Anti-Firefly Luciferase antibody

• ICC/IF

AbReview10285****

Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (AB21176)

ab21176 staining mouse mammary carcinoma cells by ICC/IF.

Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking with a commercial blocking agent. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 25°C. An Alexa-Fluor^® 555 conjugated goat anti-rabbit antibody was used as the secondary.

The image shows firefly luciferase (red) in mouse tumour cells and cell nuclei counterstained with Hoechst (blue).

This image is courtesy of an anonymous Abreview

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (AB21176)

ab21176 at 10 μg/mL staining Luciferase in transfected HEK-293 (Human epithelial cell line from embryonic kidney) cells by ICC/IF.

The cells were fixed with methanol and acetone. An FITC conjugated anti-Rabbit IgG was used as the secondary antibody.

Left panel : Un-transfected cells.

Right panel : Transfected cells.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (AB21176)

Immunocytochemical analysis analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells overexpressing luciferase labeling Luciferase with ab21176 at a concentration of 10 μg/mL. The secondary antibody was a Goat anti-Rabbit IgG, FITC conjugate.

• ICC/IF

AbReview5843****

Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (AB21176)

ab21176 at 1/200 dilution staining engineered adult rat stromal stem cells by ICC/IF.

The cells were fixed in 2% paraformaldehyde and 0.1% Triton X-100 was used for cell permeabilization (15 minutes incubation time). The cells were incubated with the antibody overnight at 4°C. The image shows Luciferase (green-upper right panel), counterstained cell nuclei (DAPI-blue-upper left panel), overlay (lower left panel) and a phase contrast image (lower right panel).

The image was taken with a confocal laser scanning microscope equipped with an additional laser differential Interference Contrast (DIC) mode.

This image is courtesy of an Abreview submitted by Dr Mal Niladri

• WB

Supplier Data

Western blot - Anti-Firefly Luciferase antibody (AB21176)

All lanes:

Western blot - Anti-Firefly Luciferase antibody (ab21176) at 1/1000 dilution

Lane 1:

Lysates from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells overexpressing luciferase

Lane 2:

Lysates from HEK-293T cells overexpressing luciferase with Luciferase Immunizing Peptide

Secondary

All lanes:

Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate

false