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AB253287

Anti-Fibronectin antibody [M013]

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(1 Publication)

Mouse Monoclonal Fibronectin antibody. Suitable for ELISA, WB, ICC/IF and reacts with Recombinant full length protein - Human, Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human FN1.

View Alternative Names

FN, FN1, Fibronectin, Cold-insoluble globulin, CIG

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [M013] (AB253287)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [M013] (AB253287)

Paraformaldehyde-fixed, A549 (Human lung carcinoma cell line) cells labeling Fibronectin using ab253287 at a 1/50 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.

ELISA - Anti-Fibronectin antibody [M013] (AB253287)
  • ELISA

Supplier Data

ELISA - Anti-Fibronectin antibody [M013] (AB253287)

Representative Standard Curve using ab253287 for ELISA capture of human recombinant fibronectin protein. Capture was detected by using anti-fibronectin ab253287 followed by streptavidin conjugated to HRP.

Western blot - Anti-Fibronectin antibody [M013] (AB253287)
  • WB

Supplier Data

Western blot - Anti-Fibronectin antibody [M013] (AB253287)

Native conditions.

Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hr at room temperature.

All lanes:

Western blot - Anti-Fibronectin antibody [M013] (ab253287) at 1/1000 dilution

Lane 1:

A549 (Human lung carcinoma cell line) cell lysate

Lane 2:

MeWo (Human malignant melanoma cell line) cell lysate

Lane 3:

MDA-MB-231 (Human breast adenocarcinoma cell line) cell lysate

Lane 4:

Human plasma fibronectin

Predicted band size: 262 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

M013

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ELISA, WB, ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Human FN1.

P02751

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Complementary Products

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from TCS.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed : 34089617).. Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5 : ITGB1 integrin receptor signaling.
See full target information FN1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed39940693

2025

Establishment of a Panel of Human Cell Lines to Identify Cellular Receptors Used by to Infect Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anastasiia O Sosnovtseva,Thi Hoa Le,Dmitry S Karpov,Pavel O Vorobyev,Yana D Gumennaya,Olga N Alekseeva,Peter M Chumakov,Anastasia V Lipatova
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Product promise

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For full details, please see our Terms & Conditions

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