves mitochondrial function of renal tubular epithelial cells in db/db mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuqun Liang,Chunxin Wo,Yao Yuan,Hongjuan Cao,Wanlin Tan,Xingcheng Zhou,Dan Wang,Rongyu Chen,Mingjun Shi,Fan Zhang,Ying Xiao,Lingling Liu,Yuxia Zhou,Tian Zhang,Yuanyuan Wang,Bing GuoPubMed 36753399
- Journal of cell science 135:2022A new mechanism of fibronectin fibril assembly revealed by live imaging and super-resolution microscopy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDarshika Tomer,Cecilia Arriagada,Sudipto Munshi,Brianna E Alexander,Brenda French,Pavan Vedula,Valentina Caorsi,Andrew House,Murat Guvendiren,Anna Kashina,Jean E Schwarzbauer,Sophie AstrofPubMed 35851804
- Stem cells international 2022:19407612022Extracellular Vesicles Derived from Adipose Mesenchymal Stem Cells Promote Peritoneal Healing by Activating MAPK-ERK1/2 and PI3K-Akt to Alleviate Postoperative Abdominal Adhesion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManyu Shi,Hengchen Liu,Tingting Zhang,Mingzhao Zhang,Xin Tang,Zenan Zhang,Wenjun Lu,Shulong Yang,Zhitao Jiang,Qingbo Cui,Zhaozhu LiPubMed 35578661
- Nature communications 12:61322021StemBond hydrogels control the mechanical microenvironment for pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCéline Labouesse,Bao Xiu Tan,Chibeza C Agley,Moritz Hofer,Alexander K Winkel,Giuliano G Stirparo,Hannah T Stuart,Christophe M Verstreken,Carla Mulas,William Mansfield,Paul Bertone,Kristian Franze,José C R Silva,Kevin J ChalutPubMed 34675200
- Cells 10:2021Functional Recellularization of Acellular Rat Liver Scaffold by Induced Pluripotent Stem Cells: Molecular Evidence for Wnt/B-Catenin Upregulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNesrine Ebrahim,Omnia A M Badr,Mohamed M Yousef,Amira Hassouna,Dina Sabry,Ayman Samir Farid,Ola Mostafa,Hajir A Al Saihati,Yasmin Seleem,Eman Abd El Aziz,Ahmed Hassan Khalil,Ahmed Nawar,Ahmed A Shoulah,Mohammad Aljasir,Amira Zaki Mohamed,Mohamed El-Sherbiny,Nehal M Elsherbiny,Mohamed Ahmed Eladl,Nicholas Robert Forsyth,Rabab F SalimPubMed 34831042
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e21002332021Hyperbaric Oxygen Boosts PD-1 Antibody Delivery and T Cell Infiltration for Augmented Immune Responses Against Solid Tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Liu,Ningbing Ye,Sha Liu,Jiankun Guan,Qingyuan Deng,Zhijie Zhang,Chen Xiao,Ze-Yang Ding,Bi-Xiang Zhang,Xiao-Ping Chen,Zifu Li,Xiangliang YangPubMed 34085419
- Gastroenterology 159:1019-1035.e222020Protein Kinase D1, Reduced in Human Pancreatic Tumors, Increases Secretion of Small Extracellular Vesicles From Cancer Cells That Promote Metastasis to Lung in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMilena Armacki et. al.PubMed 32446697
- Marine drugs 17:2019Fucoxanthin Alleviates Oxidative Stress through Akt/Sirt1/FoxO3α Signaling to Inhibit HG-Induced Renal Fibrosis in GMCs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuanyu Yang,Lin Jin,Dongxiao Zheng,Xiaoliang Tang,Junwei Yang,Lingxuan Fan,Xi XiePubMed 31842414
- Kidney research and clinical practice 38:472-4802019Effects of tranilast on the epithelial-to-mesenchymal transition in peritoneal mesothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeok Hui Kang,Sang Woon Kim,Keuk Jun Kim,Kyu Hyang Cho,Jong Won Park,Chan-Duck Kim,Jun Young DoPubMed 31554027
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Mouse | Tested | Tested | Tested | Tested | Tested |
| Rat | Expected | Tested | Expected | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/250 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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15 products for Alternative Product
Target data
Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (By similarity). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape (By similarity). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (PubMed:21768292). Participates in the regulation of type I collagen deposition by osteoblasts (PubMed:21768292). Acts as a ligand for the Lilrb4a receptor, inhibiting Fcgr1/CD64-mediated monocyte activation (PubMed:34089617).Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.AnastellinBinds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
Alternative names
Fibronectin, FN, Fn1
Recommended products
Rabbit Monoclonal Fibronectin antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat samples. Cited in 13 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR19241-46
- Purification technique
Affinity purification Protein A
- Specificity
Western blot and IHC tests of human samples were negative.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Fibronectin antibody [EPR19241-46] (ab199056), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-ab199056--western-blot-img35204.jpg "Western blot - Anti-Fibronectin antibody [EPR19241-46] (ab199056)")
Western blot - Anti-Fibronectin antibody [EPR19241-46] (ab199056)
Exposure time : Lanes 1 and 2:4 seconds; Lanes 3,4 and 5:2 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 6994838, PMID 7516997, PMID: 21923916).
All lanes: Western blot - Anti-Fibronectin antibody [EPR19241-46] (ab199056) at 1/1000 dilution
Lane 1: Mouse liver lysate at 10 µL/mL Buffer
Lane 2: Rat liver lysate at 10 µL/mL Buffer
Lane 3: Mouse plasma at 10 µL/mL Buffer
Lane 4: Rat plasma at 10 µL/mL Buffer
Lane 5: Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate at 10 µL/mL Buffer
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 262 kDa
Observed band size: 220-273 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [EPR19241-46] (ab199056), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-ab199056--immunocytochemistry-immunofluorescence-img108419.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [EPR19241-46] (ab199056)")
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [EPR19241-46] (ab199056)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hepa 1-6 (mouse hepatoma epithelial cell line) cells labeling Fibronectin with ab199056 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining on Hepa1-6 cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] (ab199056), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-ab199056--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119533.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] (ab199056)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] (ab199056)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Fibronectin with ab199056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the extracellular matrix of mouse stomach (PMID: 650151).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] (ab199056), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-ab199056--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111815.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] (ab199056)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] (ab199056)
Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Fironectin with ab199056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the extracellular matrix of rat stomach (PMID: 650151).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] (ab199056), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-ab199056--immunoprecipitation-img11628.jpg "Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] (ab199056)")
Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] (ab199056)
Fibronectin was immunoprecipitated from 0.35 mg of mouse plasma with ab199056 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199056 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse plasma 10 μg (Input).
Lane 2: ab199056 IP in mouse plasma.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab199056 in mouse plasma.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
All lanes: Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] (ab199056)
Predicted band size: 262 kDa
![Flow Cytometry (Intracellular) - Anti-Fibronectin antibody [EPR19241-46] (ab199056), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-ab199056--flow-cytometry-intracellular-img414511.jpg "Flow Cytometry (Intracellular) - Anti-Fibronectin antibody [EPR19241-46] (ab199056)")
Flow Cytometry (Intracellular) - Anti-Fibronectin antibody [EPR19241-46] (ab199056)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling Fibronectin with ab199056 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluorr® 488) at 1/2000 dilution was used as the secondary antibody.
![Flow Cytometry (Intracellular) - Anti-Fibronectin antibody [EPR19241-46] (ab199056), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-ab199056--flow-cytometry-intracellular-img414511.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Fibronectin antibody [EPR19241-46] (ab199056)")
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