AB81468

Anti-Filaggrin antibody

Name: Anti-Filaggrin antibody (ab81468) | Abcam
Brand: Abcam Limited
SKU: AB81468
Price: 515 USD
Availability: InStock
Rating: 4 (9 reviews)

(9 Reviews)

(41 Publications)

Anti-Filaggrin antibody (ab81468) is a rabbit polyclonal antibody detecting Filaggrin in ICC/IF. Suitable for Human.

- Over 30 publications
- Trusted since 2009

View Alternative Names

Filaggrin, FLG

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Filaggrin antibody (AB81468)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Filaggrin antibody (AB81468)

ICC/IF image of ab81468 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81468, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human FLG. The exact immunogen used to generate this antibody is proprietary information.

P20930

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-Filaggrin antibody (ab81468) is a rabbit polyclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Trusted by the scientific community
Anti-Filaggrin (ab81468) was first used in a scientific publication in 2009 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-Filaggrin (ab81468) has over 5 independent reviews from customers.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.

See full target information FLG

style

left-column

Publications (41)

Recent publications for all applications. Explore the full list and refine your search

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 33:e70082 PubMed40880422

2025

Re-Epithelialisation in a Yorkshire Pig Full-Thickness Excisional Wound Model Is Associated With Keratinocyte Activation, Oxidative Stress, and Biomacromolecule Oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Dylan Tinney,John T Walker,Emily Truscott,Douglas W Hamilton

Frontiers in immunology 16:1503007 PubMed40491919

2025

Rab7 inhibitor enhances stem cell differentiation into keratinocyte-like cells with anti-inflammatory properties.

Applications

Unspecified application

Species

Unspecified reactive species

Raghad Alghazali,Mouna Tabebi,Moustafa Elmasry,Ahmed El-Serafi

Journal of ginseng research 49:326-330 PubMed40453350

2025

Compound K suppresses epidermal aging induced by IL-17A treatment and UVB irradiation.

Applications

Unspecified application

Species

Unspecified reactive species

A Young Kim,Ji-Yong Jung,Donghyun Kim,Jeong Hun Cho,Yong Deog Hong,Hyoung-June Kim,Si-Young Cho

Clinical, cosmetic and investigational dermatology 18:1151-1162 PubMed40356846

2025

-Derived Exosomes Enhance Skin Barrier Integrity by Upregulating Key Barrier-Related Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Han Cho,Ji-Woo Kim,Nari Kim,Hee-Sik Kim,Jun-Hwan Jang,Jun-Tae Bae,Wanil Kim

International journal of molecular sciences 26: PubMed40076477

2025

Bulge-Derived Epithelial Cells Isolated from Human Hair Follicles Using Enzymatic Digestion or Explants Result in Comparable Tissue-Engineered Skin.

Applications

Unspecified application

Species

Unspecified reactive species

Bettina Cattier,Rina Guignard,Israël Martel,Christian Martel,Carolyne Simard-Bisson,Danielle Larouche,Béatrice Guiraud,Sandrine Bessou-Touya,Lucie Germain

Scientific reports 13:22357 PubMed38102220

2023

β-endorphin suppresses ultraviolet B irradiation-induced epidermal barrier damage by regulating inflammation-dependent mTORC1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hyung-Su Kim,Hyoung-June Kim,Yong-Deog Hong,Eui Dong Son,Si-Young Cho

Nature communications 14:7660 PubMed37996412

2023

Vector-borne Trypanosoma brucei parasites develop in artificial human skin and persist as skin tissue forms.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Reuter,Laura Hauf,Fabian Imdahl,Rituparno Sen,Ehsan Vafadarnejad,Philipp Fey,Tamara Finger,Nicola G Jones,Heike Walles,Lars Barquist,Antoine-Emmanuel Saliba,Florian Groeber-Becker,Markus Engstler

Frontiers in immunology 14:1273182 PubMed38053996

2023

Epidermal keratinocyte-specific STAT3 deficiency aggravated atopic dermatitis-like skin inflammation in mice through TSLP upregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao-Yuan Wang,Yu-Xin Zheng,Fan Xu,Ying-Zhe Cui,Xue-Yan Chen,Si-Qi Chen,Bing-Xi Yan,Yuan Zhou,Min Zheng,Xiao-Yong Man

International journal of molecular sciences 24: PubMed38003573

2023

Lipids Reduce Cytokine-Induced Pro-Inflammatory Signalling and Barrier Dysfunction in Human Keratinocyte Models.

Applications

Unspecified application

Species

Unspecified reactive species

Jamie M L Kok,Georgina C Dowd,Jaydee D Cabral,Lyn M Wise

Journal of nanobiotechnology 21:354 PubMed37775761

2023

Bioinspired polydopamine nanoparticles as efficient antioxidative and anti-inflammatory enhancers against UV-induced skin damage.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Zhang,Yuqi Zhou,Zhaoting Jiang,Chenhui He,Bo Wang,Qi Wang,Zeqian Wang,Tong Wu,Xiaoqi Chen,Ziwei Deng,Chunying Li,Zhe Jian

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Filaggrin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Filaggrin antibody (AB81468)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (41)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise