Anti-Ficolin-3 antibody [EPR30745-69]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ficolin-3 antibody [EPR30745-69] (AB324673)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Ficolin-3 with ab324673 at 1/2000 (0.242 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human lung.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ficolin-3 antibody [EPR30745-69] (AB324673)

Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Ficolin-3 with ab324673 at 1/2000 (0.242 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on plasma in human endometrium.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ficolin-3 antibody [EPR30745-69] (AB324673)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ficolin-3 with ab324673 at 1/2000 (0.242 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human liver.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Ficolin-3 antibody [EPR30745-69] (AB324673)

Blocking and diluting buffer and concentration : QD20250827IMG36.

Low expression : testis, spleen, pancreas (PMID : 18006063).

The identity of the lower MW band at approximately 26 kDa (in lane 1) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-2 : 10 seconds; Lane 3 : 3 seconds; Lanes 4-6 : 10 seconds.

All lanes:

Western blot - Anti-Ficolin-3 antibody [EPR30745-69] (ab324673) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human testis tissue lysate  at 20 µg

Lane 3:

Human plasma at 20 µg

Lane 4:

Human lung tissue lysate at 20 µg

Lane 5:

Human spleen tissue lysate at 20 µg

Lane 6:

Human pancreas tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 32 kDa,36 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ficolin-3 antibody [EPR30745-69] (AB324673)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Ficolin-3 with ab324673 at 1/2000 (0.242 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on human skeletal muscle (PMID : 10330454).
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ficolin-3 antibody [EPR30745-69] (AB324673)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Ficolin-3 with ab324673 at 1/2000 (0.242 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : Nearly no staining on human tetsis (PMID : 18006063).
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Ficolin-3 antibody [EPR30745-69] (AB324673)

Blocking and diluting buffer and concentration : QD20250827IMG37.

This antibody does not cross-react with recombinant human FCN2 or FCN1 by western blot.

In Western blot, Anti-6X His tag® antibody [AD1.1.10]- (ab15149) staining at 1/1000 dilution.

Exposure time : Lane 1 : 3 seconds; Lanes 2-4 : 10 seconds.

All lanes:

Western blot - Anti-Ficolin-3 antibody [EPR30745-69] (ab324673) at 1/1000 dilution

Lane 1:

Human FCN3 recombinant protein (no-tag) at 10 ng

Lane 2:

His-tagged human FCN2 recombinant protein at 10 ng

Lane 3:

His-tagged human FCN1 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 25 kDa,26 kDa

false