AB70841

Anti-FIGNL1 antibody

Name: Anti-FIGNL1 antibody (ab70841) | Abcam
Brand: Abcam Limited
SKU: AB70841
Price: 520 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal FIGNL1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FIGNL1 aa 1-50.

View Alternative Names

Fidgetin-like protein 1, FIGNL1

2 Images

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Western blot - Anti-FIGNL1 antibody (AB70841)

All lanes:

Western blot - Anti-FIGNL1 antibody (ab70841) at 1 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Lane 4:

Whole cell lysate from 293T cells at 50 µg

Lane 5:

Whole cell lysate from NIH3T3 cells at 50 µg

Predicted band size: 74 kDa

Observed band size: 71 kDa

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Exposure time: 3min

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Immunoprecipitation - Anti-FIGNL1 antibody (AB70841)

Detection of Human FIGNL1 by Immunoprecipitation in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded), using ab70841 for IP at 3 µg/mg lysate. Subsequent WB detection was performed using 1 µg/mg ab70841 and chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-FIGNL1 antibody (ab70841)

Predicted band size: 74 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human FIGNL1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q6PIW4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FIGNL1 (Fidgetin-Like 1) is a member of ATPase family associated with various cellular processes. It bears a molecular mass of approximately 80 kDa. This protein showcases a unique functionality due to its capacity for microtubule severing and remodeling activities. FIGNL1 performs a role in regulating cytoskeletal dynamics which are critical during key cellular events. The expression of FIGNL1 occurs in various tissues with significant expression levels observed in the testes and ovary emphasizing its importance in reproduction.

Biological function summary

FIGNL1 functions in DNA repair and homologous recombination processes. It becomes part of a larger protein complex facilitating its involvement in maintaining genomic integrity. This connection to DNA repair pathways demonstrates its essential role in preventing genotoxic stress. FIGNL1 interacts with proteins such as RAD51 aiding in the accurate repair of DNA double-strand breaks which prevents the accumulation of potentially harmful genetic errors.

Pathways

FIGNL1 integrates into the DNA damage response and repair pathways. It plays an important part in the homologous recombination repair pathway ensuring chromosome stability during cell division. Related proteins like BRCA2 influence FIGNL1’s function within this pathway linking it to mechanisms that preserve cellular genomic stability. The presence of these interactions is significant for a well-functioning cellular response to DNA damage.

FIGNL1's malfunction or inadequacy may contribute to cancer and infertility issues. Research indicates its association with breast cancer in which its pathway partners like BRCA2 become pivotal for understanding tumorigenesis. Additionally disruptions in its regulation can cause infertility highlighting its necessity in reproductive health. Through these pathways FIGNL1 connects to broader clinical concerns suggesting that its proper expression and function are critical for preventing disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Involved in DNA double-strand break (DBS) repair via homologous recombination (HR). Recruited at DSB sites independently of BRCA2, RAD51 and RAD51 paralogs in a H2AX-dependent manner. May regulate osteoblast proliferation and differentiation (PubMed : 23754376). May play a role in the control of male meiosis dynamic (By similarity).

See full target information FIGNL1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular biosciences 7:199 PubMed33015132

2020

Glutamate-Cysteine Ligase Catalytic Subunit Attenuated Hepatitis C Virus-Related Liver Fibrosis and Suppressed Endoplasmic Reticulum Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Na Fu,Dongdong Li,Wencong Li,Wen Zhao,Siyu Zhang,Lingdi Liu,Suxian Zhao,Jinghua Du,Lingbo Kong,Rongqi Wang,Yuguo Zhang,Yuemin Nan

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FIGNL1 antibody

Western blot - Anti-FIGNL1 antibody (AB70841)

Immunoprecipitation - Anti-FIGNL1 antibody (AB70841)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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