Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] - BSA and Azide free (AB284727)

This data was developed using ab75978 the same antibody clone in a different buffer. Immunohistochemistry analysis of Paraffin-embedded human kidney sections labeling Filamin A (phospho S2152) + Filamin B (phospho S2107) with ab75978 at 1/200 dilution followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Positive staining on human kidney without alkaline phosphatase treatment (image A); No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab75978 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] - BSA and Azide free (AB284727)

This data was developed using ab75978 the same antibody clone in a different buffer. Immunohistochemistry analysis of Paraffin-embedded rat kidney sections labeling Filamin A (phospho S2152) + Filamin B (phospho S2107) with ab75978 at 1/200 dilution followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Positive staining on rat kidney without alkaline phosphatase treatment (image A); No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab75978 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] - BSA and Azide free (AB284727)

This data was developed using ab75978 the same antibody clone in a different buffer. Immunohistochemistry analysis of Paraffin-embedded human cardiac muscle sections labeling Filamin A (phospho S2152) + Filamin B (phospho S2107) with ab75978 at 1/200 dilution followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Negative control : No staining on the intercalated disks in human cardiac muscle, which shows ab75978 doesn't react with Filamin C. The section was incubated with ab75978 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] - BSA and Azide free (AB284727)

This data was developed using ab75978 the same antibody clone in a different buffer. Immunohistochemistry analysis of Paraffin-embedded mouse kidney sections labeling Filamin A (phospho S2152) + Filamin B (phospho S2107) with ab75978 at 1/200 dilution followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Positive staining on mouse kidney without alkaline phosphatase treatment (image A); No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab75978 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• WB

Lab

Western blot - Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] - BSA and Azide free (AB284727)

This data was developed using ab75978 the same antibody clone in a different buffer. Blocking/Diluting buffer and concentration : 5% NFDM/TBST Exposure time 5.5 seconds

All lanes:

Western blot - Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] (<a href='/en-us/products/primary-antibodies/filamin-a-phospho-s2152-filamin-b-phospho-s2107-antibody-ep2310ay-ab75978'>ab75978</a>) at 1/500 dilution

Lane 1:

L-929 (mouse connective tissue fibroblast) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

L-929 (mouse connective tissue fibroblast) whole cell lysate (phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 280 kDa

false

• Dot

Lab

Dot Blot - Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] - BSA and Azide free (AB284727)

This data was developed using ab75978 the same antibody clone in a different buffer. Dot blot analysis of Filamin A (phospho S2152) + Filamin B (phospho S2107) using ab75978 at 1/1000 followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution. Lane 1 : Filamin A non-phospho peptide Lane 2 : Filamin A (phospho S2152) peptide a Lane 3 : Filamin A (phospho S2158) peptide b Lane 4 : Filamin B non-phospho peptide Lane 5 : Filamin B (phospho S2107) peptide a Lane 6 : Filamin B (phospho S2113) peptide b Lane 7 : Filamin C non-phospho peptide Lane 8 : Filamin C (phospho S2146) peptide a Lane 9 : Filamin C (phospho S2152) peptide b Exposure time : 180 seconds Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does cross-react with Filamin A (phospho S2152) and Filamin B (phospho S2107).