AB137720

Anti-Fibronectin antibody - N-terminal

Name: Anti-Fibronectin antibody - N-terminal (ab137720) | Abcam
Brand: Abcam Limited
SKU: AB137720
Price: 400 USD
Availability: InStock
Rating: 4 (1 reviews)

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(1 Review)

(9 Publications)

Rabbit Polyclonal Fibronectin antibody. Suitable for cELISA, I-ELISA, WB, ICC/IF, IHC-P, IP and reacts with Recombinant fragment, Human, Mouse, Recombinant fragment - Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FN1 aa 350 to C-terminus.

View Alternative Names

FN, FN1, Fibronectin, Cold-insoluble globulin, CIG

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody - N-terminal (AB137720)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody - N-terminal (AB137720)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labelling Fibronectin with ab137720 at 1/200 dilution. Lower image is merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody - N-terminal (AB137720)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody - N-terminal (AB137720)

Paraffin-embedded hepatoma tissue stained for FN1 protein at cytosol with ab137720 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : (EDTA based, pH 8.0) buffer, 15min.

Competitive ELISA - Anti-Fibronectin antibody - N-terminal (AB137720)

cELISA

Supplier Data

Competitive ELISA - Anti-Fibronectin antibody - N-terminal (AB137720)

Competitive ELISA detection of human fibronectin in plasma using ab137720. Recombinant or purified native Fibronectin protein was coated to an ELISA plate at concentration of 10 µg/ml. Human plasma was applied as the competitors and was serial diluted at 1, 20, 200, 2000, 20000, 200000X dilutions. The primary antibody concentration was diluted to 14 ng/mL. Rabbit IgG antibody (HRP) was diluted at 1 : 2,000 and used to detect the primary antibody.

Supplier Data

Immunoprecipitation - Anti-Fibronectin antibody - N-terminal (AB137720)

Samples were separated by 5% SDS-PAGE.

All lanes:

Immunoprecipitation - Anti-Fibronectin antibody - N-terminal (ab137720) at 1/1000 dilution

All lanes:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

anti-rabbit IgG (HRP)

Predicted band size: 272 kDa

false

Supplier Data

Western blot - Anti-Fibronectin antibody - N-terminal (AB137720)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-Fibronectin antibody - N-terminal (ab137720) at 1/1000 dilution

Lane 1:

HeLa whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 272 kDa

false

Supplier Data

Western blot - Anti-Fibronectin antibody - N-terminal (AB137720)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-Fibronectin antibody - N-terminal (ab137720) at 1/1000 dilution

Lane 1:

Untreated (–) HepG2 whole cell extracts at 30 µg

Lane 2:

Treated (+) HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 272 kDa

false

Supplier Data

Western blot - Anti-Fibronectin antibody - N-terminal (AB137720)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-Fibronectin antibody - N-terminal (ab137720) at 1/1000 dilution

All lanes:

Mouse plasma at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 272 kDa

false

Indirect ELISA - Anti-Fibronectin antibody - N-terminal (AB137720)

I-ELISA

Supplier Data

Indirect ELISA - Anti-Fibronectin antibody - N-terminal (AB137720)

Indirect ELISA analysis was performed by coating plate with 100 µL of recombinant Fibronectin protein at concentration of 10 µg/mL. The coated protein is detected with ab137720 at ranging from 0.5 to 140 ng/mL.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IP, IHC-P, I-ELISA, cELISA

applications

Immunogen

Recombinant Fragment Protein within Human FN1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P02751

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "cELISA-species-checked": "guaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "" }, "Mouse": { "cELISA-species-checked": "guaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "cELISA-species-checked": "predicted", "cELISA-species-dilution-info": "", "cELISA-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "cELISA-species-checked": "predicted", "cELISA-species-dilution-info": "", "cELISA-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Recombinant fragment": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "1/1000 - 1/10000", "cELISA-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Recombinant fragment - Human": { "cELISA-species-checked": "notRecommended", "cELISA-species-dilution-info": "", "cELISA-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1/1000 - 1/10000", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed : 34089617).. Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5 : ITGB1 integrin receptor signaling.

See full target information FN1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:7940-7955 PubMed40860156

2025

Targeting the self-amplifying loop between ATF6 and SNAI1 to inhibit epithelial‒mesenchymal transition in fibrotic lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Wu,Yan Liu,Jiyuan Ma,Lingshan Lu,Liang Wang,Mengmei He,Zhuang Hao,Xiaomin Liu,Luning Zhang,Chao Zhao,Mengzhang Tao,Chao Zheng,Jian Zhou

Investigative ophthalmology & visual science 66:35 PubMed40408092

2025

PERK Regulates Epithelial-Mesenchymal Transition Through Autophagy and Lipid Metabolism in Lens Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoran Wang,Baoxin Chen,Jieping Chen,Mi Huang,Shan Huang

Stem cells translational medicine 14: PubMed40036306

2025

Exosomes containing miR-148a-3p derived from mesenchymal stem cells suppress epithelial-mesenchymal transition in lens epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyu Ma,Qihang Sun,Yijia Chen,Jinyan Li,Shuyi Chen,Lixia Luo

Nature communications 16:1932 PubMed39994201

2025

Anti-PD1 based precision induction therapy in unresectable stage III non-small cell lung cancer: a phase II umbrella clinical trial.

Applications

Unspecified application

Species

Unspecified reactive species

Chengxiang Yi,Dongliang Bian,Jue Wang,Shiqi Hu,Liangdong Sun,Yilv Yan,Suyu Wang,Ziyun Shen,Huansha Yu,Yong Yang,Yirui Zhou,Xiaogang Liu,Nan Song,Yuming Zhu,Deping Zhao,Gening Jiang,Liang Duan,Wenxin He,Dong Xie,Jie Dai,Lele Zhang,Peng Zhang

Investigative ophthalmology & visual science 64:12 PubMed38079167

2023

Lanosterol Synthase Prevents EMT During Lens Epithelial Fibrosis Via Regulating SREBP1.

Applications

Unspecified application

Species

Unspecified reactive species

Pengjuan Ma,Jingqi Huang,Baoxin Chen,Mi Huang,Lang Xiong,Jieping Chen,Shan Huang,Yizhi Liu

Cells 11: PubMed36010635

2022

Long Non-Coding RNA Prevents Lens Fibrosis through Maintaining Lens Epithelial Cell Phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Lang Xiong,Yan Sun,Jingqi Huang,Pengjuan Ma,Xiaoran Wang,Jiani Wang,Baoxin Chen,Jieping Chen,Mi Huang,Shan Huang,Yizhi Liu

Investigative ophthalmology & visual science 63:26 PubMed35758908

2022

TFEB-Mediated Lysosomal Restoration Alleviates High Glucose-Induced Cataracts Via Attenuating Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Sun,Xiaoran Wang,Baoxin Chen,Mi Huang,Pengjuan Ma,Lang Xiong,Jingqi Huang,Jieping Chen,Shan Huang,Yizhi Liu

Cell proliferation 53:e12911 PubMed32985730

2020

MiR-22-3p inhibits fibrotic cataract through inactivation of HDAC6 and increase of α-tubulin acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoran Wang,Liping Wang,Yan Sun,Baoxin Chen,Lang Xiong,Jieping Chen,Mi Huang,Jing Wu,Xuhua Tan,Yingfeng Zheng,Shan Huang,Yizhi Liu

Molecular medicine reports 20:2929-2935 PubMed31322252

2019

All‑trans‑retinoic acid modulates TGF‑β‑induced apoptosis, proliferation, migration and extracellular matrix synthesis of conjunctival fibroblasts by inhibiting PI3K/AKT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lingling Liang,Xiaomei Wang,Yajuan Zheng,Yang Liu

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Fibronectin antibody - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody - N-terminal (AB137720)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody - N-terminal (AB137720)

Competitive ELISA - Anti-Fibronectin antibody - N-terminal (AB137720)

Immunoprecipitation - Anti-Fibronectin antibody - N-terminal (AB137720)

Western blot - Anti-Fibronectin antibody - N-terminal (AB137720)

Western blot - Anti-Fibronectin antibody - N-terminal (AB137720)

Western blot - Anti-Fibronectin antibody - N-terminal (AB137720)

Indirect ELISA - Anti-Fibronectin antibody - N-terminal (AB137720)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (9)

Product promise