Anti-Filamin A antibody [EP2405Y] - BSA and Azide free

4 product images

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  Flow Cytometry (Intracellular) - Anti-Filamin A antibody [EP2405Y] - BSA and Azide free (ab239881)

  Overlay histogram showing A431 cells stained with Anti-Filamin A antibody [EP2405Y] ab76289 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-Filamin A antibody [EP2405Y] ab76289 , 1/100 dilutio) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Filamin A antibody [EP2405Y] ab76289).
  

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A antibody [EP2405Y] - BSA and Azide free (ab239881)

  Immunohistochemical analysis of paraffin-embedded human uterus using Anti-Filamin A antibody [EP2405Y] ab76289 at a 1/100 dilution.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Filamin A antibody [EP2405Y] ab76289).

  Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

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  Immunocytochemistry/ Immunofluorescence - Anti-Filamin A antibody [EP2405Y] - BSA and Azide free (ab239881)

  Immunofluorescent staining of HeLa cells using Anti-Filamin A antibody [EP2405Y] ab76289 at a 1/100 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Filamin A antibody [EP2405Y] ab76289).

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  Flow Cytometry (Intracellular) - Anti-Filamin A antibody [EP2405Y] - BSA and Azide free (ab239881)

  Anti-Filamin A antibody [EP2405Y] ab76289 staining Filamin A in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor^® 488) at a dilution of 1/2000 was used as the secondary antibody.
  

  Isoytype control: Rabbit monoclonal IgG (Black)

  Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Filamin A antibody [EP2405Y] ab76289).