Rabbit Monoclonal Fibronectin antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (AB224689), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-bsa-and-azide-free-ab224689--immunocytochemistry-immunofluorescence-img108419.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (AB224689)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (AB224689), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-bsa-and-azide-free-ab224689--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119533.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (AB224689)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (AB224689), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-bsa-and-azide-free-ab224689--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111815.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (AB224689)")
![Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (AB224689), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-bsa-and-azide-free-ab224689--immunoprecipitation-img11628.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (AB224689)")
![Flow Cytometry (Intracellular) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (AB224689), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-bsa-and-azide-free-ab224689--flow-cytometry-intracellular-img414511.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (AB224689)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Mouse | Tested | Expected | Tested | Tested | Tested |
| Rat | Predicted | Expected | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
10 products for Alternative Product
3 products for Alternative Version
Target data
Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (By similarity). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape (By similarity). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (PubMed:21768292). Participates in the regulation of type I collagen deposition by osteoblasts (PubMed:21768292). Acts as a ligand for the Lilrb4a receptor, inhibiting Fcgr1/CD64-mediated monocyte activation (PubMed:34089617). Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling. Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
Alternative names
Fibronectin, FN, Fn1
Recommended products
Rabbit Monoclonal Fibronectin antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19241-46
- Purification technique
- Affinity purification Protein A
- Specificity
Western blot and IHC tests of human samples were negative.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab224689 is the carrier-free version of Anti-Fibronectin antibody [EPR19241-46] ab199056.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-bsa-and-azide-free-ab224689--immunocytochemistry-immunofluorescence-img108419.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)")
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hepa 1-6 (mouse hepatoma epithelial cell line) cells labeling Fibronectin with Anti-Fibronectin antibody [EPR19241-46] ab199056 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining on Hepa1-6 cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibronectin antibody [EPR19241-46] ab199056).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-bsa-and-azide-free-ab224689--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119533.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Firbonectin with Anti-Fibronectin antibody [EPR19241-46] ab199056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the extracellular matrix of mouse stomach (PMID: 650151).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibronectin antibody [EPR19241-46] ab199056).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-bsa-and-azide-free-ab224689--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111815.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)
Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Fironectin with Anti-Fibronectin antibody [EPR19241-46] ab199056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the extracellular matrix of rat stomach (PMID: 650151).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibronectin antibody [EPR19241-46] ab199056).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-bsa-and-azide-free-ab224689--immunoprecipitation-img11628.jpg "Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)")
Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)
Fibronectin was immunoprecipitated from 0.35 mg of mouse plasma with Anti-Fibronectin antibody [EPR19241-46] ab199056 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Fibronectin antibody [EPR19241-46] ab199056 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse plasma 10 μg (Input).
Lane 2: Anti-Fibronectin antibody [EPR19241-46] ab199056 IP in mouse plasma.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Fibronectin antibody [EPR19241-46] ab199056 in mouse plasma.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibronectin antibody [EPR19241-46] ab199056).
All lanes: Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] (Anti-Fibronectin antibody [EPR19241-46] ab199056)
Predicted band size: 262 kDa
![Flow Cytometry (Intracellular) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-epr19241-46-bsa-and-azide-free-ab224689--flow-cytometry-intracellular-img414511.jpg "Flow Cytometry (Intracellular) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)")
Flow Cytometry (Intracellular) - Anti-Fibronectin antibody [EPR19241-46] - BSA and Azide free (ab224689)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling Fibronectin with Anti-Fibronectin antibody [EPR19241-46] ab199056 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Fibronectin antibody [EPR19241-46] ab199056).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com