Rabbit Polyclonal 6X His tag® antibody - conjugated to FITC. Suitable for Flow Cyt, ELISA and reacts with Tag samples. Cited in 29 publications.
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- mAbs 14:20216012022Computational design of a neutralizing antibody with picomolar binding affinity for all concerning SARS-CoV-2 variants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo-Seong Jeong,Jeong Seok Cha,Insu Hwang,Uijin Kim,Jared Adolf-Bryfogle,Brian Coventry,Hyun-Soo Cho,Kyun-Do Kim,Byung-Ha OhPubMed 35030983
- International journal of molecular sciences 23:2022Syndecan-4 Is a Key Facilitator of the SARS-CoV-2 Delta Variant's Superior Transmission.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnett Hudák,Gábor Veres,Annamária Letoha,László Szilák,Tamás LetohaPubMed 35054983
- The Journal of experimental medicine 218:2021HVEM structures and mutants reveal distinct functions of binding to LIGHT and BTLA/CD160.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeifeng Liu,Ting-Fang Chou,Sarah C Garrett-Thomson,Goo-Young Seo,Elena Fedorov,Udupi A Ramagopal,Jeffrey B Bonanno,Qingyang Wang,Kenneth Kim,Scott J Garforth,Kiyokazu Kakugawa,Hilde Cheroutre,Mitchell Kronenberg,Steven C AlmoPubMed 34709351
- Molecular oncology 16:485-5072021Recombinant nanobody against MUC1 tandem repeats inhibits growth, invasion, metastasis, and vascularization of spontaneous mouse mammary tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesParnaz Merikhian,Behrad Darvishi,Neda Jalili,Mohammad Reza Esmailinejad,Azadeh Sharif Khatibi,Shima Moradi Kalbolandi,Malihe Salehi,Marjan Mosayebzadeh,Mahdieh Shokrollahi Barough,Keivan Majidzadeh-A,Fatemeh Yadegari,Fatemeh Rahbarizadeh,Leila FarahmandPubMed 34694686
- eLife 10:2021Chitinase 3-like-1 contributes to acetaminophen-induced liver injury by promoting hepatic platelet recruitment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhao Shan,Leike Li,Constance Lynn Atkins,Meng Wang,Yankai Wen,Jongmin Jeong,Nicolas F Moreno,Dechun Feng,Xun Gui,Ningyan Zhang,Chun Geun Lee,Jack A Elias,William M Lee,Bin Gao,Fong Wilson Lam,Zhiqiang An,Cynthia JuPubMed 34110284
- Oncology reports :2020Novel EGFR‑bispecific recombinant immunotoxin based on cucurmosin shows potent anti‑tumor efficiency in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaiyun Zhang et. al.PubMed 33416157
- Cancer science 111:3184-31942020Novel cucurmosin-based immunotoxin targeting programmed cell death 1-ligand 1 with high potency against human tumor in vitro and in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaiyun Zhang et. al.PubMed 32589330
- Nature chemical biology 16:15-232019Structural basis of indisulam-mediated RBM39 recruitment to DCAF15 E3 ligase complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDirksen E Bussiere,Lili Xie,Honnappa Srinivas,Wei Shu,Ashley Burke,Celine Be,Junping Zhao,Adarsh Godbole,Dan King,Rajeshri G Karki,Viktor Hornak,Fangmin Xu,Jennifer Cobb,Nathalie Carte,Andreas O Frank,Alexandra Frommlet,Patrick Graff,Mark Knapp,Aleem Fazal,Barun Okram,Songchun Jiang,Pierre-Yves Michellys,Rohan Beckwith,Hans Voshol,Christian Wiesmann,Jonathan M Solomon,Joshiawa PaulkPubMed 31819272
- Applied microbiology and biotechnology 103:7703-77172019In vitro affinity maturation of antibody against membrane-bound GPCR molecules.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Wang,Lili An,Yun Zhao,Cheng Zhang,Shengnan Li,Chen Ye,Shuqian Jing,Haiying HangPubMed 31359103
- Artificial cells, nanomedicine, and biotechnology 47:2882-28902019Ras-AKT signaling represses the phosphorylation of histone H1.5 at threonine 10 via GSK3 to promote the progression of glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBen Sang,Jianjing Sun,Dongxu Yang,Zhen Xu,Yuzhen WeiPubMed 31307224
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- FITC
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA- Form
- Liquid
- Clonality
- Polyclonal
Reactivity data
Select an application| Flow Cyt | ELISA | |
|---|---|---|
| Tag | Tested | Expected |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Alternative names
6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag
Recommended products
Rabbit Polyclonal 6X His tag® antibody - conjugated to FITC. Suitable for Flow Cyt, ELISA and reacts with Tag samples. Cited in 29 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- FITC
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
FITC/Protein Ratio = 3.0 – 6.0.
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HIS-TAG® is a trademark of EMD Biosciences, Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.
- Biological function summary
Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.
- Pathways
The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.
- Associated diseases and disorders
The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Li et al PLoS One. 2018 Jan 22;13(1):e0191024. doi: 10.1371/journal.pone.0191024. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Flow Cytometry - FITC Anti-6X His tag® antibody (ab1206)
Muc1-Bi-1 and Muc1-Bi-2 can recognize Muc1 positive tumor cells.a). Flow cytometry analysis of Muc1-Bi-1 and Muc1-Bi-2 on Muc1-negative cell lines HePG2 and CHO and Muc1-positive cell lines, HT29. Red line indicates cells with no staining; green line indicates cells with only anti-His-FITC staining (ab1206); blue line indicates cells with positive control anti-Muc1 antibody; purple line indicates cells with Muc1-Bi-1 protein; orange line indicates cells with Muc1-Bi-2 protein.
This image is courtesy of an anonymous customer reviewFlow Cytometry - FITC Anti-6X His tag® antibody (ab1206)
ab1206 staining 6X His tag® in human endometrial carcinoma cells by Flow Cytometry.
Cells were prepared by short trypsinisation in PBS/1% FBS/0.2% sodium azide and fixed with formaldehyde. The gate included all single cells. The sample was incubated with a 6X His tag® primary antibody for 1 hour at 4°C. ab1206 was used as the secondary. On the image, the red line represents cells stained with ab1206 alone, and the blue line is the tested sample carrying a 6XHis tag®. The small peak to the right represents cells that bound the tested sample.
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