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Mouse Monoclonal Fibronectin antibody. Suitable for ELISA, WB, ICC/IF and reacts with Recombinant full length protein - Human, Human samples. Immunogen corresponding to Native Full Length Protein corresponding to Human FN1.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [M013] (AB253287), expandable thumbnail
  • Western blot - Anti-Fibronectin antibody [M013] (AB253287), expandable thumbnail
  • ELISA - Anti-Fibronectin antibody [M013] (AB253287), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Native Full Length Protein corresponding to Human FN1. Database link P02751

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAWBICC/IF
Human
Expected
Tested
Tested
Recombinant full length protein - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Target data

Function

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617). Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.

Alternative names

FN, FN1, Fibronectin, Cold-insoluble globulin, CIG

Recommended products

Mouse Monoclonal Fibronectin antibody. Suitable for ELISA, WB, ICC/IF and reacts with Recombinant full length protein - Human, Human samples. Immunogen corresponding to Native Full Length Protein corresponding to Human FN1.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Native Full Length Protein corresponding to Human FN1. Database link P02751
Clone number
M013
Purification technique
Affinity purification Protein G
Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [M013] (ab253287), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [M013] (ab253287)

    Paraformaldehyde-fixed, A549 (Human lung carcinoma cell line) cells labeling Fibronectin using ab253287 at a 1/50 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.

  • Western blot - Anti-Fibronectin antibody [M013] (ab253287), expandable thumbnail

    Western blot - Anti-Fibronectin antibody [M013] (ab253287)

    Native conditions.

    Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hr at room temperature.

    All lanes: Western blot - Anti-Fibronectin antibody [M013] (ab253287) at 1/1000 dilution

    Lane 1: A549 (Human lung carcinoma cell line) cell lysate

    Lane 2: MeWo (Human malignant melanoma cell line) cell lysate

    Lane 3: MDA-MB-231 (Human breast adenocarcinoma cell line) cell lysate

    Lane 4: Human plasma fibronectin

    Predicted band size: 262 kDa

  • ELISA - Anti-Fibronectin antibody [M013] (ab253287), expandable thumbnail

    ELISA - Anti-Fibronectin antibody [M013] (ab253287)

    Representative Standard Curve using ab253287 for ELISA capture of human recombinant fibronectin protein. Capture was detected by using anti-fibronectin ab253287 followed by streptavidin conjugated to HRP.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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