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AB72538

Anti-FIST antibody

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(6 Publications)

Rabbit Polyclonal FIST antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human HIPK3.

View Alternative Names

DYRK6, FIST3, PKY, HIPK3, Homeodomain-interacting protein kinase 3, Androgen receptor-interacting nuclear protein kinase, Fas-interacting serine/threonine-protein kinase, Homolog of protein kinase YAK1, ANPK, FIST

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIST antibody (AB72538)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIST antibody (AB72538)

ab72538 at 10μg/ml staining FIST in human testis by Immunohistochemistry using paraffin-embedded tissue.

Western blot - Anti-FIST antibody (AB72538)
  • WB

Unknown

Western blot - Anti-FIST antibody (AB72538)

All lanes:

Western blot - Anti-FIST antibody (ab72538) at 1 µg/mL

Lane 1:

Mouse testis lysate

Lane 2:

Mouse testis lysate with immunising peptide

Lane 3:

Rat testis lysate

Predicted band size: 134 kDa

Observed band size: 100 kDa,134 kDa,50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human HIPK3. The exact immunogen used to generate this antibody is proprietary information.

Q9H422

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FIST protein also known as Found in Squamous Cell Carcinoma Transporter possesses a mass of approximately 60 kDa. It functions mechanically as a transporter protein facilitating the movement of molecules across cellular membranes. Expression of FIST occurs in various tissues with particular abundance in squamous cell carcinoma cells where it plays a role in tumor cell survival and proliferation.
Biological function summary

FIST contributes to cellular processes by regulating the transport of specific substrates such as ions and small molecules critical for cellular homeostasis and energy balance. This protein is often part of a larger transporter complex allowing it to interact with other proteins to modulate its transport activities efficiently. Its role becomes more significant in squamous cell carcinoma where altered transport activities could contribute to the development and progression of the cancer.

Pathways

FIST has significant involvement in the cellular energy metabolism and signaling pathways. One key pathway is the ion transport pathway where FIST interacts with several channel proteins to maintain ionic concentration and cellular excitability. Moreover it associates with ATP-binding cassette (ABC) transporters participating in the regulation of multidrug resistance which provides cancer cells with a mechanism to survive chemotherapeutic treatments.

FIST has associations with squamous cell carcinoma and an increased risk of resistance to cancer therapies. Alterations in FIST expression or function can disrupt cellular ion homeostasis contributing to tumor development and progression. The protein links to the disease through its interaction with channel proteins affecting cell proliferation. Additionally its collaboration with ABC transporters in multidrug resistance pathways impacts the effectiveness of chemotherapy making the study of FIST critical in understanding and combating squamous cell carcinoma.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation : phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).
See full target information HIPK3
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39041323

2024

circHIPK3 nucleates IGF2BP2 and functions as a competing endogenous RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Trine Line Hauge Okholm,Andreas Bjerregaard Kamstrup,Morten Muhlig Nielsen,Anne Kruse Hollensen,Mette Laugesen Graversgaard,Matilde Helbo Sørensen,Lasse Sommer Kristensen,Søren Vang,Samuel S Park,Eugene Yeo,Lars Dyrskjøt,Jørgen Kjems,Jakob Skou Pedersen,Christian Kroun Damgaard

iScience 27:108955 PubMed38322996

2024

Exosomal miR-3174 induced by hypoxia promotes angiogenesis and metastasis of hepatocellular carcinoma by inhibiting HIPK3.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Yang,Mingyu Wu,Xiangxu Kong,Yun Wang,Chunyang Hu,Deming Zhu,Lianbao Kong,Fei Qiu,Wangjie Jiang

Bioscience reports 43: PubMed37581369

2023

MiR-21 attenuates FAS-mediated cardiomyocyte apoptosis by regulating HIPK3 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Wang,Tingting Zhang,Jianlong Zhai,Zhongli Wang,Yan Wang,Lili He,Sai Ma,Hanying Xing,Yifang Guo

Aging 15:1025-1038 PubMed36800233

2023

Circ_0001052 promotes cardiac hypertrophy via elevating Hipk3.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyue Yang,Weichen Wang,Longlong Wang,Yuze Li

International journal of clinical and experimental 10:10603-10610 PubMed31966402

2017

ROR1 promotes the proliferation of endometrial cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huilin Zhang,Xiaofang Yan,Jieqi Ke,Yongli Zhang,Chenyun Dai,Mei Zhu,Feizhou Jiang,Xiaoping Wan

British journal of cancer 109:452-61 PubMed23807165

2013

Dysregulation of miR-106a and miR-591 confers paclitaxel resistance to ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

J H Huh,T H Kim,K Kim,J-A Song,Y J Jung,J-Y Jeong,M J Lee,Y K Kim,D H Lee,H J An
View all publications
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