Rabbit Polyclonal Fibronectin antibody. Suitable for WB, ICC/IF, IHC-P, IP and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FN1 aa 350-700.
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Publications
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- Investigative ophthalmology & visual science 64:122023Lanosterol Synthase Prevents EMT During Lens Epithelial Fibrosis Via Regulating SREBP1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengjuan Ma,Jingqi Huang,Baoxin Chen,Mi Huang,Lang Xiong,Jieping Chen,Shan Huang,Yizhi LiuPubMed 38079167
- Cells 11:2022Long Non-Coding RNA Prevents Lens Fibrosis through Maintaining Lens Epithelial Cell Phenotypes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLang Xiong,Yan Sun,Jingqi Huang,Pengjuan Ma,Xiaoran Wang,Jiani Wang,Baoxin Chen,Jieping Chen,Mi Huang,Shan Huang,Yizhi LiuPubMed 36010635
- Investigative ophthalmology & visual science 63:262022TFEB-Mediated Lysosomal Restoration Alleviates High Glucose-Induced Cataracts Via Attenuating Oxidative Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Sun,Xiaoran Wang,Baoxin Chen,Mi Huang,Pengjuan Ma,Lang Xiong,Jingqi Huang,Jieping Chen,Shan Huang,Yizhi LiuPubMed 35758908
- Cell proliferation 53:e129112020MiR-22-3p inhibits fibrotic cataract through inactivation of HDAC6 and increase of α-tubulin acetylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoran Wang et. al.PubMed 32985730
- Molecular medicine reports 20:2929-29352019All‑trans‑retinoic acid modulates TGF‑β‑induced apoptosis, proliferation, migration and extracellular matrix synthesis of conjunctival fibroblasts by inhibiting PI3K/AKT signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingling Liang et. al.PubMed 31322252
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human FN1 aa 350-700. The exact immunogen used to generate this antibody is proprietary information. Database link P02751
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | IHC-P | IP | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected | Expected |
| Rat | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Associated Products
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7 products for Alternative Product
Target data
Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617). Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.
Alternative names
FN, FN1, Fibronectin, Cold-insoluble globulin, CIG
Recommended products
Rabbit Polyclonal Fibronectin antibody. Suitable for WB, ICC/IF, IHC-P, IP and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FN1 aa 350-700.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human FN1 aa 350-700. The exact immunogen used to generate this antibody is proprietary information. Database link P02751
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
Western blot - Anti-Fibronectin antibody - N-terminal (ab137720)
5% SDS PAGE
All lanes: Western blot - Anti-Fibronectin antibody - N-terminal (ab137720) at 1/1000 dilution
All lanes: Hela whole cell lysate at 30 µg
Predicted band size: 262 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody - N-terminal (ab137720)
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labelling Fibronectin with ab137720 at 1/200 dilution. Lower image is merged with DNA probe.
Competitive ELISA - Anti-Fibronectin antibody - N-terminal (ab137720)
Competitive ELISA detection of human fibronectin in plasma using ab137720. Recombinant or purified native Fibronectin protein was coated to an ELISA plate at concentration of 10 µg/ml. Human plasma was applied as the competitors and was serial diluted at 1, 20, 200, 2000, 20000, 200000X dilutions. The primary antibody concentration was diluted to 14 ng/mL. Rabbit IgG antibody (HRP) was diluted at 1:2,000 and used to detect the primary antibody.
Western blot - Anti-Fibronectin antibody - N-terminal (ab137720)
Samples were seperated by 5% SDS-PAGE.
All lanes: Western blot - Anti-Fibronectin antibody - N-terminal (ab137720) at 1/1000 dilution
Lane 1: Untreated (–) HepG2 whole cell extracts at 30 µg
Lane 2: Treated (+) HepG2 whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 272 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody - N-terminal (ab137720)
Paraffin-embedded hepatoma tissue stained for FN1 protein at cytosol with ab137720 at a 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min.
Western blot - Anti-Fibronectin antibody - N-terminal (ab137720)
Samples were seperated by 5% SDS-PAGE.
All lanes: Western blot - Anti-Fibronectin antibody - N-terminal (ab137720) at 1/1000 dilution
Lane 1: HeLa whole cell extracts at 30 µg
Lane 2: HepG2 whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 272 kDa
Immunoprecipitation - Anti-Fibronectin antibody - N-terminal (ab137720)
Samples were seperated by 5% SDS-PAGE.
All lanes: Immunoprecipitation - Anti-Fibronectin antibody - N-terminal (ab137720) at 1/1000 dilution
All lanes: HeLa whole cell extracts at 30 µg
SecondaryAll lanes: anti-rabbit IgG (HRP)
Predicted band size: 272 kDa
Western blot - Anti-Fibronectin antibody - N-terminal (ab137720)
Samples were seperated by 5% SDS-PAGE.
All lanes: Western blot - Anti-Fibronectin antibody - N-terminal (ab137720) at 1/1000 dilution
All lanes: Mouse plasma at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 272 kDa
Indirect ELISA - Anti-Fibronectin antibody - N-terminal (ab137720)
Indirect ELISA analysis was performed by coating plate with 100 µL of recombinant Fibronectin protein at concentration of 10 µg/mL. The coated protein is detected with ab137720 at ranging from 0.5 to 140 ng/mL.
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