Anti-Filamin B antibody [EPR24525-3] (ab282106)

Rabbit Recombinant Monoclonal Filamin B antibody. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody [EPR24525-3] (AB282106), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC/IF	IP	WB	Flow Cyt (Intra)
Human	Tested	Tested	Not recommended	Tested	Tested
Mouse	Tested	Tested	Not recommended	Tested	Tested
Rat	Tested	Expected	Not recommended	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -
Species Human	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information FLNB

Function

Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro.

Alternative names

FLN1L, FLN3, TABP, TAP, FLNB, Filamin-B, FLN-B, ABP-278, ABP-280 homolog, Actin-binding-like protein, Beta-filamin, Filamin homolog 1, Filamin-3, Thyroid autoantigen, Truncated actin-binding protein, Fh1, Truncated ABP

Recommended products

Rabbit Recombinant Monoclonal Filamin B antibody. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR24525-3
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody [EPR24525-3] (ab282106)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labelling Filamin B with ab282106 at 1/5000 (0.103 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on Mouse colon. The section was incubated with ab282106 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunocytochemistry/ Immunofluorescence - Anti-Filamin B antibody [EPR24525-3] (ab282106)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-1080 cells labelling Filamin B with ab282106 at 1/50 (10.3 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluo^r® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoskeletal staining in HT-1080 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-Filamin B antibody [EPR24525-3] (ab282106)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HT-1080 (Human fibrosarcoma epithelial cell) cells labelling Filamin B with ab282106 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-Filamin B antibody [EPR24525-3] (ab282106)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 28175289, 11807098).
Lysates were made freshly and used in WB test immediately to minimize protein degradation.
Exposure time: Lanes 1-2: 15 seconds; lanes 3-4: 8 seconds.
All lanes: Western blot - Anti-Filamin B antibody [EPR24525-3] (ab282106) at 1/1000 dilution
Lane 1: A-204 (human muscle rhabdomyosarcoma) whole cell lysate at 20 µg
Lane 2: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 3: A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg
Lane 4: HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 278 kDa
Observed band size: 315 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody [EPR24525-3] (ab282106)
Immunohistochemical analysis of paraffin-embedded Human skin tissue labelling Filamin B with ab282106 at 1/5000 (0.103 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human skin. The section was incubated with ab282106 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunocytochemistry/ Immunofluorescence - Anti-Filamin B antibody [EPR24525-3] (ab282106)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 cells labelling Filamin B with ab282106 at 1/50 (10.3 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoskeletal staining in C2C12 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Western blot - Anti-Filamin B antibody [EPR24525-3] (ab282106)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 28175289, 11807098).
Low expression: skeletal muscle (PMID: 28175289, 11807098).
Exposure time: 26 seconds.
All lanes: Western blot - Anti-Filamin B antibody [EPR24525-3] (ab282106) at 1/1000 dilution
Lane 1: Human lung tissue lysate at 20 µg
Lane 2: Human heart tissue lysate at 20 µg
Lane 3: Human skeletal muscle tissue lysate at 20 µg
Lane 4: Mouse lung tissue lysate at 20 µg
Lane 5: Mouse skeletal muscle tissue lysate at 20 µg
Lane 6: Mouse brain tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 278 kDa
Observed band size: 315 kDa
Flow Cytometry (Intracellular) - Anti-Filamin B antibody [EPR24525-3] (ab282106)
Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) cells labelling Filamin B with ab282106 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-Filamin B antibody [EPR24525-3] (ab282106)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 28175289, 11807098).
Low expression: skeletal muscle (PMID: 28175289, 11807098).
Exposure time: 10 seconds.
All lanes: Western blot - Anti-Filamin B antibody [EPR24525-3] (ab282106) at 1/1000 dilution
Lane 1: Rat lung tissue lysate at 20 µg
Lane 2: Rat skeletal muscle tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 278 kDa
Observed band size: 315 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody [EPR24525-3] (ab282106)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Filamin B with ab282106 at 1/5000 (0.103 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human colon. (PMID: 32907537) The section was incubated with ab282106 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Western blot - Anti-Filamin B antibody [EPR24525-3] (ab282106)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 28175289, 11807098).
Exposure time: 3 seconds.
All lanes: Western blot - Anti-Filamin B antibody [EPR24525-3] (ab282106) at 1/1000 dilution
Lane 1: Mouse colon tissue lysate at 20 µg
Lane 2: Mouse stomach tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 278 kDa
Observed band size: 315 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody [EPR24525-3] (ab282106)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labelling Filamin B with ab282106 at 1/5000 (0.103 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat colon. The section was incubated with ab282106 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Flow Cytometry (Intracellular) - Anti-Filamin B antibody [EPR24525-3] (ab282106)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) cells labelling Filamin B with ab282106 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.