Anti-Firefly Luciferase antibody is a rabbit polyclonal antibody that is used to detect Firefly Luciferase in ICC/IF, Western blot. Suitable for Firefly samples.
- Cited in over 80 publications
- Tried and trusted by researchers since 2005
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- Cells 12:2022iNOS Deletion in Alveolar Epithelium Cannot Reverse the Elastase-Induced Emphysema in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarija Gredic,Vinita Sharma,Stefan Hadzic,Cheng-Yu Wu,Oleg Pak,Baktybek Kojonazarov,Julia Duerr,Marcus A Mall,Andreas Guenther,Ralph T Schermuly,Friedrich Grimminger,Werner Seeger,Simone Kraut,Natascha Sommer,Norbert WeissmannPubMed 36611917
- Acta biochimica et biophysica Sinica 54:1507-15172022Bioluminescence imaging of mouse monocyte chemoattractant protein-1 expression in inflammatory processes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFangyang Shao,Lei Ci,Jiahao Shi,Fei Fang,Bowen Yan,Xijun Liu,Xiangyu Yao,Mengjie Zhang,Hua Yang,Zhugang Wang,Jian FeiPubMed 36239355
- Viruses 14:2022A Novel Piggyback Strategy for mRNA Delivery Exploiting Adenovirus Entry Biology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMyungeun Lee,Paul J Rice-Boucher,Logan Thrasher Collins,Ernst Wagner,Lorenzo Aulisa,Jeffrey Hughes,David T CurielPubMed 36298724
- Cancer discovery 13:85-972022Cell Competition Shapes Metastatic Latency and Relapse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKangsan Kim,Huocong Huang,Pravat Kumar Parida,Lan He,Mauricio Marquez-Palencia,Tanner C Reese,Payal Kapur,James Brugarolas,Rolf A Brekken,Srinivas MalladiPubMed 36098678
- Cell metabolism 34:90-105.e72022Metabolic diversity within breast cancer brain-tropic cells determines metastatic fitness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPravat Kumar Parida,Mauricio Marquez-Palencia,Vidhya Nair,Akash K Kaushik,Kangsan Kim,Jessica Sudderth,Eduardo Quesada-Diaz,Ambar Cajigas,Vamsidhara Vemireddy,Paula I Gonzalez-Ericsson,Melinda E Sanders,Bret C Mobley,Kenneth Huffman,Sunati Sahoo,Prasanna Alluri,Cheryl Lewis,Yan Peng,Robert M Bachoo,Carlos L Arteaga,Ariella B Hanker,Ralph J DeBerardinis,Srinivas MalladiPubMed 34986341
- Communications biology 4:13502021ER-misfolded proteins become sequestered with mitochondria and impair mitochondrial function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdrián Cortés Sanchón,Harshitha Santhosh Kumar,Matilde Mantovani,Ivan Osinnii,José María Mateos,Andres Kaech,Dimitri Shcherbakov,Rashid Akbergenov,Erik C BöttgerPubMed 34857875
- Journal for immunotherapy of cancer 9:2021Synergy between type I Δ immunotherapy and PD-L1 checkpoint inhibition triggers the regression of targeted and distal tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Chao Zhu,Hany M Elsheikha,Jian-Hua Wang,Shuai Fang,Jun-Jun He,Xing-Quan Zhu,Jia ChenPubMed 34725213
- Journal of virology 95:e00707212021Th17 T Cells and Immature Dendritic Cells Are the Preferential Initial Targets after Rectal Challenge with a Simian Immunodeficiency Virus-Based Replication-Defective Dual-Reporter Vector.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanijela Maric,Wesley A Grimm,Natalie Greco,Michael D McRaven,Angela J Fought,Ronald S Veazey,Thomas J HopePubMed 34287053
- Nature 597:132-1372021DAXX represents a new type of protein-folding enabler.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiangqian Huang,Trisha Agrawal,Guixin Zhu,Sixiang Yu,Liming Tao,JiaBei Lin,Ronen Marmorstein,James Shorter,Xiaolu YangPubMed 34408321
- Proceedings of the National Academy of Sciences of the United States of America 118:2021Hypoimmune induced pluripotent stem cell-derived cell therapeutics treat cardiovascular and pulmonary diseases in immunocompetent allogeneic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTobias Deuse,Grigol Tediashvili,Xiaomeng Hu,Alessia Gravina,Annika Tamenang,Dong Wang,Andrew Connolly,Christian Mueller,Beñat Mallavia,Mark R Looney,Malik Alawi,Lewis L Lanier,Sonja SchrepferPubMed 34244428
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Native Full Length Protein corresponding to Firefly LUCI_PHOPY. Database link P08659
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Firefly | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Firefly | Dilution info 1/1000 - 1/2000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Firefly | Dilution info 10 µg/mL | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
Target data
Function
Produces green light with a wavelength of 562 nm.
Alternative names
Luciferin 4-monooxygenase, Luciferase
Recommended products
Anti-Firefly Luciferase antibody is a rabbit polyclonal antibody that is used to detect Firefly Luciferase in ICC/IF, Western blot. Suitable for Firefly samples.
- Cited in over 80 publications
- Tried and trusted by researchers since 2005
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Native Full Length Protein corresponding to Firefly LUCI_PHOPY. Database link P08659
- Purity
- IgG fraction
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Firefly Luciferase antibody (ab21176) is a rabbit polyclonal antibody and is validated for use in ICC/IF, WB in human, mouse samples.
Anti-Firefly Luciferase antibody (ab21176) has been cited over 80 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Firefly Luciferase antibody (ab21176) has high sensitivity and specificity.
Anti-Firefly Luciferase antibody (ab21176) has 18 independent reviews from customers.
Anti-Firefly Luciferase antibody (ab21176) specifically detects Firefly Luciferase (UniProt ID: P13129; Molecular weight: 60kDa) and is sold in 100 µL selling sizes.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Firefly luciferase also known as luc assay or luciferase protein is an enzyme that catalyzes the oxidation of luciferin resulting in bioluminescence. This protein has a molecular mass of approximately 61 kDa and is responsible for the characteristic glow of fireflies. The luciferase expression occurs mainly in the light-producing organs of fireflies such as in the insect Photinus pyralis where it plays an important role in light emission.
- Biological function summary
Firefly luciferase converts chemical energy into light making it valuable for various biological applications as a reporter gene. It participates in the formation of a complex with its substrates luciferin and ATP resulting in the production of a photon. This enzyme is widely used in molecular and cellular biology experiments to monitor gene expression and study protein-protein interactions due to its luminescent properties.
- Pathways
Firefly luciferase functions in the bioluminescent pathway involving the conversion of luciferin to oxyluciferin and light emission. It interfaces with ATP a universal energy carrier which highlights its integration into the cellular energy systems. Firefly luciferase is similar to other oxidative enzymes though it is not directly involved with metabolic pathways like the TCA cycle or glycolysis.
- Associated diseases and disorders
Researchers often utilize firefly luciferase as a tool in tumor studies or infectious disease models. Its luminescence aids in imaging and tracking biological processes in real-time. While firefly luciferase itself is not directly associated with specific diseases the use of firefly agents allows researchers to study disease outcomes assess therapeutic efficacy and understand gene expression profiles related to cancer and viral infections often in connection with proteins such as p53 in cancer research.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
This image is courtesy of an anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (ab21176)
ab21176 staining mouse mammary carcinoma cells by ICC/IF.
Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking with a commercial blocking agent. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 25°C. An Alexa-Fluor® 555 conjugated goat anti-rabbit antibody was used as the secondary.
The image shows firefly luciferase (red) in mouse tumour cells and cell nuclei counterstained with Hoechst (blue).
Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (ab21176)
ab21176 at 10 μg/mL staining Luciferase in transfected HEK-293 (Human epithelial cell line from embryonic kidney) cells by ICC/IF.
The cells were fixed with methanol and acetone. An FITC conjugated anti-Rabbit IgG was used as the secondary antibody.
Left panel: Un-transfected cells.
Right panel: Transfected cells.
This image is courtesy of a customer review submitted by Dr Mal NiladriImmunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (ab21176)
ab21176 at 1/200 dilution staining engineered adult rat stromal stem cells by ICC/IF.
The cells were fixed in 2% paraformaldehyde and 0.1% Triton X-100 was used for cell permeabilization (15 minutes incubation time). The cells were incubated with the antibody overnight at 4°C. The image shows Luciferase (green-upper right panel), counterstained cell nuclei (DAPI-blue-upper left panel), overlay (lower left panel) and a phase contrast image (lower right panel).
The image was taken with a confocal laser scanning microscope equipped with an additional laser differential Interference Contrast (DIC) mode.
Western blot - Anti-Firefly Luciferase antibody (ab21176)
All lanes: Western blot - Anti-Firefly Luciferase antibody (ab21176) at 1/1000 dilution
Lane 1: Lysates from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells overexpressing luciferase
Lane 2: Lysates from HEK-293T cells overexpressing luciferase with Luciferase Immunizing Peptide
SecondaryAll lanes: Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate
Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (ab21176)
Immunocytochemical analysis analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells overexpressing luciferase labeling Luciferase with ab21176 at a concentration of 10 μg/mL. The secondary antibody was a Goat anti-Rabbit IgG, FITC conjugate.
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