Mouse Monoclonal Fibronectin antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Cell preparation containing FN1 protein.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [Fn-3] (AB18265), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-fn-3-ab18265--immunocytochemistry-immunofluorescence-img118981.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [Fn-3] (AB18265)")
Publications
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- Current issues in molecular biology 44:5048-50662022Protective Effects of One 2,4-Dihydro-3H-Pyrazol-3-one Derivative against Posterior Capsular Opacification by Regulation of TGF-β2/SMADs and Non-SMAD Signaling, Collagen I, and Fibronectin Proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Ching Shih,Chia-Yi Lee,Fung-Fuh Wong,Cheng-Hsiu LinPubMed 36286058
- Journal for immunotherapy of cancer 10:2022Ultrasound combined with nanobubbles promotes systemic anticancer immunity and augments anti-PD1 efficacy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianjun Hu,Jiangyi He,Yunlong Wang,Yang Zhao,Kejing Fang,Yan Dong,Yanrong Chen,Yue Zhang,Chi Zhang,Hongwei Wang,Jun Tan,Junyi Wang,Ruiyang Zi,Chengxiang Liu,Houjie Liang,Yanli Guo,Juanjuan OuPubMed 35236741
- Drug design, development and therapy 14:395-4052020Genipin Induces Autophagy and Suppresses Cell Growth of Oral Squamous Cell Carcinoma via PI3K/AKT/MTOR Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingBo Wei et. al.PubMed 32099325
- RSC advances 9:37620-376292019Retracted Article: Long non-coding RNA TUG1 alleviates high glucose induced podocyte inflammation, fibrosis and apoptosis in diabetic nephropathy targeting the miR-27a-3p/E2F3 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Li,Denggao Huang,Linlin Zheng,Hui Cao,Yuanhui Gao,Yongmei Yang,Zhongcheng FanPubMed 35542278
- International journal of oncology 54:2157-21682019Long non‑coding RNA H19 regulates cell growth and metastasis via the miR‑22‑3p/Snail1 axis in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Gan et. al.PubMed 31081061
- Molecular medicine reports 19:2817-28242019Long noncoding RNA homeobox A11 antisense promotes transforming growth factor β1‑induced fibrogenesis in cardiac fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiangting Wang et. al.PubMed 30720066
- Scientific reports 7:424612017Bio-functionalized magnetic nanoparticles for the immunoassay of fetal fibronectin: a feasibility study for the prediction of preterm birth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChian-Huey Wong,Chie-Pein Chen,Chia-Chen Chang,Chen-Yu ChenPubMed 28198381
- Oncogene 32:1240-512012Endoplasmic reticulum protein 29 regulates epithelial cell integrity during the mesenchymal-epithelial transition in breast cancer cells.
- Journal of cell science 123:3989-992010Autocrine fibronectin directs matrix assembly and crosstalk between cell-matrix and cell-cell adhesion in vascular endothelial cells.
- Molecular biology & medicine 2:15-271984Monoclonal antibodies that distinguish between human cellular and plasma fibronectin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ Keen,S E Chang,J Taylor-PapadimitriouPubMed 6083431
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Cell preparation containing FN1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P02751
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Associated Products
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Target data
Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617).AnastellinBinds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.
Alternative names
FN, FN, FN1, Fibronectin, FN, Cold-insoluble globulin, CIG
Recommended products
Mouse Monoclonal Fibronectin antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Cell preparation containing FN1 protein.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Cell preparation containing FN1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P02751
- Clone number
Fn-3
- Purification technique
Affinity purification Protein A/G
- Specificity
Reacts with human cellular fibronectin, but not plasma fibronectin.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
![Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [Fn-3] (ab18265), expandable thumbnail](https://content.abcam.com/products/images/fibronectin-antibody-fn-3-ab18265--immunocytochemistry-immunofluorescence-img118981.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [Fn-3] (ab18265)")
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [Fn-3] (ab18265)
ICC/IF image of ab18265 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18265, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
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