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AB68424

Anti-Filamin A (phospho S1458) antibody [EP2309Y]

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(1 Publication)

Rabbit Recombinant Monoclonal Filamin A phospho S1458 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

FLN, FLN1, FLNA, Filamin-A, FLN-A, Actin-binding protein 280, Alpha-filamin, Endothelial actin-binding protein, Filamin-1, Non-muscle filamin, ABP-280

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A (phospho S1458) antibody [EP2309Y] (AB68424)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A (phospho S1458) antibody [EP2309Y] (AB68424)

Positive staining on smooth muscle cells of human stomach without alkaline phosphatase protein phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase protein phosphatase (image B). The sections were using Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, then labeling ab68424 at 1/500 dilution for 30 mins at room temperature. Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A (phospho S1458) antibody [EP2309Y] (AB68424)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A (phospho S1458) antibody [EP2309Y] (AB68424)

Immunohistochemical analysis of paraffin-embedded human stomach (left) and human uterus tissue (right) using ab68424 at 1/10 dilution. Heat-mediated antigen retrieval was performed prior to commencing with IHC protocol and an HRP-conjugated polymerized secondary antibody was used.

Western blot - Anti-Filamin A (phospho S1458) antibody [EP2309Y] (AB68424)
  • WB

Supplier Data

Western blot - Anti-Filamin A (phospho S1458) antibody [EP2309Y] (AB68424)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

We are not sure how to define the extra band below the band of interest.

All lanes:

Western blot - Anti-Filamin A (phospho S1458) antibody [EP2309Y] (ab68424) at 1/20000 dilution

Lane 1:

Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

HeLa whole cell lysate, then the membrane was incubated with alkaline phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 281 kDa

Observed band size: 260 kDa

false

Exposure time: 60s

Western blot - Anti-Filamin A (phospho S1458) antibody [EP2309Y] (AB68424)
  • WB

Unknown

Western blot - Anti-Filamin A (phospho S1458) antibody [EP2309Y] (AB68424)

All lanes:

Western blot - Anti-Filamin A (phospho S1458) antibody [EP2309Y] (ab68424) at 1/5000 dilution

Lane 1:

HeLa cell lysate - untreated at 10 µg

Lane 2:

HeLa cell lysate - treated with AP at 10 µg

Secondary

All lanes:

HRP-labelled Goat anti-Rabbit antibody at 1/2000 dilution

Predicted band size: 281 kDa

Observed band size: 260 kDa

false

  • Carrier free

    Anti-Filamin A (phospho S1458) antibody [EP2309Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2309Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "<p></p>" } } }
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Product details

This product has switched from a hybridoma to recombinant production method on 8th August 2024.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking (By similarity). Involved in ciliogenesis. Plays a role in cell-cell contacts and adherens junctions during the development of blood vessels, heart and brain organs. Plays a role in platelets morphology through interaction with SYK that regulates ITAM- and ITAM-like-containing receptor signaling, resulting in by platelet cytoskeleton organization maintenance (By similarity). During the axon guidance process, required for growth cone collapse induced by SEMA3A-mediated stimulation of neurons (PubMed : 25358863).
See full target information FLNA pS1458
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biotechnology journal 19:e202400432 PubMed39655405

2024

A Competition-Based Strategy for the Isolation of an Anti-Idiotypic Blocking Module and Fine-Tuning for Conditional Activation of a Therapeutic Antibody.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Habermann,Dominic Happel,Adrian Bloch,Charles Shin,Harald Kolmar
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com