Rabbit Recombinant Monoclonal Fibulin-4 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.
Images
![Western blot - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (AB248111), expandable thumbnail](https://content.abcam.com/products/images/fibulin-4-antibody-epr6842-bsa-and-azide-free-ab248111--western-blot-img26220.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (AB248111)")
![Western blot - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (AB248111), expandable thumbnail](https://content.abcam.com/products/images/fibulin-4-antibody-epr6842-bsa-and-azide-free-ab248111--western-blot-img106385.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (AB248111)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (AB248111), expandable thumbnail](https://content.abcam.com/products/images/fibulin-4-antibody-epr6842-bsa-and-azide-free-ab248111--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57884.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (AB248111)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (AB248111), expandable thumbnail](https://content.abcam.com/products/images/fibulin-4-antibody-epr6842-bsa-and-azide-free-ab248111--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59935.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (AB248111)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Expected | Not recommended | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Target data
Function
Plays a crucial role in elastic fiber formation in tissue, and in the formation of ultrastructural connections between elastic laminae and smooth muscle cells in the aorta, therefore participates in terminal differentiation and maturation of smooth muscle cell (SMC) and in the mechanical properties and wall integrity maintenance of the aorta (PubMed:27339457). In addition, is involved in the control of collagen fibril assembly in tissue throught proteolytic activation of LOX leading to cross- linking of collagen and elastin (By similarity). Also promotes ELN coacervation and participates in the deposition of ELN coacervates on to microfibrils but also regulates ELN cross- linking through LOX interaction (PubMed:18973305, PubMed:19570982). Moreover adheres to the cells through heparin binding in a calcium-dependent manner and regulates vascularlar smooth muscle cells proliferation through angiotensin signaling (PubMed:23782690).
Alternative names
FBLN4, UNQ200/PRO226, EFEMP2, EGF-containing fibulin-like extracellular matrix protein 2, Fibulin-4, Protein UPH1, FIBL-4
Recommended products
Rabbit Recombinant Monoclonal Fibulin-4 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR684(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248111 is the carrier-free version of Anti-Fibulin-4 antibody [EPR684(2)] ab125073.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111), expandable thumbnail](https://content.abcam.com/products/images/fibulin-4-antibody-epr6842-bsa-and-azide-free-ab248111--western-blot-img26220.jpg "Western blot - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)")
Western blot - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)
This data was developed using Anti-Fibulin-4 antibody [EPR684(2)] ab125073, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Fibulin-4 antibody [EPR684(2)] (Anti-Fibulin-4 antibody [EPR684(2)] ab125073) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney) whole cell lysates at 20 µg
Lane 2: HepG2 (human hepatocellular carcinoma) whole cell lysates at 20 µg
Lane 3: Human fetal lung tissue lysates at 20 µg
Lane 4: Human colon cancer tissue lysates at 20 µg
Lane 5: Human placenta tissue lysates at 20 µg
Lane 6: Mouse placenta tissue lysates at 20 µg
Lane 7: Mouse lung tissue lysates at 20 µg
Lane 8: NIH/3T3 (mouse embryo) whole cell lysates at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 49 kDa
Observed band size: 55 kDa
Exposure time: 3min
![Western blot - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111), expandable thumbnail](https://content.abcam.com/products/images/fibulin-4-antibody-epr6842-bsa-and-azide-free-ab248111--western-blot-img106385.jpg "Western blot - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)")
Western blot - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)
This data was developed using Anti-Fibulin-4 antibody [EPR684(2)] ab125073, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Fibulin-4 antibody [EPR684(2)] (Anti-Fibulin-4 antibody [EPR684(2)] ab125073) at 1/1000 dilution
Lane 1: JAR cell lysate at 10 µg
Lane 2: SW480 cell lysate at 10 µg
Lane 3: Human fetal lung tissue lysate at 10 µg
Predicted band size: 49 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111), expandable thumbnail](https://content.abcam.com/products/images/fibulin-4-antibody-epr6842-bsa-and-azide-free-ab248111--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57884.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)
This data was developed using Anti-Fibulin-4 antibody [EPR684(2)] ab125073, the same antibody clone in a different buffer formulation.Anti-Fibulin-4 antibody [EPR684(2)] ab125073, at 1/250, staining Fibulin-4 in paraffin-embedded Human breast carcinoma tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111), expandable thumbnail](https://content.abcam.com/products/images/fibulin-4-antibody-epr6842-bsa-and-azide-free-ab248111--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59935.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (ab248111)
This data was developed using Anti-Fibulin-4 antibody [EPR684(2)] ab125073, the same antibody clone in a different buffer formulation.Anti-Fibulin-4 antibody [EPR684(2)] ab125073, at 1/250, staining Fibulin-4 in paraffin-embedded Human muscle tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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