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AB224539

Anti-FIP1L1 antibody

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(1 Publication)

Rabbit Polyclonal FIP1L1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FIP1L1 aa 250-400.

View Alternative Names

FIP1, RHE, FIP1L1, Pre-mRNA 3'-end-processing factor FIP1, hFip1, FIP1-like 1 protein, Factor interacting with PAP, Rearranged in hypereosinophilia

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-FIP1L1 antibody (AB224539)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FIP1L1 antibody (AB224539)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for FIP1L1 (green) using ab224539 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIP1L1 antibody (AB224539)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIP1L1 antibody (AB224539)

Paraffin-embedded human kidney tissue stained for FIP1L1 using ab224539 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIP1L1 antibody (AB224539)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIP1L1 antibody (AB224539)

Paraffin-embedded human cerebellum tissue stained for FIP1L1 using ab224539 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIP1L1 antibody (AB224539)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIP1L1 antibody (AB224539)

Paraffin-embedded human testis tissue stained for FIP1L1 using ab224539 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-FIP1L1 antibody (AB224539)
  • WB

Unknown

Western blot - Anti-FIP1L1 antibody (AB224539)

All lanes:

Western blot - Anti-FIP1L1 antibody (ab224539) at 0.4 µg/mL

All lanes:

Human cell line MOLT4

Predicted band size: 67 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human FIP1L1 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

Q6UN15

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein FIP1L1 also known as Factor Interacting with PAPOLA and CPSF1 is a component of the mRNA processing machinery. It plays a vital role in polyadenylation an important step in mRNA processing. The protein is approximately 68 kDa in mass and is expressed in various tissues with significant levels in hematopoietic cells. This expression pattern suggests that FIP1L1 participates in processes requiring active mRNA synthesis and processing.
Biological function summary

The actions of FIP1L1 modify the mRNA polyadenylation process essential for mRNA stability and export from the nucleus. FIP1L1 acts as part of the Cleavage and Polyadenylation Specificity Factor (CPSF) complex. This complex determines the site of polyadenylation in pre-mRNA integrating signals essential for proper mRNA maturation. FIP1L1's function in this complex showcases its role in gene expression regulation and cellular messaging.

Pathways

FIP1L1 integrates into transcriptional and post-transcriptional pathways affecting mRNA processing. It interacts notably with the polyadenylation signal recognition pathway contributing to the control of RNA processing and the stability of mRNA transcripts. FIP1L1's interaction with proteins such as CPSF complex components ensures effective communications and transitions between transcriptional machinery and mRNA processing mechanisms.

FIP1L1 links especially to eosinophilic disorders including chronic eosinophilic leukemia. A small deletion in chromosome 4q12 can produce a fusion gene between FIP1L1 and PDGFRA leading to the constitutive activation of this receptor tyrosine kinase; this fusion is significant in the pathogenesis of the disease. Understanding FIP1L1’s role in this fusion helps in delineating therapeutic targets for related hematological diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. FIP1L1 contributes to poly(A) site recognition and stimulates poly(A) addition. Binds to U-rich RNA sequence elements surrounding the poly(A) site. May act to tether poly(A) polymerase to the CPSF complex.
See full target information FIP1L1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pediatrics 11:1160107 PubMed37215601

2023

Case report: Muscle involvement in a Chinese patient with -related disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Cui-Jie Wei,Yi-Dan Liu,Yan-Ling Yang,Yuan Wu,Jie-Yu Liu,Xing-Zhi Chang,Ying Hua,Yu-He Liu,Hui Xiong
View all publications
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