Anti-Fibulin 1 antibody [CL0337]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Fibulin 1 antibody [CL0337] (AB211536)

Immunocytochemistry/Immunofluorescence analysis of RT-4 cells labelling Fibulin 1 with ab211536 at 2 μg/ml showing vesicle staining in green. Nuclear probes visualized in blue.

• IHC

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Immunohistochemistry - Anti-Fibulin 1 antibody [CL0337] (AB211536)

Immunohistochemical analysis of Human placenta tissue labeling Fibulin 1 with ab211536 at 1/50 dilution.

• IHC

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Immunohistochemistry - Anti-Fibulin 1 antibody [CL0337] (AB211536)

Immunohistochemical analysis of Human breast cancer tissue labeling Fibulin 1 with ab211536 at 1/50 dilution.

• IHC

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Immunohistochemistry - Anti-Fibulin 1 antibody [CL0337] (AB211536)

Immunohistochemical analysis of Human prostate tissue labeling Fibulin 1 with ab211536 at 1/50 dilution.

• IHC

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Immunohistochemistry - Anti-Fibulin 1 antibody [CL0337] (AB211536)

Immunohistochemical analysis of Human testis tissue labeling Fibulin 1 with ab211536 at 1/50 dilution.

• IHC

Supplier Data

Immunohistochemistry - Anti-Fibulin 1 antibody [CL0337] (AB211536)

Immunohistochemical analysis of Human uterus tissue labeling Fibulin 1 with ab211536 at 1/50 dilution.

• WB

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Western blot - Anti-Fibulin 1 antibody [CL0337] (AB211536)

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Western blot - Anti-Fibulin 1 antibody [CL0337] (ab211536) at 1/500 dilution

All lanes:

Human plasma

Predicted band size: 77 kDa

false

• IHC

CiteAb

Immunohistochemistry - Anti-Fibulin 1 antibody [CL0337] (AB211536)

Immunohistochemistry-paraffin using Anti-Fibulin 1 antibody [CL0337], ab211536. Publication image from Bartoschek, M. et al., 2018, Nat Commun, 30514914. Legend direct from paper.

vCAF and mCAF marker genes can be used to trace back subpopulations in tissue sections. a Violin plots of selected vCAF differentially expressed genes in log2(RPKM + 1). Violin colors represent mean expression of each population. Genes were sorted based on gene ontology terms. b Immunohistochemistry (IHC) staining of desmin on MMTV-PyMT tumor sections (6 µm). Images were acquired from the leading edge and the tumor center. Yellow boxes (left) indicate 2x magnified area (right). c IF staining of Nidogen-2 (green) and CD31 (magenta) or PDGFRα (red) on MMTV-PyMT tumor sections (5 µm) from mice of age 8 weeks, 12 weeks, and 15 weeks (top to bottom). Nuclei were counterstained with DAPI. d Immunofluorescence (IF) staining of Nidogen-2 on human tumor tissue (5 µm). Nuclei were counterstained with DAPI. e Violin plots of selected mCAF differentially expressed genes in log2(RPKM + 1). f IHC staining of fibulin-1 and PDGFRα in MMTV-PyMT tumor sections (6 µm). Images were acquired from the leading edge and the tumor center. IHC staining of fibulin-1 (g) and PDGFRα (h) in human tumor tissue sections (6 µm). FACS-sort of MMTV-PyMT tumor (i) and mammary gland (j) tissue. Gating on single, living CD45−CD31−NG2−EPCAM− cells followed by gating on PDGFRα+ cells (blue box) or PDGFRα- cells (red box). k Violin plots of cell cycle gene expression in log2(RPKM + 1). l IF staining for Nidogen-2 (red) and Ki-67 (grey) on sections (5 µm) from PDGFRα-EGFP (green) reporter mice. Arrows indicate Nid2+Ki67+ cCAF. Scale bar : 50 µm