AB253288

Anti-Fibronectin antibody [M022]

Lab Essentials
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(3 Publications)

Mouse Monoclonal Fibronectin antibody. Suitable for ELISA, WB, ICC/IF and reacts with Recombinant full length protein - Human, Human samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human FN1.

View Alternative Names

FN, FN1, Fibronectin, Cold-insoluble globulin, CIG

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [M022] (AB253288)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [M022] (AB253288)

Paraformaldehyde-fixed MeWo (Human malignant melanoma cell line) cells labeling Fibronectin using ab253288 at a 1/100 dilution in ICC/IF. Goat anti-mouse DyLight^® 594 secondary.

ELISA - Anti-Fibronectin antibody [M022] (AB253288)

ELISA

Supplier Data

ELISA - Anti-Fibronectin antibody [M022] (AB253288)

Representative Standard Curve using ab253288 for ELISA capture of human recombinant fibronectin protein with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.

Supplier Data

Western blot - Anti-Fibronectin antibody [M022] (AB253288)

Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hr at room temperature.

All lanes:

Western blot - Anti-Fibronectin antibody [M022] (ab253288) at 1/500 dilution

Lane 1:

Human fibronectin purified from plasma

Lane 2:

Human breast tissue lysate

Lane 3:

A549 (Human lung carcinoma cell line) cell lysate

Lane 4:

LNCaP (Human prostate cancer cell line) cell lysate

Predicted band size: 262 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

M022

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, ELISA, WB

applications

Immunogen

Native Full Length Protein corresponding to Human FN1.

P02751

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Recombinant full length protein - Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from TCS.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed : 34089617).. Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5 : ITGB1 integrin receptor signaling.

See full target information FN1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed39940693

2025

Establishment of a Panel of Human Cell Lines to Identify Cellular Receptors Used by to Infect Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anastasiia O Sosnovtseva,Thi Hoa Le,Dmitry S Karpov,Pavel O Vorobyev,Yana D Gumennaya,Olga N Alekseeva,Peter M Chumakov,Anastasia V Lipatova

Journal of neuroinflammation 18:172 PubMed34372877

2021

Increasing toll-like receptor 2 on astrocytes induced by Schwann cell-derived exosomes promotes recovery by inhibiting CSPGs deposition after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Dayu Pan,Yongjin Li,Fuhan Yang,Zenghui Lv,Shibo Zhu,Yixin Shao,Ying Huang,Guangzhi Ning,Shiqing Feng

Journal of clinical laboratory analysis 35:e23857 PubMed34097306

2021

MiR-221-3p and miR-92a-3p enhances smoking-induced inflammation in COPD.

Applications

Unspecified application

Species

Unspecified reactive species

Yahui Shen,Huiyu Lu,Guixian Song

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fibronectin antibody [M022]

Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [M022] (AB253288)

ELISA - Anti-Fibronectin antibody [M022] (AB253288)

Western blot - Anti-Fibronectin antibody [M022] (AB253288)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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