AB66339

Anti-Fibulin 5 antibody [1G6A4]

Name: Anti-Fibulin 5 antibody [1G6A4] (ab66339) | Abcam
Brand: Abcam Limited
SKU: AB66339
Price: 450 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(19 Publications )

Mouse Monoclonal Fibulin 5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Recombinant fragment - Human, Human samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FBLN5 aa 200 to C-terminus.

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 5 antibody [1G6A4] (AB66339)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 5 antibody [1G6A4] (AB66339)

Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue showing cytoplasmic localisation with DAB staining using ab66337 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Fibulin 5 antibody [1G6A4] (AB66339)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Fibulin 5 antibody [1G6A4] (AB66339)

ICC/IF image of ab66339 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66339, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 5 antibody [1G6A4] (AB66339)

Immunohistochemical analysis of paraffin embedded human colon cancer tissue showing cytoplasmic localisation with DAB staining using ab66337 at 1/500 dilution.

AbReview39172****

Western blot - Anti-Fibulin 5 antibody [1G6A4] (AB66339)

All lanes:

Western blot - Anti-Fibulin 5 antibody [1G6A4] (ab66339) at 1/2000 dilution

All lanes:

Human vascular smooth muscle whole cell lysate at 20 µg

Secondary

All lanes:

HRP-conjugated Rabbit anti-mouse IgG polyclonal at 1/2000 dilution

Predicted band size: 50 kDa

Observed band size: 55 kDa

false

Exposure time: 1min

This image is courtesy of an anonymous Abreview

Unknown

Western blot - Anti-Fibulin 5 antibody [1G6A4] (AB66339)

All lanes:

Western blot - Anti-Fibulin 5 antibody [1G6A4] (ab66339) at 1/500 dilution

All lanes:

Immunogen (recombinant truncated protein) at 1 µg

Predicted band size: 50 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1G6A4

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human FBLN5 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UBX5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

This product was changed from ascites to supernatant. Lot no's high than GR171104-21 are from Tissue Culture Supernatant

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for elastic fiber formation, is involved in the assembly of continuous elastin (ELN) polymer and promotes the interaction of microfibrils and ELN (PubMed : 18185537). Stabilizes and organizes elastic fibers in the skin, lung and vasculature (By similarity). Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Vascular ligand for integrin receptors which may play a role in vascular development and remodeling (PubMed : 10428823). May act as an adapter that mediates the interaction between FBN1 and ELN (PubMed : 17255108).

See full target information FBLN5

Alternative Names

DANCE, UNQ184/PRO210, FBLN5, Fibulin-5, FIBL-5, Developmental arteries and neural crest EGF-like protein, Urine p50 protein, Dance, UP50

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:7578-7595 PubMed38568089

2024

Establishment and verification of a prognostic model based on coagulation and fibrinolysis-related genes in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Fan,Le Lu,Hao Shang,Yuxuan Lu,Yi Yang,Xiuyan Wang,Hongwei Lu

British journal of cancer 129:1261-1273 PubMed37644092

2023

Loss of NDUFS1 promotes gastric cancer progression by activating the mitochondrial ROS-HIF1α-FBLN5 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Chen,Dongbao Li,Yunliang Wang,Xiaochun Shen,Anqi Dong,Chao Dong,Kaipeng Duan,Jiayu Ren,Weikang Li,Gege Shu,Jiaoyang Yang,Yufeng Xie,Fuliang Qian,Jin Zhou

Bioengineered 13:11867-11880 PubMed35543375

2022

Knockdown of ubiquitin-conjugating enzyme E2T (UBE2T) suppresses lung adenocarcinoma progression via targeting fibulin-5 (FBLN5).

Applications

Unspecified application

Species

Unspecified reactive species

Yi Li,Xiaojuan Yang,Dan Lu

Cells 10: PubMed34943956

2021

Primary Human Trabecular Meshwork Model for Pseudoexfoliation.

Applications

Unspecified application

Species

Unspecified reactive species

Munmun Chakraborty,Prity Sahay,Aparna Rao

Experimental and therapeutic medicine 22:896 PubMed34257709

2021

Effect of fibulin-5 on aldosterone-induced apoptosis in human ascending aortic smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yongji Yan,Xujie Liu,Xuanchen Li,Xuan Zhou,Chaozhi Yang,Jintao Tian,Jun Pu,Xiaoqun Niu

Journal of materials chemistry. B 8:9239-9250 PubMed32966543

2020

Insights into the biophysical forces between proteins involved in elastic fiber assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Sean O'Neill Moore,Tyler Jacob Grubb,Chandrasekhar R Kothapalli

Oncology reports 44:2143-2151 PubMed32901854

2020

FBLN5 is targeted by microRNA‑27a‑3p and suppresses tumorigenesis and progression in high‑grade serous ovarian carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rongrong Li,Huan Wu,Huiyang Jiang,Qiuman Wang,Zhiyuan Dou,Hanlin Ma,Shi Yan,Cunzhong Yuan,Ning Yang,Beihua Kong

European journal of human genetics : EJHG 27:1858-1866 PubMed31358954

2019

De novo variants in an extracellular matrix protein coding gene, fibulin-5 (FBLN5) are associated with pseudoexfoliation.

Applications

Unspecified application

Species

Unspecified reactive species

Biswajit Padhy,Ramani Shyam Kapuganti,Bushra Hayat,Pranjya Paramita Mohanty,Debasmita Pankaj Alone

Journal of cellular biochemistry 120:17838-17846 PubMed31148262

2019

Fibulin-5 contributes to colorectal cancer cell apoptosis via the ROS/MAPK and Akt signal pathways by downregulating transient receptor potential cation channel subfamily V member 1.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Chen,Junzhi Li,Lei Jin,Kai Lei,Haiwang Liu,Yong Yang

International urogynecology journal 30:439-446 PubMed29600404

2018

Differential gene expression of extracellular-matrix-related proteins in the vaginal apical compartment of women with pelvic organ prolapse.

Applications

Unspecified application

Species

Unspecified reactive species

Haijiao Wang,Yukimi Kira,Akihiro Hamuro,Aki Takase,Daisuke Tachibana,Masayasu Koyama

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fibulin 5 antibody [1G6A4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 5 antibody [1G6A4] (AB66339)

Immunocytochemistry/ Immunofluorescence - Anti-Fibulin 5 antibody [1G6A4] (AB66339)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 5 antibody [1G6A4] (AB66339)

Western blot - Anti-Fibulin 5 antibody [1G6A4] (AB66339)

Western blot - Anti-Fibulin 5 antibody [1G6A4] (AB66339)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Alternative Names

Publications (19)

Product promise