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Rabbit Recombinant Monoclonal Fibulin 5 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 5 publications.

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Images

Western blot - Anti-Fibulin 5 antibody [EPR4506] (AB109428), expandable thumbnail
  • Western blot - Anti-Fibulin 5 antibody [EPR4506] (AB109428), expandable thumbnail
  • Western blot - Anti-Fibulin 5 antibody [EPR4506] (AB109428), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Essential for elastic fiber formation, is involved in the assembly of continuous elastin (ELN) polymer and promotes the interaction of microfibrils and ELN (PubMed:18185537). Stabilizes and organizes elastic fibers in the skin, lung and vasculature (By similarity). Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Vascular ligand for integrin receptors which may play a role in vascular development and remodeling (PubMed:10428823). May act as an adapter that mediates the interaction between FBN1 and ELN (PubMed:17255108).

Alternative names

DANCE, UNQ184/PRO210, FBLN5, Fibulin-5, FIBL-5, Developmental arteries and neural crest EGF-like protein, Urine p50 protein, Dance, UP50

Recommended products

Rabbit Recombinant Monoclonal Fibulin 5 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4506
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Fibulin 5 antibody [EPR4506] (ab109428), expandable thumbnail

    Western blot - Anti-Fibulin 5 antibody [EPR4506] (ab109428)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Fibulin 5 antibody [EPR4506] (ab109428) at 1/1000 dilution

    Lane 1: Human fetal kidney tissue lysate at 20 µg

    Lane 2: Human fetal spleen tissue lysate at 20 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/5000 dilution

    Predicted band size: 50 kDa

    Observed band size: 50-60 kDa

  • Western blot - Anti-Fibulin 5 antibody [EPR4506] (ab109428), expandable thumbnail

    Western blot - Anti-Fibulin 5 antibody [EPR4506] (ab109428)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Fibulin 5 antibody [EPR4506] (ab109428) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Lane 3: Mouse kidney tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Predicted band size: 21 kDa, 26 kDa, 48 kDa, 50 kDa, 51 kDa

    Observed band size: 21 kDa, 45 kDa, 48 kDa, 50-60 kDa

  • Western blot - Anti-Fibulin 5 antibody [EPR4506] (ab109428), expandable thumbnail

    Western blot - Anti-Fibulin 5 antibody [EPR4506] (ab109428)

    All lanes: Western blot - Anti-Fibulin 5 antibody [EPR4506] (ab109428) at 1/1000 dilution

    Lane 1: Fetal lung lysate at 10 µg

    Lane 2: Fetal kidney lysate at 10 µg

    Lane 3: Human testis lysate at 10 µg

    Predicted band size: 50 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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