JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB316950

Anti-FIG4 antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal FIG4 antibody. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human FIG4.

View Alternative Names

KIAA0274, SAC3, FIG4, Polyphosphoinositide phosphatase, SAC domain-containing protein 3, Serine-protein phosphatase FIG4

1 Images
Western blot - Anti-FIG4 Antibody (AB316950)
  • WB

Supplier Data

Western blot - Anti-FIG4 Antibody (AB316950)

Incubated with the primary antibody overnight at 4degC. Incubated with the secondary antibody at 25degC for 1 hour.

All lanes:

Western blot - Anti-FIG4 Antibody (ab316950) at 1/1000 dilution

Lane 1:

Mouse liver cells

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells

Lane 3:

Mouse brain cells

Secondary

All lanes:

Goat Anti-rabbit IgG IRDye 800 at 1/5000 dilution

Predicted band size: 103 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human FIG4.

Q92562

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C|-80°C
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FIG4 also known as SAC3 or Fleckstein1 is a phosphoinositide phosphatase enzyme with a mass of approximately 104 kDa. It is expressed in various tissues including the brain and muscle cells. Mechanically FIG4 functions to dephosphorylate phosphoinositides important molecules involved in membrane dynamics and intracellular signaling. By regulating the levels of these phosphoinositides FIG4 modulates cellular processes such as trafficking and autophagy contributing to proper cellular homeostasis.
Biological function summary

FIG4 engages in maintaining lysosomal function and endocytic membrane trafficking operating within a specific protein complex with VAC14 and PIKFYVE. This complex plays a role in the synthesis and turnover of phosphoinositide 35-bisphosphate (PI(35)P2) a lipid that modulates lysosome membrane stability and endosomal sorting. These activities are vital for cellular nutrient recycling and responding to cellular stress impacting cellular remodeling and health.

Pathways

FIG4 is involved in the phosphoinositide signaling pathway impacting the turnover of PI(35)P2. It connects with the autophagy pathway influencing lysosome biogenesis and function. Through these pathways FIG4 interacts with proteins like PIKFYVE and VAC14 forming a regulatory framework that ensures proper lysosomal degradation and membrane-related signaling required for cellular adaptation and response to environmental changes.

FIG4 mutations or dysregulation link to conditions such as Charcot-Marie-Tooth disease type 4J and amyotrophic lateral sclerosis (ALS). In these disorders altered levels of PI(35)P2 lead to abnormal lipid metabolism and cellular transport affecting neuron function and survival. The interactions with proteins like PIKFYVE and other phosphatases emphasize FIG4's role in maintaining neuronal integrity and its potential involvement in neurodegenerative disease progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Dual specificity phosphatase component of the PI(3,5)P2 regulatory complex which regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PubMed : 17556371, PubMed : 33098764). Catalyzes the dephosphorylation of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) to form phosphatidylinositol 3-phosphate (PubMed : 33098764). Has serine-protein phosphatase activity acting on PIKfyve to stimulate its lipid kinase activity, its catalytically activity being required for maximal PI(3,5)P2 production (PubMed : 33098764). In vitro, hydrolyzes all three D5-phosphorylated polyphosphoinositide and although displaying preferences for PtdIns(3,5)P2, it is capable of hydrolyzing PtdIns(3,4,5)P3 and PtdIns(4,5)P2, at least in vitro (PubMed : 17556371).
See full target information FIG4
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB224591

Anti-ESM1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ESM1 antibody (AB224591)

  • 0
  • 0
Primary Antibodies

AB187027

Anti-RhoA antibody [EPR18134]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-RhoA antibody [EPR18134] (AB187027)

  • 4
  • 1
Primary Antibodies

AB168387

Anti-Aquaporin 1 antibody [EPR11588(B)]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [EPR11588(B)] (AB168387)

  • 5
  • 4
Primary Antibodies

AB254726

Anti-ST3GAL2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST3GAL2 antibody (AB254726)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com