Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (AB224689)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Firbonectin with ab199056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the extracellular matrix of mouse stomach (PMID : 650151).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199056).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (AB224689)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Fironectin with ab199056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the extracellular matrix of rat stomach (PMID : 650151).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199056).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (AB224689)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hepa 1-6 (mouse hepatoma epithelial cell line) cells labeling Fibronectin with ab199056 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining on Hepa1-6 cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199056).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (AB224689)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling Fibronectin with ab199056 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199056).

• IP

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Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (AB224689)

Fibronectin was immunoprecipitated from 0.35 mg of mouse plasma with ab199056 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199056 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse plasma 10 μg (Input).

Lane 2 : ab199056 IP in mouse plasma.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199056 in mouse plasma.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199056).

All lanes:

Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] (<a href='/en-us/products/primary-antibodies/fibronectin-antibody-epr19241-46-ab199056'>ab199056</a>)

Predicted band size: 262 kDa

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