Name: Anti-Fibronectin antibody [EPR23110-46]
SKU: ab268020
Rating: 4 (5 reviews)

Anti-Fibronectin antibody [EPR23110-46] (ab268020) is a rabbit monoclonal antibody detecting Fibronectin in Western Blot, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 40 publications

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Anti-Fibronectin antibody [EPR23110-46] - BSA and Azide free
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Alexa Fluor® 594 Anti-Fibronectin antibody [EPR23110-46]
ab313350
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Alexa Fluor® 647 Anti-Fibronectin antibody [EPR23110-46]
ab313020
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Alexa Fluor® 568 Anti-Fibronectin antibody [EPR23110-46]
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Alexa Fluor® 555 Anti-Fibronectin antibody [EPR23110-46]
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Conjugated
Alexa Fluor® 488 Anti-Fibronectin antibody [EPR23110-46]
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Conjugated
Alexa Fluor® 750 Anti-Fibronectin antibody [EPR23110-46]

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (AB268020), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt	WB	ICC/IF	IHC-Fr	IHC-P
Human	Not recommended	Not recommended	Tested	Tested	Expected	Tested
Mouse	Not recommended	Not recommended	Tested	Tested	Tested	Tested
Rat	Not recommended	Not recommended	Tested	Expected	Tested	Tested

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes This product is not suitable for testing mouse and rat tissue samples in western blot.
Species Rat	Dilution info 1/1000	Notes This product is not suitable for testing mouse and rat tissue samples in western blot.
Species Human	Dilution info 1/1000	Notes This product is not suitable for testing mouse and rat tissue samples in western blot.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -
Species Human	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Species Rat	Dilution info 1/500	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

See full target information FN1

Function

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617). Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.

Alternative names

FN, FN1, Fibronectin, Cold-insoluble globulin, CIG

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR23110-46
Purification technique: Affinity purification Protein A
Specificity: This product is not suitable for testing mouse and rat tissue samples in western blot.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Anti-Fibronectin antibody [EPR23110-46] (ab268020) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-Fr, IHC-P, WB in human, mouse, rat samples.

Anti-Fibronectin antibody [EPR23110-46] (ab268020) has been cited over 46 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality manufacturing and validation processes ensure Anti-Fibronectin antibody [EPR23110-46] (ab268020) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

The specificity of Anti-Fibronectin antibody [EPR23110-46] (ab268020) has been confirmed by testing in knockout samples.

Anti-Fibronectin antibody [EPR23110-46] (ab268020) specifically detects Fibronectin (UniProt ID: P02751; Molecular weight: 269kDa) and is sold in 100 uL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR23110-46 - ab26822.

Antibody clone EPR23110-46 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 594, Alexa Fluor® 568, Alexa Fluor® 555, Alexa Fluor® 647, Alexa Fluor® 488, Alexa Fluor® 75 (ab3964, ab3132, Alexa Fluor® 555 Anti-Fibronectin antibody [EPR23110-46] ab313223, ab31335, Alexa Fluor® 488 Anti-Fibronectin antibody [EPR23110-46] ab314679, Alexa Fluor® 750 Anti-Fibronectin antibody [EPR23110-46] ab321527).

Fibronectin is a crucial extracellular matrix protein that significantly influences tumor development and progression by enhancing tumor growth, metastasis, and angiogenesis. It interacts with integrin receptors to help tumor cells adapt and survive in harsh microenvironments.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (ab268020)
Immunohistochemical analysis of paraffin-embedded human esophageal squamous cell carcinoma tissue labeling Fibronectin with ab268020 at 1/2000 dilution (0.29 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in the stroma of human esophageal squamous cell carcinoma (PMID:30314454). Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (ab268020)
Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Fibronectin with ab268020 at 1/2000 dilution (0.29 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in the smooth muscle of rat stomach (PMID:650101) Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (ab268020)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Fibronectin with ab268020 at 1/2000 dilution (0.29 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in the smooth muscle of mouse stomach (PMID:650101) Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (ab268020)
Immunohistochemical analysis of paraffin-embedded human mammary gland tissue labeling Fibronectin with ab268020 at 1/2000 dilution (0.29 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in the stroma of human mammary gland (PMID:650101). Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [EPR23110-46] (ab268020)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling Fibronectin with ab268020 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cells is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-Fibronectin antibody [EPR23110-46] (ab268020)
Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat colon tissue labeling Fibronectin with ab268020 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining mainly on smooth muscle of rat colon (PMID: 17881565) is observed. The nuclear counterstain is DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Western blot - Anti-Fibronectin antibody [EPR23110-46] (ab268020)
ab268020 was shown to specifically react with Fibronectin in wild-type HAP1 cells as signal was lost in Fibronectin knockout cells. Wild-type and Fibronectin knockout samples were subjected to SDS-PAGE. ab268020 and Anti-Vinculin antibody [EPR8185] - Loading Control ab129002 (Rabbit anti-Vinculin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/5000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Exposure time: Lanes 1-2: 37 seconds; Lanes 3-4: 3 minutes.
Blocking/diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-Fibronectin antibody [EPR23110-46] (ab268020) at 0.585 µg/mL
Lane 1: Human stomach tissue lysate at 10 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg
Lane 3: Fibronectin-1 knockout HAP1 whole cell lysate at 40 µg
Lane 4: Wild-type HAP1 whole cell lysate at 40 µg
Developed using the ECL technique.
Predicted band size: 262 kDa
Observed band size: 285 kDa
Western blot - Anti-Fibronectin antibody [EPR23110-46] (ab268020)
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-2, 5-6: 3 seconds; Lane 3: 125 seconds; Lane 4: 33 seconds.
The expression molecular weight observed is consistent with what has been described in the literature (PMID:28367057).
All lanes: Western blot - Anti-Fibronectin antibody [EPR23110-46] (ab268020) at 1/1000 dilution
Lane 1: Human serum at 20 µg
Lane 2: Human plasma at 20 µg
Lane 3: Human kidney tissue lysate at 20 µg
Lane 4: Human stomach tissue lysate at 20 µg
Lane 5: Mouse plasma at 20 µg
Lane 6: Rat plasma at 20 µg
Secondary
Lanes 1 - 4: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Lanes 5 - 6: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 262 kDa
Observed band size: 285 kDa
Immunohistochemistry (Frozen sections) - Anti-Fibronectin antibody [EPR23110-46] (ab268020)
Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse colon tissue labeling Fibronectin with ab268020 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining mainly on smooth muscle of mouse colon (PMID: 17881565) is observed. The nuclear counterstain is DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [EPR23110-46] (ab268020)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeling Fibronectin with ab268020 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cells is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (ab268020)
Immunohistochemical analysis of paraffin-embedded Human pancreatic carcinoma tissue labeling Fibronectin with ab268020 at 1/4000 dilution (0.146 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). The section was incubated with ab268020 at 4°C overnight. Positive staining in the human pancreatic carcinoma counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP)(Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880)

Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (ab268020)
Immunohistochemical analysis of formalin fixed paraffin embedded human esophageal squamus cell carcinoma labelling fibronectin with ab268020 at a concentration of 0.05µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.
ab268020 anti-fibronectin antibody [EPR23110-46] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).
Western blot - Anti-Fibronectin antibody [EPR23110-46] (ab268020)
Fibronectin Western blot staining using rabbit Anti-Fibronectin antibody
Western blot: Rabbit Monoclonal[EPR23110-46] to Fibronectin ab268020 staining at 1/1000 dilution, shown in green; Mouse anti-CANX (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 238-268 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in FN1 knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.
All lanes: Western blot - Anti-Fibronectin antibody [EPR23110-46] (ab268020) at 1/1000 dilution
Lane 1: Wild-type MCF7 at 20 µg
Lane 2: Western blot - Human FN1 knockout MCF7 cell line (ab286324) at 20 µg
Lane 3: HepG2 at 20 µg
Lane 4: PC-3 at 20 µg
Secondary
All lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 272 kDa
Observed band size: 238 kDa, 268 kDa