AB227726

Anti-FIP200 antibody

Name: Anti-FIP200 antibody (ab227726) | Abcam
Brand: Abcam Limited
SKU: AB227726
Price: 525 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(3 Publications)

Rabbit Polyclonal FIP200 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RB1CC1.

View Alternative Names

KIAA0203, RBICC, RB1CC1, RB1-inducible coiled-coil protein 1, FAK family kinase-interacting protein of 200 kDa, FIP200

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-FIP200 antibody (AB227726)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FIP200 antibody (AB227726)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for FIP200 (green) using ab227726 at 1/200 dilution in ICC/IF.

Blue : Hoechst 33342 staining.

Supplier Data

Western blot - Anti-FIP200 antibody (AB227726)

5 % SDS-PAGE gel.

All lanes:

Western blot - Anti-FIP200 antibody (ab227726) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 183 kDa

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Supplier Data

Western blot - Anti-FIP200 antibody (AB227726)

5 % SDS-PAGE gel.

All lanes:

Western blot - Anti-FIP200 antibody (ab227726) at 1/5000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

FIP200-transfected HEK-293T whole cell lysate at 30 µg

Predicted band size: 183 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human RB1CC1. The exact immunogen used to generate this antibody is proprietary information.

Q8TDY2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FIP200 also known as RB1CC1 is a large protein with a mass of approximately 200 kDa. It functions in several cellular processes acting as a scaffold protein that assists in the assembly of different protein complexes. FIP200 is widely expressed in human tissues including the brain heart and skeletal muscle. This suggests it has important roles in diverse biological functions within the body.

Biological function summary

FIP200 is an important component of the ULK1 complex which is a critical player in the regulation of autophagy. Autophagy is a cellular degradation pathway that removes damaged organelles and proteins helping maintain cellular homeostasis. FIP200 interacts with other proteins such as ULK1 and ATG13 to facilitate the initiation of autophagy. Its role in this complex ensures proper cellular response to nutrient deprivation and stress conditions.

Pathways

The mechanistic functions of FIP200 integrate into the autophagy and mTOR signaling pathways. In the mTOR pathway FIP200 acts upstream interacting with proteins like mTOR kinase to coordinate cell growth and autophagy. This interaction contributes to maintaining cellular energy levels and adaptions to metabolic stress. FIP200's regulatory activities within these pathways impact various cellular functions aligning cell metabolism and survival mechanisms.

FIP200's dysfunction has connections to cancer and neurodegenerative diseases. In cancer alterations in FIP200 expression can affect cell proliferation and tumorigenesis possibly through associations with proteins like p53. In neurodegenerative diseases improper autophagy due to malfunctioning FIP200 can lead to the accumulation of damaged proteins and cellular stress forging a link with disorders such as Alzheimer's. Understanding FIP200’s role in these conditions aids the exploration of therapeutic targets for disease treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in autophagy (PubMed : 21775823). Regulates early events but also late events of autophagosome formation through direct interaction with Atg16L1 (PubMed : 23392225). Required for the formation of the autophagosome-like double-membrane structure that surrounds the Salmonella-containing vacuole (SCV) during S.typhimurium infection and subsequent xenophagy (By similarity). Involved in repair of DNA damage caused by ionizing radiation, which subsequently improves cell survival by decreasing apoptosis (By similarity). Inhibits PTK2/FAK1 and PTK2B/PYK2 kinase activity, affecting their downstream signaling pathways (PubMed : 10769033, PubMed : 12221124). Plays a role as a modulator of TGF-beta-signaling by restricting substrate specificity of RNF111 (By similarity). Functions as a DNA-binding transcription factor (PubMed : 12095676). Is a potent regulator of the RB1 pathway through induction of RB1 expression (PubMed : 14533007). Plays a crucial role in muscular differentiation (PubMed : 12163359). Plays an indispensable role in fetal hematopoiesis and in the regulation of neuronal homeostasis (By similarity).

See full target information RB1CC1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Viruses 17: PubMed40431628

2025

The COPII Transport Complex Participates in HPV16 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia M Day,Cynthia D Thompson,Andrea S Weisberg,John T Schiller

Cell death & disease 12:188 PubMed33589600

2021

SChLAP1 promotes prostate cancer development through interacting with EZH2 to mediate promoter methylation modification of multiple miRNAs of chromosome 5 with a DNMT3a-feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Huang,Yuxin Tang

Science advances 5:eaay4624 PubMed31723608

2019

Integrated proteogenetic analysis reveals the landscape of a mitochondrial-autophagosome synapse during PARK2-dependent mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Mi Heo,Nathan J Harper,Joao A Paulo,Mamie Li,Qikai Xu,Margaret Coughlin,Stephen J Elledge,J Wade Harper

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FIP200 antibody

Immunocytochemistry/ Immunofluorescence - Anti-FIP200 antibody (AB227726)

Western blot - Anti-FIP200 antibody (AB227726)

Western blot - Anti-FIP200 antibody (AB227726)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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