AB248111

Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Fibulin-4 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

View Alternative Names

FBLN4, UNQ200/PRO226, EFEMP2, EGF-containing fibulin-like extracellular matrix protein 2, Fibulin-4, Protein UPH1, FIBL-4

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (AB248111)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (AB248111)

This data was developed using ab125073, the same antibody clone in a different buffer formulation.

ab125073, at 1/250, staining Fibulin-4 in paraffin-embedded Human breast carcinoma tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (AB248111)

This data was developed using ab125073, the same antibody clone in a different buffer formulation.

ab125073, at 1/250, staining Fibulin-4 in paraffin-embedded Human muscle tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (AB248111)

This data was developed using ab125073, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Fibulin-4 antibody [EPR684(2)] (<a href='/en-us/products/primary-antibodies/fibulin-4-antibody-epr6842-ab125073'>ab125073</a>) at 1/1000 dilution

Lane 1:

JAR cell lysate at 10 µg

Lane 2:

SW480 cell lysate at 10 µg

Lane 3:

Human fetal lung tissue lysate at 10 µg

Predicted band size: 49 kDa

false

Lab

Western blot - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (AB248111)

This data was developed using ab125073, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Fibulin-4 antibody [EPR684(2)] (<a href='/en-us/products/primary-antibodies/fibulin-4-antibody-epr6842-ab125073'>ab125073</a>) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney) whole cell lysates at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma) whole cell lysates at 20 µg

Lane 3:

Human fetal lung tissue lysates at 20 µg

Lane 4:

Human colon cancer tissue lysates at 20 µg

Lane 5:

Human placenta tissue lysates at 20 µg

Lane 6:

Mouse placenta tissue lysates at 20 µg

Lane 7:

Mouse lung tissue lysates at 20 µg

Lane 8:

NIH/3T3 (mouse embryo) whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 49 kDa

Observed band size: 55 kDa

false

Exposure time: 3min

Unconjugated

Anti-Fibulin-4 antibody [EPR684(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR684(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab248111 is the carrier-free version of ab125073.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a crucial role in elastic fiber formation in tissue, and in the formation of ultrastructural connections between elastic laminae and smooth muscle cells in the aorta, therefore participates in terminal differentiation and maturation of smooth muscle cell (SMC) and in the mechanical properties and wall integrity maintenance of the aorta (PubMed : 27339457). In addition, is involved in the control of collagen fibril assembly in tissue throught proteolytic activation of LOX leading to cross- linking of collagen and elastin (By similarity). Also promotes ELN coacervation and participates in the deposition of ELN coacervates on to microfibrils but also regulates ELN cross- linking through LOX interaction (PubMed : 18973305, PubMed : 19570982). Moreover adheres to the cells through heparin binding in a calcium-dependent manner and regulates vascularlar smooth muscle cells proliferation through angiotensin signaling (PubMed : 23782690).

See full target information EFEMP2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (AB248111)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (AB248111)

Western blot - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (AB248111)

Western blot - Anti-Fibulin-4 antibody [EPR684(2)] - BSA and Azide free (AB248111)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Product promise