Anti-Firefly Luciferase antibody

• WB

Lab

Western blot - Anti-Firefly Luciferase antibody (AB181640)

Lanes 1 - 4 : Merged signal (red and green). Green – ab181640 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab181640 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Donkey anti-Goat IgG H&L (IRDye® 800CW) preabsorbed (ab216775) and Donkey anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216778) at a 1 : 10000 dilution for 1hr at room temperature and then imaged.

All lanes:

Western blot - Anti-Firefly Luciferase antibody (ab181640) at 1/1000 dilution

Lane 1:

Non-transfected 293 cell lysate at 20 µg

Lane 2:

Firefly Luciferase transfected 293 cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

NIH3T3 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Donkey anti-Goat IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/donkey-goat-igg-h-l-irdye-800cw-preadsorbed-ab216775'>ab216775</a>) at 1/10000 dilution

Predicted band size: 61 kDa

Observed band size: 65 kDa

false

• WB

Supplier Data

Western blot - Anti-Firefly Luciferase antibody (AB181640)

All lanes:

Western blot - Anti-Firefly Luciferase antibody (ab181640) at 1/1000 dilution

All lanes:

Luciferase protein at 0.05 µg

Secondary

All lanes:

Peroxidase anti-goat secondary antibody at 1/40000 dilution

Predicted band size: 61 kDa

false