AB176816

Anti-FIP200 antibody

Name: Anti-FIP200 antibody (ab176816) | Abcam
Brand: Abcam Limited
SKU: AB176816
Price: 545 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal FIP200 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human RB1CC1 aa 1500 to C-terminus.

View Alternative Names

KIAA0203, RBICC, RB1CC1, RB1-inducible coiled-coil protein 1, FAK family kinase-interacting protein of 200 kDa, FIP200

3 Images

Supplier Data

Western blot - Anti-FIP200 antibody (AB176816)

All lanes:

Western blot - Anti-FIP200 antibody (ab176816) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysates at 50 µg

Lane 2:

HeLa whole cell lysates at 15 µg

Lane 3:

HeLa whole cell lysates at 5 µg

Lane 4:

293T whole cell lysates at 50 µg

Predicted band size: 183 kDa

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Exposure time: 30s

Supplier Data

Western blot - Anti-FIP200 antibody (AB176816)

All lanes:

Western blot - Anti-FIP200 antibody (ab176816) at 0.2 µg/mL

Lane 1:

TCMK1 whole cell lysates at 50 µg

Lane 2:

TCMK1 whole cell lysates at 15 µg

Predicted band size: 183 kDa

true

Exposure time: 3min

Supplier Data

Immunoprecipitation - Anti-FIP200 antibody (AB176816)

ab176816 at 0.1 μg/ml detecting FIP200 in HeLa whole cell lysate by WB following IP.

Lane 1 : ab176816 at 3μg/mg of lysate
Lane 2 : Control IgG.

In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-FIP200 antibody (ab176816)

Predicted band size: 183 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human RB1CC1 aa 1500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8TDY2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab176816 was affinity purified using an epitope specific to FIP200 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FIP200 also known as RB1CC1 is a large protein with a mass of approximately 200 kDa. It functions in several cellular processes acting as a scaffold protein that assists in the assembly of different protein complexes. FIP200 is widely expressed in human tissues including the brain heart and skeletal muscle. This suggests it has important roles in diverse biological functions within the body.

Biological function summary

FIP200 is an important component of the ULK1 complex which is a critical player in the regulation of autophagy. Autophagy is a cellular degradation pathway that removes damaged organelles and proteins helping maintain cellular homeostasis. FIP200 interacts with other proteins such as ULK1 and ATG13 to facilitate the initiation of autophagy. Its role in this complex ensures proper cellular response to nutrient deprivation and stress conditions.

Pathways

The mechanistic functions of FIP200 integrate into the autophagy and mTOR signaling pathways. In the mTOR pathway FIP200 acts upstream interacting with proteins like mTOR kinase to coordinate cell growth and autophagy. This interaction contributes to maintaining cellular energy levels and adaptions to metabolic stress. FIP200's regulatory activities within these pathways impact various cellular functions aligning cell metabolism and survival mechanisms.

FIP200's dysfunction has connections to cancer and neurodegenerative diseases. In cancer alterations in FIP200 expression can affect cell proliferation and tumorigenesis possibly through associations with proteins like p53. In neurodegenerative diseases improper autophagy due to malfunctioning FIP200 can lead to the accumulation of damaged proteins and cellular stress forging a link with disorders such as Alzheimer's. Understanding FIP200’s role in these conditions aids the exploration of therapeutic targets for disease treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Involved in autophagy (PubMed : 21775823). Regulates early events but also late events of autophagosome formation through direct interaction with Atg16L1 (PubMed : 23392225). Required for the formation of the autophagosome-like double-membrane structure that surrounds the Salmonella-containing vacuole (SCV) during S.typhimurium infection and subsequent xenophagy (By similarity). Involved in repair of DNA damage caused by ionizing radiation, which subsequently improves cell survival by decreasing apoptosis (By similarity). Inhibits PTK2/FAK1 and PTK2B/PYK2 kinase activity, affecting their downstream signaling pathways (PubMed : 10769033, PubMed : 12221124). Plays a role as a modulator of TGF-beta-signaling by restricting substrate specificity of RNF111 (By similarity). Functions as a DNA-binding transcription factor (PubMed : 12095676). Is a potent regulator of the RB1 pathway through induction of RB1 expression (PubMed : 14533007). Plays a crucial role in muscular differentiation (PubMed : 12163359). Plays an indispensable role in fetal hematopoiesis and in the regulation of neuronal homeostasis (By similarity).

See full target information RB1CC1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 36:2302-2312 PubMed34459097

2021

Silencing of miR-497-5p inhibits cell apoptosis and promotes autophagy in Parkinson's disease by upregulation of FGF2.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie Zhu,Hui Zhang,Jun Gao,Yun Xu

EMBO reports 20:e47734 PubMed31448519

2019

Targeting of early endosomes by autophagy facilitates EGFR recycling and signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Jane Fraser,Joanne Simpson,Rosa Fontana,Chieko Kishi-Itakura,Nicholas T Ktistakis,Noor Gammoh

FASEB journal : official publication of the Federa 33:8055-8068 PubMed30964699

2019

Down-regulation of exosomal microRNA-224-3p derived from bone marrow-derived mesenchymal stem cells potentiates angiogenesis in traumatic osteonecrosis of the femoral head.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Jia Xu,Wen Liao,Xiang-Zhong Liu,Jing Hu,Wen-Zhong Zou,Yu Ning,Yi Yang,Zhang-Hua Li

The EMBO journal 38: PubMed30936093

2019

Intrinsic lipid binding activity of ATG16L1 supports efficient membrane anchoring and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Leo J Dudley,Ainara G Cabodevilla,Agata N Makar,Martin Sztacho,Tim Michelberger,Joseph A Marsh,Douglas R Houston,Sascha Martens,Xuejun Jiang,Noor Gammoh

Autophagy 15:1495-1505 PubMed30821607

2019

Down-regulated TMED10 in Alzheimer disease induces autophagy via ATG4B activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Hyun Shin,So Jung Park,Doo Sin Jo,Na Yeon Park,Joon Bum Kim,Ji-Eun Bae,Yoon Kyung Jo,Jung Jin Hwang,Jin-A Lee,Dong-Gyu Jo,Jin Cheon Kim,Yong Keun Jung,Jae-Young Koh,Dong-Hyung Cho

Scientific reports 6:33229 PubMed27615604

2016

miR-224-3p inhibits autophagy in cervical cancer cells by targeting FIP200.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Fang,Shan Shu,Li Yongmei,Zhu Endong,Yin Lirong,Sun Bei

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-FIP200 antibody

Western blot - Anti-FIP200 antibody (AB176816)

Western blot - Anti-FIP200 antibody (AB176816)

Immunoprecipitation - Anti-FIP200 antibody (AB176816)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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