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AB126235

Anti-Filensin antibody

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(1 Publication)

Rabbit Polyclonal Filensin antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human BFSP1 aa 1-200.

View Alternative Names

Filensin, Beaded filament structural protein 1, Lens fiber cell beaded-filament structural protein CP 115, Lens intermediate filament-like heavy, CP115, LIFL-H, BFSP1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filensin antibody (AB126235)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filensin antibody (AB126235)

ab126235, at a 1/500 dilution, staining Filensin in paraffin embedded U373 xenograft by Immunohistochemistry.

Western blot - Anti-Filensin antibody (AB126235)
  • WB

Unknown

Western blot - Anti-Filensin antibody (AB126235)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Filensin antibody (ab126235) at 1/1000 dilution

All lanes:

HCT116 whole cell lysate at 30 µg

Predicted band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human BFSP1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q12934

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Suggested antigen retrieval: use heat mediated 10mM Citrate buffer(PH6.0) or Tris-EDTA buffer (PH 8.0).</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Filensin also known as beaded filament structural protein 1 (BFSP1) is a cytoskeletal intermediate filament protein. It plays a role in maintaining the structural integrity of cells. The molecular mass of Filensin is approximately 100 kDa. It expresses mainly in the lens fibers of the eye where it contributes to the unique architecture and function of the lens. In the lens Filensin interacts with other proteins to form a filament network important for maintaining lens transparency and flexibility.
Biological function summary

Filensin interacts with phakinin also called beaded filament structural protein 2 (BFSP2) forming a complex known as the beaded filament. This complex provides structural support to lens fiber cells essential for their long-term survival and functional maintenance. Filensin's role is vital for maintaining the mechanical properties and flexibility of lens cells ensuring the proper refractive function of the lens. Its expression and function are tightly regulated during lens development and differentiation.

Pathways

Filensin is part of the intermediate filament pathway influencing cell structure and mechanical stability. It associates with the cytoskeletal organization pathways playing a role in maintaining the shape and elasticity of lens fibers. Through these pathways Filensin interacts with proteins like vimentin and alpha-crystallin which are involved in maintaining lens cell integrity and preventing aggregation of lens proteins. These interactions are important for lens transparency and visual function.

Filensin mutations have been linked to cataracts a condition characterized by lens opacity leading to impaired vision. Mutations or dysfunctions in Filensin along with its partner phakinin disrupts the structural integrity of the lens causing cataract formation. Abnormalities in these proteins affect lens transparency and elasticity leading to progressive visual impairment. Studying these relationships provides insights into therapeutic targets for preventing or treating cataracts and related lens disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA (PubMed : 28935373). Involved in altering the calcium regulation of MIP water permeability (PubMed : 30790544).
See full target information BFSP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 12:1396890 PubMed38983788

2024

Screening of potentially active compounds against rheumatoid arthritis in the Juan-Bi decoction using systems pharmacology and animal experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Dahai Liu,Qiang Fu,Leyna G Liu,Wenwen Li,Fei Qi,Justin Liu,Lifeng Shang,Xiu Wang,Fang Yang,Jie Li,Daoqiang Lu,Huiying Feng,Ziwen Zhang,Yiqing Chen,Junru Liang,Jiayi Yao,Hua Lv,Riwang Li,Jun Wang,Di Wu,Yuxi Liu,Chenglai Xia,Wenxing Li
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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