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AB232756

Anti-FIGN/Fidgetin antibody

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(3 Publications)

Rabbit Polyclonal FIGN/Fidgetin antibody. Suitable for WB, IHC-P and reacts with Human, Recombinant full length protein - Human, Pig samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FIGN aa 1-350.

View Alternative Names

Fidgetin, FIGN

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIGN/Fidgetin antibody (AB232756)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIGN/Fidgetin antibody (AB232756)

Formalin-fixed, paraffin-embedded human liver tissue stained for FIGN/Fidgetin using ab232756 at 20 ug/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIGN/Fidgetin antibody (AB232756)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIGN/Fidgetin antibody (AB232756)

Formalin-fixed, paraffin-embedded human kidney tissue stained for FIGN/Fidgetin using ab232756 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIGN/Fidgetin antibody (AB232756)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIGN/Fidgetin antibody (AB232756)

Formalin-fixed, paraffin-embedded human liver tissue stained for FIGN/Fidgetin using ab232756 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-FIGN/Fidgetin antibody (AB232756)
  • WB

Supplier Data

Western blot - Anti-FIGN/Fidgetin antibody (AB232756)

All lanes:

Western blot - Anti-FIGN/Fidgetin antibody (ab232756) at 3 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 2:

Pig kidney lysate

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution

Predicted band size: 82 kDa

false

Western blot - Anti-FIGN/Fidgetin antibody (AB232756)
  • WB

Supplier Data

Western blot - Anti-FIGN/Fidgetin antibody (AB232756)

All lanes:

Western blot - Anti-FIGN/Fidgetin antibody (ab232756) at 3 µg/mL

All lanes:

Recombinant human FIGN/Fidgetin protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution

Predicted band size: 82 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human FIGN aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

Q5HY92

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fidgetin also known as FIGN is a protein that plays a role in microtubule dynamics. It belongs to the AAA ATPase family and has a molecular mass of approximately 88 kDa. Fidgetin is primarily expressed in the nervous system and various other tissues including the lung and liver. It seems to regulate microtubule severing which is important for various cellular processes such as mitosis and intracellular transport.
Biological function summary

Fidgetin modulates the cytoskeleton by remodeling microtubules which affects cell shape and motility. This protein acts as a part of the broader microtubule-severing complex influencing both cellular division and structural organization. Its activities impact how cells can respond to changes in their environment particularly in nerve and muscle cells where precise structural adjustments are essential.

Pathways

Fidgetin has important roles in the regulation of the cytoskeleton specifically within microtubule-associated pathways. Fidgetin-related pathways typically involve interactions with tubulin and katanin another protein to manage microtubule length and distribution. Microtubule severing by Fidgetin aligns closely with pathways controlling cell cycle progression and intracellular transport ensuring effective cell functioning.

Fidgetin's disruption can relate to neurological disorders due to its influence on nervous system structure. For instance abnormalities in Fidgetin activity may be linked to neurodegenerative diseases where microtubule integrity becomes compromised. Additionally cancer can feature altered Fidgetin expression where its interaction with proteins like katanin can affect abnormal cell division and invasion contributing to tumor progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-dependent microtubule severing protein. Severs microtubules along their length and depolymerizes their ends, primarily the minus-end, that may lead to the suppression of microtubule growth from and attachment to centrosomes. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome.
See full target information FIGN
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Neural regeneration research 18:2727-2732 PubMed37449637

2023

Fidgetin interacting with microtubule end binding protein EB3 affects axonal regrowth in spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Ma,Junpei Wang,Qifeng Tu,Weijuan Bo,Zunlu Hu,Run Zhuo,Ronghua Wu,Zhangji Dong,Liang Qiang,Yan Liu,Mei Liu

Antioxidants (Basel, Switzerland) 12: PubMed37107173

2023

SDNOR, a Novel Antioxidative lncRNA, Is Essential for Maintaining the Normal State and Function of Porcine Follicular Granulosa Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yangan Huo,Qiqi Li,Liu Yang,Xiaoxue Li,Chen Sun,Yang Liu,Honglin Liu,Zengxiang Pan,Qifa Li,Xing Du

Development (Cambridge, England) 149: PubMed35420127

2022

Suppression of microRNA 124-3p and microRNA 340-5p ameliorates retinoic acid-induced cleft palate in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Yoshioka,Akiko Suzuki,Chihiro Iwaya,Junichi Iwata
View all publications
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