Rabbit Recombinant Monoclonal FIP200 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621), expandable thumbnail](https://content.abcam.com/products/images/fip200-antibody-epr26076-87-bsa-and-azide-free-ab313621--western-blot-img418382.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621)")
![Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621), expandable thumbnail](https://content.abcam.com/products/images/fip200-antibody-epr26076-87-bsa-and-azide-free-ab313621--western-blot-img418386.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621)")
![Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621), expandable thumbnail](https://content.abcam.com/products/images/fip200-antibody-epr26076-87-bsa-and-azide-free-ab313621--western-blot-img418385.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621)")
![Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621), expandable thumbnail](https://content.abcam.com/products/images/fip200-antibody-epr26076-87-bsa-and-azide-free-ab313621--western-blot-img418384.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621)")
![Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621), expandable thumbnail](https://content.abcam.com/products/images/fip200-antibody-epr26076-87-bsa-and-azide-free-ab313621--western-blot-img418383.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | IHC-P | |
|---|---|---|---|
| Human | Tested | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Involved in autophagy (PubMed:21775823). Regulates early events but also late events of autophagosome formation through direct interaction with Atg16L1 (PubMed:23392225). Required for the formation of the autophagosome-like double-membrane structure that surrounds the Salmonella-containing vacuole (SCV) during S.typhimurium infection and subsequent xenophagy (By similarity). Involved in repair of DNA damage caused by ionizing radiation, which subsequently improves cell survival by decreasing apoptosis (By similarity). Inhibits PTK2/FAK1 and PTK2B/PYK2 kinase activity, affecting their downstream signaling pathways (PubMed:10769033, PubMed:12221124). Plays a role as a modulator of TGF-beta-signaling by restricting substrate specificity of RNF111 (By similarity). Functions as a DNA-binding transcription factor (PubMed:12095676). Is a potent regulator of the RB1 pathway through induction of RB1 expression (PubMed:14533007). Plays a crucial role in muscular differentiation (PubMed:12163359). Plays an indispensable role in fetal hematopoiesis and in the regulation of neuronal homeostasis (By similarity).
Alternative names
KIAA0203, RBICC, RB1CC1, RB1-inducible coiled-coil protein 1, FAK family kinase-interacting protein of 200 kDa, FIP200
Recommended products
Rabbit Recombinant Monoclonal FIP200 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26076-87
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FIP200 also known as RB1CC1 is a large protein with a mass of approximately 200 kDa. It functions in several cellular processes acting as a scaffold protein that assists in the assembly of different protein complexes. FIP200 is widely expressed in human tissues including the brain heart and skeletal muscle. This suggests it has important roles in diverse biological functions within the body.
- Biological function summary
FIP200 is an important component of the ULK1 complex which is a critical player in the regulation of autophagy. Autophagy is a cellular degradation pathway that removes damaged organelles and proteins helping maintain cellular homeostasis. FIP200 interacts with other proteins such as ULK1 and ATG13 to facilitate the initiation of autophagy. Its role in this complex ensures proper cellular response to nutrient deprivation and stress conditions.
- Pathways
The mechanistic functions of FIP200 integrate into the autophagy and mTOR signaling pathways. In the mTOR pathway FIP200 acts upstream interacting with proteins like mTOR kinase to coordinate cell growth and autophagy. This interaction contributes to maintaining cellular energy levels and adaptions to metabolic stress. FIP200's regulatory activities within these pathways impact various cellular functions aligning cell metabolism and survival mechanisms.
- Associated diseases and disorders
FIP200's dysfunction has connections to cancer and neurodegenerative diseases. In cancer alterations in FIP200 expression can affect cell proliferation and tumorigenesis possibly through associations with proteins like p53. In neurodegenerative diseases improper autophagy due to malfunctioning FIP200 can lead to the accumulation of damaged proteins and cellular stress forging a link with disorders such as Alzheimer's. Understanding FIP200’s role in these conditions aids the exploration of therapeutic targets for disease treatment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (ab313621), expandable thumbnail](https://content.abcam.com/products/images/fip200-antibody-epr26076-87-bsa-and-azide-free-ab313621--western-blot-img418382.jpg "Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (ab313621)")
Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (ab313621)
FIP200 Western blot staining using rabbit Anti-FIP200 antibody
This data was developed using Anti-FIP200 antibody [EPR26076-87] ab313620, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) loading control staining at 1/10000 dilution.
Exposure time: 180 secondsAll lanes: Western blot - Anti-FIP200 antibody [EPR26076-87] (Anti-FIP200 antibody [EPR26076-87] ab313620) at 1/1000 dilution
Lane 1: Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg
Lane 2: HeLa treated with 2uM bafilomycin A1 for 18h whole cell lysate at 50 µg
Lane 3: Untreated MEF (mouse embryo fibroblast) whole cell lysate at 50 µg
Lane 4: MEF treated with 2uM bafilomycin A1 for 18h whole cell lysate at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 200 kDa
Exposure time: 180s
![Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (ab313621), expandable thumbnail](https://content.abcam.com/products/images/fip200-antibody-epr26076-87-bsa-and-azide-free-ab313621--western-blot-img418386.jpg "Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (ab313621)")
Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (ab313621)
FIP200 Western blot staining using rabbit Anti-FIP200 antibody
This data was developed using Anti-FIP200 antibody [EPR26076-87] ab313620, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
The identity of the lower MW bands at approximately 100 kDa are unknown.
In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) loading control staining at 1/10000 dilution.
All lanes: Western blot - Anti-FIP200 antibody [EPR26076-87] (Anti-FIP200 antibody [EPR26076-87] ab313620) at 1/1000 dilution
Lane 1: Untreated A-172 (human brain glioblastoma ) whole cell lysate at 50 µg
Lane 2: A-172 treated with 50uM chloroquine for 8h whole cell lysate at 50 µg
Lane 3: Untreated C2C12 (mouse myoblast) whole cell lysate at 50 µg
Lane 4: C2C12 treated with 50uM chloroquine for 24h whole cell lysate at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 200 kDa
Exposure time: 180s
![Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (ab313621), expandable thumbnail](https://content.abcam.com/products/images/fip200-antibody-epr26076-87-bsa-and-azide-free-ab313621--western-blot-img418385.jpg "Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (ab313621)")
Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (ab313621)
This data was developed using Anti-FIP200 antibody [EPR26076-87] ab313620, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-FIP200 antibody [EPR26076-87] (Anti-FIP200 antibody [EPR26076-87] ab313620) at 1/1000 dilution
All lanes: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 200 kDa
Exposure time: 180s
![Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (ab313621), expandable thumbnail](https://content.abcam.com/products/images/fip200-antibody-epr26076-87-bsa-and-azide-free-ab313621--western-blot-img418384.jpg "Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (ab313621)")
Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (ab313621)
This data was developed using Anti-FIP200 antibody [EPR26076-87] ab313620, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 37 secondsAll lanes: Western blot - Anti-FIP200 antibody [EPR26076-87] (Anti-FIP200 antibody [EPR26076-87] ab313620) at 1/1000 dilution
All lanes: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 50 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 200 kDa
Exposure time: 37s
![Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (ab313621), expandable thumbnail](https://content.abcam.com/products/images/fip200-antibody-epr26076-87-bsa-and-azide-free-ab313621--western-blot-img418383.jpg "Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (ab313621)")
Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (ab313621)
This data was developed using Anti-FIP200 antibody [EPR26076-87] ab313620, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 10 secondsAll lanes: Western blot - Anti-FIP200 antibody [EPR26076-87] (Anti-FIP200 antibody [EPR26076-87] ab313620) at 1/1000 dilution
Lane 1: Mouse testis tissue lysate at 50 µg
Lane 2: Rat testis tissue lysate at 50 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 200 kDa
Exposure time: 10s
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