AB203874

Anti-FILGAP antibody

Name: Anti-FILGAP antibody (ab203874) | Abcam
Brand: Abcam Limited
SKU: AB203874

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(6 Publications)

Rabbit Polyclonal FILGAP antibody. Suitable for WB, IHC-P and reacts with Human, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human ARHGAP24 aa 600-700 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

FILGAP, ARHGAP24, Rho GTPase-activating protein 24, Filamin-A-associated RhoGAP, RAC1- and CDC42-specific GTPase-activating protein of 72 kDa, Rho-type GTPase-activating protein 24, RhoGAP of 73 kDa, Sarcoma antigen NY-SAR-88, p73RhoGAP, FilGAP, RC-GAP72

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FILGAP antibody (AB203874)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FILGAP antibody (AB203874)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling FILGAP with ab203874 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Supplier Data

Western blot - Anti-FILGAP antibody (AB203874)

All lanes:

Western blot - Anti-FILGAP antibody (ab203874) at 1/200 dilution

Lane 1:

Human colon carcinoma lysate

Lane 2:

Rat kidney lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution

Predicted band size: 84 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ARHGAP24 aa 600-700 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q8N264

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FILGAP also known as ARHGAP24 is an important regulator in the Rho family of GTPases. This protein with a mass of approximately 67 kDa serves as a GTPase-activating protein (GAP) specific for Rac1. Through its action FILGAP inhibits Rac1-mediated signals which control actin dynamics. Expression of FILGAP occurs in various tissue types with significant levels in cells involved in cytoskeletal arrangement and cell movement such as fibroblasts and endothelial cells.

Biological function summary

FILGAP influences the rearrangement of actin cytoskeleton by inactivating Rac1 which is an essential factor for lamellipodia formation and cell migration. FILGAP interacts physically with the protein Filamin A suggesting its involvement in forming a protein complex that modulates actin cytoskeleton assembly. This interaction positions FILGAP at intricate cellular junctions where mechanical stimuli affect cell structure and signaling.

Pathways

The function of FILGAP fits largely within the Rho GTPase signaling pathway and the regulation of adherens junctions. It has interactions with other proteins including but not limited to Rac1 and Cdc42 linking it to pathways controlling cell migration and adhesion. Through its regulation of Rac1 FILGAP coordinates with pathways that affect cellular morphogenesis and mechanotransduction.

Aberrant expression or function of FILGAP links to cancer progression where its regulatory role impacts cell migration and invasion capabilities. Additionally disruptions in Rho GTPase pathways involving FILGAP contribute to cardiovascular disorders such as atherosclerosis due to its role in endothelial cell movement. In both contexts its interaction with Filamin A becomes significant influencing disease mechanisms tied to cytoskeletal abnormalities and cell motility.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis.

See full target information ARHGAP24

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 299:102820 PubMed36549646

2022

Smad2Δexon3 and Smad3 have distinct properties in signal transmission leading to TGF-β-induced cell motility.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Yokoyama,Takahito Kuga,Yuka Itoh,Shigeo Otake,Chiho Omata,Masao Saitoh,Keiji Miyazawa

Frontiers in cell and developmental biology 9:657219 PubMed34307347

2021

Transcriptomic Analysis Identified ARHGAP Family as a Novel Biomarker Associated With Tumor-Promoting Immune Infiltration and Nanomechanical Characteristics in Bladder Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Yang,Siqi Wu,Zezhong Mou,Quan Zhou,Zheyu Zhang,Yiling Chen,Yuxi Ou,Xinan Chen,Xiyu Dai,Chenyang Xu,Na Liu,Haowen Jiang

Carcinogenesis 41:711-721 PubMed31430374

2019

ARHGAP24 inhibits cell proliferation and cell cycle progression and induces apoptosis of lung cancer via a STAT6-WWP2-p27 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Saie Shen,Haibo Xiao,Fangbao Ding,Mingsong Wang,Guoqing Li,Fengqing Hu

Medical science monitor : international medical jo 25:21-31 PubMed30599132

2019

Rho GTPase Activating Protein 24 (ARHGAP24) Silencing Promotes Lung Cancer Cell Migration and Invasion by Activating β-Catenin Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Saie Shen,Mingsong Wang,Fangbao Ding,Haibo Xiao,Guoqing Li,Fengqing Hu

Medical science monitor : international medical jo 24:8669-8677 PubMed30499465

2018

Rho GTPase Activating Protein 24 (ARHGAP24) Regulates the Anti-Cancer Activity of Sorafenib Against Breast Cancer MDA-MB-231 Cells via the Signal Transducer and Activator of Transcription 3 (STAT3) Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xianping Dai,Feng Geng,Jiale Dai,Mengshun Li,Ming Liu

Oncology letters 16:3517-3524 PubMed30127956

2018

ARHGAP24 regulates cell ability and apoptosis of colorectal cancer cells via the regulation of P53.

Applications

Unspecified application

Species

Unspecified reactive species

Suiliang Zhang,Liang Sui,Juhua Zhuang,Saifei He,Yanan Song,Ying Ye,Wei Xia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FILGAP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FILGAP antibody (AB203874)

Western blot - Anti-FILGAP antibody (AB203874)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise