Rabbit Polyclonal FILGAP antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGAP24 aa 450-650.
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Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ARHGAP24 aa 450-650. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N264
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis.
Alternative names
Rho GTPase-activating protein 24, Filamin-A-associated RhoGAP, RAC1- and CDC42-specific GTPase-activating protein of 72 kDa, Rho-type GTPase-activating protein 24, RhoGAP of 73 kDa, Sarcoma antigen NY-SAR-88, p73RhoGAP, FilGAP, RC-GAP72, FILGAP, ARHGAP24
Recommended products
Rabbit Polyclonal FILGAP antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARHGAP24 aa 450-650.
Alternative names
Rho GTPase-activating protein 24, Filamin-A-associated RhoGAP, RAC1- and CDC42-specific GTPase-activating protein of 72 kDa, Rho-type GTPase-activating protein 24, RhoGAP of 73 kDa, Sarcoma antigen NY-SAR-88, p73RhoGAP, FilGAP, RC-GAP72, FILGAP, ARHGAP24
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ARHGAP24 aa 450-650. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N264
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FILGAP also known as ARHGAP24 is an important regulator in the Rho family of GTPases. This protein with a mass of approximately 67 kDa serves as a GTPase-activating protein (GAP) specific for Rac1. Through its action FILGAP inhibits Rac1-mediated signals which control actin dynamics. Expression of FILGAP occurs in various tissue types with significant levels in cells involved in cytoskeletal arrangement and cell movement such as fibroblasts and endothelial cells.
- Biological function summary
FILGAP influences the rearrangement of actin cytoskeleton by inactivating Rac1 which is an essential factor for lamellipodia formation and cell migration. FILGAP interacts physically with the protein Filamin A suggesting its involvement in forming a protein complex that modulates actin cytoskeleton assembly. This interaction positions FILGAP at intricate cellular junctions where mechanical stimuli affect cell structure and signaling.
- Pathways
The function of FILGAP fits largely within the Rho GTPase signaling pathway and the regulation of adherens junctions. It has interactions with other proteins including but not limited to Rac1 and Cdc42 linking it to pathways controlling cell migration and adhesion. Through its regulation of Rac1 FILGAP coordinates with pathways that affect cellular morphogenesis and mechanotransduction.
- Associated diseases and disorders
Aberrant expression or function of FILGAP links to cancer progression where its regulatory role impacts cell migration and invasion capabilities. Additionally disruptions in Rho GTPase pathways involving FILGAP contribute to cardiovascular disorders such as atherosclerosis due to its role in endothelial cell movement. In both contexts its interaction with Filamin A becomes significant influencing disease mechanisms tied to cytoskeletal abnormalities and cell motility.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FILGAP antibody (ab224371)
Paraffin-embedded human kidney tissue stained for FILGAP using ab224371 at 1/50 dilution in immunohistochemical analysis.
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