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AB251869

Anti-Filensin antibody

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(1 Publication)

Rabbit Polyclonal Filensin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human BFSP1 aa 250-350.

View Alternative Names

Filensin, Beaded filament structural protein 1, Lens fiber cell beaded-filament structural protein CP 115, Lens intermediate filament-like heavy, CP115, LIFL-H, BFSP1

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filensin antibody (AB251869)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filensin antibody (AB251869)

Paraffin-embedded human lymph node tissue stained for Filensin using ab251869 at 1/5000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filensin antibody (AB251869)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filensin antibody (AB251869)

Paraffin-embedded human eye tissue stained for Filensin using ab251869 at 1/5000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filensin antibody (AB251869)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filensin antibody (AB251869)

Paraffin-embedded human testis tissue stained for Filensin using ab251869 at 1/5000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Filensin antibody (AB251869)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Filensin antibody (AB251869)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Filensin (green) using ab251869 at 4 µg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filensin antibody (AB251869)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filensin antibody (AB251869)

Paraffin-embedded human kidney tissue stained for Filensin using ab251869 at 1/5000 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human BFSP1 aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

Q12934

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Filensin also known as beaded filament structural protein 1 (BFSP1) is a cytoskeletal intermediate filament protein. It plays a role in maintaining the structural integrity of cells. The molecular mass of Filensin is approximately 100 kDa. It expresses mainly in the lens fibers of the eye where it contributes to the unique architecture and function of the lens. In the lens Filensin interacts with other proteins to form a filament network important for maintaining lens transparency and flexibility.
Biological function summary

Filensin interacts with phakinin also called beaded filament structural protein 2 (BFSP2) forming a complex known as the beaded filament. This complex provides structural support to lens fiber cells essential for their long-term survival and functional maintenance. Filensin's role is vital for maintaining the mechanical properties and flexibility of lens cells ensuring the proper refractive function of the lens. Its expression and function are tightly regulated during lens development and differentiation.

Pathways

Filensin is part of the intermediate filament pathway influencing cell structure and mechanical stability. It associates with the cytoskeletal organization pathways playing a role in maintaining the shape and elasticity of lens fibers. Through these pathways Filensin interacts with proteins like vimentin and alpha-crystallin which are involved in maintaining lens cell integrity and preventing aggregation of lens proteins. These interactions are important for lens transparency and visual function.

Filensin mutations have been linked to cataracts a condition characterized by lens opacity leading to impaired vision. Mutations or dysfunctions in Filensin along with its partner phakinin disrupts the structural integrity of the lens causing cataract formation. Abnormalities in these proteins affect lens transparency and elasticity leading to progressive visual impairment. Studying these relationships provides insights into therapeutic targets for preventing or treating cataracts and related lens disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA (PubMed : 28935373). Involved in altering the calcium regulation of MIP water permeability (PubMed : 30790544).
See full target information BFSP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e35303 PubMed39170313

2024

ITPKA phosphorylates PYCR1 and promotes the progression of glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangying Luo,Tao Chen,Junyi Deng,Ziyuan Liu,Changlong Bi,Song Lan
View all publications
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