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AB224371

Anti-FILGAP antibody

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(1 Publication)

Rabbit Polyclonal FILGAP antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGAP24 aa 450-650.

View Alternative Names

FILGAP, ARHGAP24, Rho GTPase-activating protein 24, Filamin-A-associated RhoGAP, RAC1- and CDC42-specific GTPase-activating protein of 72 kDa, Rho-type GTPase-activating protein 24, RhoGAP of 73 kDa, Sarcoma antigen NY-SAR-88, p73RhoGAP, FilGAP, RC-GAP72

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FILGAP antibody (AB224371)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FILGAP antibody (AB224371)

Paraffin-embedded human kidney tissue stained for FILGAP using ab224371 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ARHGAP24 aa 450-650. The exact immunogen used to generate this antibody is proprietary information.

Q8N264

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FILGAP also known as ARHGAP24 is an important regulator in the Rho family of GTPases. This protein with a mass of approximately 67 kDa serves as a GTPase-activating protein (GAP) specific for Rac1. Through its action FILGAP inhibits Rac1-mediated signals which control actin dynamics. Expression of FILGAP occurs in various tissue types with significant levels in cells involved in cytoskeletal arrangement and cell movement such as fibroblasts and endothelial cells.
Biological function summary

FILGAP influences the rearrangement of actin cytoskeleton by inactivating Rac1 which is an essential factor for lamellipodia formation and cell migration. FILGAP interacts physically with the protein Filamin A suggesting its involvement in forming a protein complex that modulates actin cytoskeleton assembly. This interaction positions FILGAP at intricate cellular junctions where mechanical stimuli affect cell structure and signaling.

Pathways

The function of FILGAP fits largely within the Rho GTPase signaling pathway and the regulation of adherens junctions. It has interactions with other proteins including but not limited to Rac1 and Cdc42 linking it to pathways controlling cell migration and adhesion. Through its regulation of Rac1 FILGAP coordinates with pathways that affect cellular morphogenesis and mechanotransduction.

Aberrant expression or function of FILGAP links to cancer progression where its regulatory role impacts cell migration and invasion capabilities. Additionally disruptions in Rho GTPase pathways involving FILGAP contribute to cardiovascular disorders such as atherosclerosis due to its role in endothelial cell movement. In both contexts its interaction with Filamin A becomes significant influencing disease mechanisms tied to cytoskeletal abnormalities and cell motility.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis.
See full target information ARHGAP24
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:7946-7960 PubMed38713160

2024

Trigeminal nerve electrical stimulation attenuates early traumatic brain injury through the TLR4/NF-κB/NLRP3 signaling pathway mediated by orexin-A/OX1R system.

Applications

Unspecified application

Species

Unspecified reactive species

Junwei Kang,Yifan Zhou,Qi Xiong,Xiaoyang Dong
View all publications
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Product promise

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