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Anti-Firefly Luciferase antibody [EPR17790] is a rabbit recombinant monoclonal antibody that is used to detect Firefly Luciferase in Flow cytometry, ICC/IF, Western blot. Suitable for Firefly samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Cited in over 10 publications


Images

Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17790] (AB185924), expandable thumbnail
  • Flow Cytometry - Anti-Firefly Luciferase antibody [EPR17790] (AB185924), expandable thumbnail
  • Western blot - Anti-Firefly Luciferase antibody [EPR17790] (AB185924), expandable thumbnail
  • Western blot - Anti-Firefly Luciferase antibody [EPR17790] (AB185924), expandable thumbnail
  • Western blot - Anti-Firefly Luciferase antibody [EPR17790] (AB185924), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Human
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Firefly
Predicted
Not recommended
Predicted
Transfected cell line - Firefly
Tested
Not recommended
Tested
Transfected cell lysate - Firefly
Not recommended
Tested
Not recommended

Tested
Tested

Species
Transfected cell line - Firefly
Dilution info
-
Notes

-

Predicted
Predicted

Species
Firefly
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Transfected cell lysate - Firefly, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell lysate - Firefly
Dilution info
1/20000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Firefly, Transfected cell line - Firefly
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Firefly
Dilution info
1/500
Notes

-

Predicted
Predicted

Species
Firefly
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Transfected cell lysate - Firefly, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

Produces green light with a wavelength of 562 nm.

Alternative names

Luciferin 4-monooxygenase, Luciferase

Recommended products

Anti-Firefly Luciferase antibody [EPR17790] is a rabbit recombinant monoclonal antibody that is used to detect Firefly Luciferase in Flow cytometry, ICC/IF, Western blot. Suitable for Firefly samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Cited in over 10 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17790
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Anti-Firefly Luciferase antibody [EPR17790] (ab185924) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt, ICC/IF, WB in firefly samples.

Anti-Firefly Luciferase antibody [EPR17790] (ab185924) has been cited over 18 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality manufacturing and validation processes ensure Anti-Firefly Luciferase antibody [EPR17790] (ab185924) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-Firefly Luciferase antibody [EPR17790] (ab185924) specifically detects Firefly Luciferase (UniProt ID: P08659; Molecular weight: 61kDa) and is sold in 100 uL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR17790 - Anti-Firefly Luciferase antibody [EPR17790] - BSA and Azide free ab232629.

Antibody clone EPR17790 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647 (ab21495, ab23349).

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Firefly luciferase also known as luc assay or luciferase protein is an enzyme that catalyzes the oxidation of luciferin resulting in bioluminescence. This protein has a molecular mass of approximately 61 kDa and is responsible for the characteristic glow of fireflies. The luciferase expression occurs mainly in the light-producing organs of fireflies such as in the insect Photinus pyralis where it plays an important role in light emission.

Biological function summary

Firefly luciferase converts chemical energy into light making it valuable for various biological applications as a reporter gene. It participates in the formation of a complex with its substrates luciferin and ATP resulting in the production of a photon. This enzyme is widely used in molecular and cellular biology experiments to monitor gene expression and study protein-protein interactions due to its luminescent properties.

Pathways

Firefly luciferase functions in the bioluminescent pathway involving the conversion of luciferin to oxyluciferin and light emission. It interfaces with ATP a universal energy carrier which highlights its integration into the cellular energy systems. Firefly luciferase is similar to other oxidative enzymes though it is not directly involved with metabolic pathways like the TCA cycle or glycolysis.

Associated diseases and disorders

Researchers often utilize firefly luciferase as a tool in tumor studies or infectious disease models. Its luminescence aids in imaging and tracking biological processes in real-time. While firefly luciferase itself is not directly associated with specific diseases the use of firefly agents allows researchers to study disease outcomes assess therapeutic efficacy and understand gene expression profiles related to cancer and viral infections often in connection with proteins such as p53 in cancer research.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17790] (ab185924), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Firefly Luciferase, labeling Firefly Luciferase with ab185924 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK293T cells transfected with Firefly Luciferase is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    -ve control 1: ab185924 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Flow Cytometry - Anti-Firefly Luciferase antibody [EPR17790] (ab185924), expandable thumbnail

    Flow Cytometry - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)

    Flow Cytometry analysis of 293T (Human epithelial cell line from embryonic kidney) cells transfected with GFP tagged Firefly Luciferase labeled with purified ab185924 at 1/70 dilution (10μg/ml, Right panel). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 647)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Left panel) was used as the isotype control.

  • Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924), expandable thumbnail

    Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924) at 1/20000 dilution

    Lane 1: Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate at 10 µg

    Lane 2: Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 61 kDa

    Observed band size: 61 kDa

    Exposure time: 3min

  • Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924), expandable thumbnail

    Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924) at 1/1000 dilution

    Lane 1: Firefly Luciferase transfected HEK293 (human embryonic kidney) whole cell lysate at 10 µg

    Lane 2: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 3: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 4: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

    Lane 5: SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 20 µg

    Lane 6: Human fetal brain lysate at 10 µg

    Lane 7: Human fetal heart lysate at 10 µg

    Lane 8: Human fetal kidney lysate at 10 µg

    Lane 9: Human fetal spleen lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 61 kDa

    Observed band size: 61 kDa

    Exposure time: 3min

  • Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924), expandable thumbnail

    Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Firefly Luciferase antibody [EPR17790] (ab185924) at 1/1000 dilution

    Lane 1: Firefly Luciferase transfected HEK293 (Human embryonic kidney) whole cell lysate at 10 µg

    Lane 2: Mouse brain lysate at 10 µg

    Lane 3: Mouse heart lysate at 10 µg

    Lane 4: Mouse kidney lysate at 10 µg

    Lane 5: Mouse spleen lysate at 10 µg

    Lane 6: Rat brain lysate at 10 µg

    Lane 7: Rat heart lysate at 10 µg

    Lane 8: Rat kidney lysate at 10 µg

    Lane 9: Rat spleen lysate at 10 µg

    Lane 10: C6 (Rat glial tumor cells) whole cell lysate at 10 µg

    Lane 11: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 12: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

    Lane 13: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 61 kDa

    Observed band size: 61 kDa

    Exposure time: 3min

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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