Rabbit Polyclonal Filensin antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human BFSP1 aa 1-200.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human BFSP1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q12934
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Suggested antigen retrieval: use heat mediated 10mM Citrate buffer(PH6.0) or Tris-EDTA buffer (PH 8.0). |
Target data
Function
Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA (PubMed:28935373). Involved in altering the calcium regulation of MIP water permeability (PubMed:30790544).
Alternative names
Filensin, Beaded filament structural protein 1, Lens fiber cell beaded-filament structural protein CP 115, Lens intermediate filament-like heavy, CP115, LIFL-H, BFSP1
Recommended products
Rabbit Polyclonal Filensin antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human BFSP1 aa 1-200.
Alternative names
Filensin, Beaded filament structural protein 1, Lens fiber cell beaded-filament structural protein CP 115, Lens intermediate filament-like heavy, CP115, LIFL-H, BFSP1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human BFSP1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q12934
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Filensin also known as beaded filament structural protein 1 (BFSP1) is a cytoskeletal intermediate filament protein. It plays a role in maintaining the structural integrity of cells. The molecular mass of Filensin is approximately 100 kDa. It expresses mainly in the lens fibers of the eye where it contributes to the unique architecture and function of the lens. In the lens Filensin interacts with other proteins to form a filament network important for maintaining lens transparency and flexibility.
- Biological function summary
Filensin interacts with phakinin also called beaded filament structural protein 2 (BFSP2) forming a complex known as the beaded filament. This complex provides structural support to lens fiber cells essential for their long-term survival and functional maintenance. Filensin's role is vital for maintaining the mechanical properties and flexibility of lens cells ensuring the proper refractive function of the lens. Its expression and function are tightly regulated during lens development and differentiation.
- Pathways
Filensin is part of the intermediate filament pathway influencing cell structure and mechanical stability. It associates with the cytoskeletal organization pathways playing a role in maintaining the shape and elasticity of lens fibers. Through these pathways Filensin interacts with proteins like vimentin and alpha-crystallin which are involved in maintaining lens cell integrity and preventing aggregation of lens proteins. These interactions are important for lens transparency and visual function.
- Associated diseases and disorders
Filensin mutations have been linked to cataracts a condition characterized by lens opacity leading to impaired vision. Mutations or dysfunctions in Filensin along with its partner phakinin disrupts the structural integrity of the lens causing cataract formation. Abnormalities in these proteins affect lens transparency and elasticity leading to progressive visual impairment. Studying these relationships provides insights into therapeutic targets for preventing or treating cataracts and related lens disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Filensin antibody (ab126235)
7.5% SDS PAGE
All lanes: Western blot - Anti-Filensin antibody (ab126235) at 1/1000 dilution
All lanes: HCT116 whole cell lysate at 30 µg
Predicted band size: 75 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filensin antibody (ab126235)
ab126235, at a 1/500 dilution, staining Filensin in paraffin embedded U373 xenograft by Immunohistochemistry.
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