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Mouse Monoclonal Fibronectin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing FN1 protein.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [TV.1] (AB194395), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Cell preparation containing FN1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P02751

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species

Human

Dilution info

1/1 - 1/2

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617).AnastellinBinds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.

Alternative names

Recommended products

Mouse Monoclonal Fibronectin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing FN1 protein.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Cell preparation containing FN1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P02751
Clone number

TV.1

Purification technique

Affinity purification Protein G

Specificity

ab194395 reacts with the cellular as well as plasma form of Fibronectin.

Light chain type

kappa

Concentration
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Purification notes

Purified from Bioreactor Concentrate

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

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1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [TV.1] (ab194395), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [TV.1] (ab194395)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic adenocarcinoma tissue labelling Fibronectin with ab194395 at 2 μg/mL. Heat mediated antigen retrieval was performed using 10 mM Tris/1 mM EDTA buffer pH 9 for 45 minutes at 95°C followed by cooling at room temperature for 20 minutes. Primary incubation for 30 minutes at room temperature.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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