Rabbit Polyclonal Filaggrin antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 35 publications. Immunogen corresponding to Synthetic Peptide within Human FLG.
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- Scientific reports 13:223572023β-endorphin suppresses ultraviolet B irradiation-induced epidermal barrier damage by regulating inflammation-dependent mTORC1 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyung-Su Kim,Hyoung-June Kim,Yong-Deog Hong,Eui Dong Son,Si-Young ChoPubMed 38102220
- Nature communications 14:76602023Vector-borne Trypanosoma brucei parasites develop in artificial human skin and persist as skin tissue forms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristian Reuter,Laura Hauf,Fabian Imdahl,Rituparno Sen,Ehsan Vafadarnejad,Philipp Fey,Tamara Finger,Nicola G Jones,Heike Walles,Lars Barquist,Antoine-Emmanuel Saliba,Florian Groeber-Becker,Markus EngstlerPubMed 37996412
- Frontiers in immunology 14:12731822023Epidermal keratinocyte-specific STAT3 deficiency aggravated atopic dermatitis-like skin inflammation in mice through TSLP upregulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhao-Yuan Wang,Yu-Xin Zheng,Fan Xu,Ying-Zhe Cui,Xue-Yan Chen,Si-Qi Chen,Bing-Xi Yan,Yuan Zhou,Min Zheng,Xiao-Yong ManPubMed 38053996
- International journal of molecular sciences 24:2023Lipids Reduce Cytokine-Induced Pro-Inflammatory Signalling and Barrier Dysfunction in Human Keratinocyte Models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJamie M L Kok,Georgina C Dowd,Jaydee D Cabral,Lyn M WisePubMed 38003573
- Journal of nanobiotechnology 21:3542023Bioinspired polydopamine nanoparticles as efficient antioxidative and anti-inflammatory enhancers against UV-induced skin damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Zhang,Yuqi Zhou,Zhaoting Jiang,Chenhui He,Bo Wang,Qi Wang,Zeqian Wang,Tong Wu,Xiaoqi Chen,Ziwei Deng,Chunying Li,Zhe JianPubMed 37775761
- International journal of molecular sciences 24:2023Epicutaneous Sensitization to the Phytocannabinoid β-Caryophyllene Induces Pruritic Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaadet Inan,Sara J Ward,Citlalli T Baltazar,Gabrielle A Peruggia,Elham Javed,Ajay P NayakPubMed 37762646
- International journal of bioprinting 9:7382023Developing a bioink for single-step deposition and maturation of human epidermis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuth Jinfen Chai,Wan Ling Wong,Cyrus Weijie BehPubMed 37323493
- STAR protocols 3:1016132022Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGowher Ali,Essam M AbdelalimPubMed 35990735
- Cells 11:2022Alpha-Keratin, Keratin-Associated Proteins and Transglutaminase 1 Are Present in the Ortho- and Parakeratinized Epithelium of the Avian Tongue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKinga Skieresz-Szewczyk,Hanna Jackowiak,Marek SkrzypskiPubMed 35741029
- International journal of molecular sciences 22:2021Development of an Aged Full-Thickness Skin Model Using Flexible Skin-on-a-Chip Subjected to Mechanical Stimulus Reflecting the Circadian Rhythm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSubin Jeong,Jisue Kim,Hye Mi Jeon,Kyunghee Kim,Gun Yong SungPubMed 34884594
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human FLG. The exact immunogen used to generate this antibody is proprietary information. Database link P20930
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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13 products for Alternative Product
Target data
Function
Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
Alternative names
Filaggrin, FLG
Recommended products
Rabbit Polyclonal Filaggrin antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 35 publications. Immunogen corresponding to Synthetic Peptide within Human FLG.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human FLG. The exact immunogen used to generate this antibody is proprietary information. Database link P20930
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 23rd June 2017. The following lots are still unpurified and still in stock as of 23rd June 2017 - GR309720-5, GR3178284-1, GR309720-6, GR309720-2, GR309720-3, GR309720-4. Lot numbers other than GR309720-5, GR3178284-1, GR309720-6, GR309720-2, GR309720-3, GR309720-4 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunocytochemistry/ Immunofluorescence - Anti-Filaggrin antibody (ab81468)
ICC/IF image of ab81468 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81468, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
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