AB18265

Anti-Fibronectin antibody [Fn-3]

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(11 Publications)

Mouse Monoclonal Fibronectin antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Cell preparation containing FN1 protein.

View Alternative Names

FN, FN1, Fibronectin, Cold-insoluble globulin, CIG

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [Fn-3] (AB18265)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [Fn-3] (AB18265)

ICC/IF image of ab18265 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18265, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Fn-3

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Cell preparation containing FN1 protein. The exact immunogen used to generate this antibody is proprietary information.

P02751

Specificity

Reacts with human cellular fibronectin, but not plasma fibronectin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed : 34089617).. Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5 : ITGB1 integrin receptor signaling.

See full target information FN1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Current issues in molecular biology 44:5048-5066 PubMed36286058

2022

Protective Effects of One 2,4-Dihydro-3H-Pyrazol-3-one Derivative against Posterior Capsular Opacification by Regulation of TGF-β2/SMADs and Non-SMAD Signaling, Collagen I, and Fibronectin Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Ching Shih,Chia-Yi Lee,Fung-Fuh Wong,Cheng-Hsiu Lin

Journal for immunotherapy of cancer 10: PubMed35236741

2022

Ultrasound combined with nanobubbles promotes systemic anticancer immunity and augments anti-PD1 efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Jianjun Hu,Jiangyi He,Yunlong Wang,Yang Zhao,Kejing Fang,Yan Dong,Yanrong Chen,Yue Zhang,Chi Zhang,Hongwei Wang,Jun Tan,Junyi Wang,Ruiyang Zi,Chengxiang Liu,Houjie Liang,Yanli Guo,Juanjuan Ou

Scientific reports 11:16797 PubMed34408231

2021

Green nanotechnology of MGF-AuNPs for immunomodulatory intervention in prostate cancer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Menka Khoobchandani,Aslam Khan,Kavita K Katti,Velaphi C Thipe,Amal Y Al-Yasiri,Darsha K D MohanDoss,Michael B Nicholl,Ademar B Lugão,Chetan P Hans,Kattesh V Katti

Drug design, development and therapy 14:395-405 PubMed32099325

2020

Genipin Induces Autophagy and Suppresses Cell Growth of Oral Squamous Cell Carcinoma via PI3K/AKT/MTOR Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

MingBo Wei,YanLi Wu,Hui Liu,Chun Xie

RSC advances 9:37620-37629 PubMed35542278

2019

Retracted Article: Long non-coding RNA TUG1 alleviates high glucose induced podocyte inflammation, fibrosis and apoptosis in diabetic nephropathy targeting the miR-27a-3p/E2F3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Denggao Huang,Linlin Zheng,Hui Cao,Yuanhui Gao,Yongmei Yang,Zhongcheng Fan

International journal of oncology 54:2157-2168 PubMed31081061

2019

Long non‑coding RNA H19 regulates cell growth and metastasis via the miR‑22‑3p/Snail1 axis in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Li Gan,Lin Lv,Shengtao Liao

Molecular medicine reports 19:2817-2824 PubMed30720066

2019

Long noncoding RNA homeobox A11 antisense promotes transforming growth factor β1‑induced fibrogenesis in cardiac fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangting Wang,Xiaowei Liu,Qin Zhuang,Rongrong Pan,Linlin Zou,Zhenbo Cen,Lijiang Tang

Scientific reports 7:42461 PubMed28198381

2017

Bio-functionalized magnetic nanoparticles for the immunoassay of fetal fibronectin: a feasibility study for the prediction of preterm birth.

Applications

Unspecified application

Species

Unspecified reactive species

Chian-Huey Wong,Chie-Pein Chen,Chia-Chen Chang,Chen-Yu Chen

Oncogene 32:1240-51 PubMed22543584

2012

Endoplasmic reticulum protein 29 regulates epithelial cell integrity during the mesenchymal-epithelial transition in breast cancer cells.

Applications

Species

Unspecified reactive species

I F Bambang,Y K Lee,D R Richardson,D Zhang

Journal of cell science 123:3989-99 PubMed20980391

2010

Autocrine fibronectin directs matrix assembly and crosstalk between cell-matrix and cell-cell adhesion in vascular endothelial cells.

Applications

ICC/IF

Species

Cow

Botond Cseh,Samantha Fernandez-Sauze,Dominique Grall,Sébastien Schaub,Eszter Doma,Ellen Van Obberghen-Schilling

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fibronectin antibody [Fn-3]

Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [Fn-3] (AB18265)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (11)

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