AB109428

Anti-Fibulin 5 antibody [EPR4506]

RabMAb
Recombinant
What is this?

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(8 Publications)

Rabbit Recombinant Monoclonal Fibulin 5 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 8 publications.

View Alternative Names

DANCE, UNQ184/PRO210, FBLN5, Fibulin-5, FIBL-5, Developmental arteries and neural crest EGF-like protein, Urine p50 protein, Dance, UP50

3 Images

Lab

Western blot - Anti-Fibulin 5 antibody [EPR4506] (AB109428)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Fibulin 5 antibody [EPR4506] (ab109428) at 1/1000 dilution

Lane 1:

Human fetal kidney tissue lysate at 20 µg

Lane 2:

Human fetal spleen tissue lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/5000 dilution

Predicted band size: 50 kDa

Observed band size: 50-60 kDa

false

Lab

Western blot - Anti-Fibulin 5 antibody [EPR4506] (AB109428)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Fibulin 5 antibody [EPR4506] (ab109428) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 21 kDa,26 kDa,48 kDa,50 kDa,51 kDa

Observed band size: 21 kDa,45 kDa,48 kDa,50-60 kDa

false

Unknown

Western blot - Anti-Fibulin 5 antibody [EPR4506] (AB109428)

All lanes:

Western blot - Anti-Fibulin 5 antibody [EPR4506] (ab109428) at 1/1000 dilution

Lane 1:

Fetal lung lysate at 10 µg

Lane 2:

Fetal kidney lysate at 10 µg

Lane 3:

Human testis lysate at 10 µg

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4506

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for elastic fiber formation, is involved in the assembly of continuous elastin (ELN) polymer and promotes the interaction of microfibrils and ELN (PubMed : 18185537). Stabilizes and organizes elastic fibers in the skin, lung and vasculature (By similarity). Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Vascular ligand for integrin receptors which may play a role in vascular development and remodeling (PubMed : 10428823). May act as an adapter that mediates the interaction between FBN1 and ELN (PubMed : 17255108).

See full target information FBLN5

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Food science & nutrition 12:1082-1094 PubMed38370085

2024

Anti-skin aging effect of sea buckthorn proanthocyanidins in D-galactose-induced aging mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xinying Liu,Michael Yuen,Tina Yuen,Hywel Yuen,Min Wang,Qiang Peng

Pharmacogenomics and personalized medicine 17:27-40 PubMed38264064

2024

FBLN5 as One Presumably Prognostic Gene Potentially Modulating Tumor Immune Microenvironment for Renal Clear Cell Carcinoma in Children and Young Adults.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Zhang,Feng Chen,Shaoguang Feng,Xu Liu,Zhen Wang,Nan Shen,Lingjian Meng,Dongsheng Zhu,Zhitao Zhu

Discover. Oncology 13:114 PubMed36306007

2022

LINC01089 blocks malignant progression of thyroid cancer by binding miR-27b-3p to enhance the FBLN5 protein level.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Qin Pan,Kun-Song Huang,Tsz-Hong Chong,Jin-Yi Li

Experimental and therapeutic medicine 23:407 PubMed35619637

2022

YY1 affects the levels and function of fibulin-5 in ox-LDL-treated vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Leijing Wang,Cuiyan Li,Chunli Feng,Yuanbo Zang

Frontiers in molecular biosciences 9:877250 PubMed35517867

2022

Global and Comparative Proteome Signatures in the Lens Capsule, Trabecular Meshwork, and Iris of Patients With Pseudoexfoliation Glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Prity Sahay,Munmun Chakraborty,Aparna Rao

The Journal of clinical investigation 131: PubMed34779414

2021

Candesartan prevents arteriopathy progression in cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy model.

Applications

Unspecified application

Species

Unspecified reactive species

Taisuke Kato,Ri-Ichiroh Manabe,Hironaka Igarashi,Fuyuki Kametani,Sachiko Hirokawa,Yumi Sekine,Natsumi Fujita,Satoshi Saito,Yusuke Kawashima,Yuya Hatano,Shoichiro Ando,Hiroaki Nozaki,Akihiro Sugai,Masahiro Uemura,Masaki Fukunaga,Toshiya Sato,Akihide Koyama,Rie Saito,Atsushi Sugie,Yasuko Toyoshima,Hirotoshi Kawata,Shigeo Murayama,Masaki Matsumoto,Akiyoshi Kakita,Masato Hasegawa,Masafumi Ihara,Masato Kanazawa,Masatoyo Nishizawa,Shoji Tsuji,Osamu Onodera

Breast cancer research : BCR 23:90 PubMed34565423

2021

A matrisome RNA signature from early-pregnancy mouse mammary fibroblasts predicts distant metastasis-free breast cancer survival in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Ayman M Ibrahim,Alan Bilsland,Steffen Rickelt,Joanna S Morris,Torsten Stein

The American journal of pathology 186:1786-1800 PubMed27181406

2016

Epithelial-Derived Inflammation Disrupts Elastin Assembly and Alters Saccular Stage Lung Development.

Applications

Unspecified application

Species

Unspecified reactive species

John T Benjamin,Riet van der Meer,Amanda M Im,Erin J Plosa,Rinat Zaynagetdinov,Ankita Burman,Madeline E Havrilla,Linda A Gleaves,Vasiliy V Polosukhin,Gail H Deutsch,Hiromi Yanagisawa,Jeffrey M Davidson,Lawrence S Prince,Lisa R Young,Timothy S Blackwell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fibulin 5 antibody [EPR4506]

Western blot - Anti-Fibulin 5 antibody [EPR4506] (AB109428)

Western blot - Anti-Fibulin 5 antibody [EPR4506] (AB109428)

Western blot - Anti-Fibulin 5 antibody [EPR4506] (AB109428)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (8)

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Product promise