Anti-Filaggrin antibody [EPR21892]

2 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filaggrin antibody [EPR21892] (ab221155)

  Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Filaggrin with ab221155 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in epidermis of human skin (PMID: 23152869) is observed. Counter stained with Hematoxylin.
  

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

  Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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  Multiplex immunohistochemistry - Anti-Filaggrin antibody [EPR21892] (ab221155)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue labelling Aquaporin 3 with Anti-Aquaporin 3 antibody [EPR28053-11] ab307969 at 1/5000 dilution (0.094 µg/mL) (B), Loricrin with Anti-Loricrin antibody [EPR7148(2)(B)] ab176322 at 1/3000 dilution (0.031 μg/ml) (C) and Filaggrin with ab221155 at 1/2000 dilution (0.523 μg/ml) (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
  
  Panel A: merged staining of anti-Aquaporin 3 (magenta; Opal™690), anti-Loricrin (green; Opal™520) and anti-Filaggrin (red; Opal™570) on human skin.
  Panel B: anti-Aquaporin 3 stained on keratinocytes from the stratum basale to the stratum granulosum of the epidermis.
  Panel C: anti-Loricrin stained on stratum corneum.
  Panel D: anti-Filaggrin stained on the granular to stratum corneum transition.
  
  The section was incubated in three rounds of staining: in the order of Anti-Aquaporin 3 antibody [EPR28053-11] ab307969, Anti-Loricrin antibody [EPR7148(2)(B)] ab176322, and ab221155 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.