AB3554

FITC Anti-6X His tag® antibody

Name: FITC Anti-6X His tag® antibody (ab3554) | Abcam
Brand: Abcam Limited
SKU: AB3554
Rating: 5 (1 reviews)

(1 Review)

(7 Publications)

Chicken Polyclonal 6X His tag® antibody - conjugated to FITC. Suitable for ELISA, WB and reacts with Tag samples. Cited in 7 publications.

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Light chain type

unknown

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Applications

WB, ELISA

applications

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>Prior to conjugation, the antibodies were analyzed by ELISA.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Prior to conjugation, the antibodies were analyzed by Western Blot.</p>" } } }

style

left-column

Product details

Molar F/P ratio is 3.6:1.

HIS-TAG® is a trademark of EMD Biosciences, Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.1% Sodium azide Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information 6X His tag®

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7225 PubMed40764612

2025

SERBP1-PCIF1 complex-controlled m6Am modification in glutamatergic neurons of the primary somatosensory cortex is required for neuropathic pain in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Huang,Gan Ma,Shan Xie,Runa Wei,Ya Liu,Ying Zeng,Yaxuan Zhao,Qihui Wang,Li Yang,Huiying Huang,Lingyun Hao,Xiaotian Zhao,Hongjun Wang,Wen Shen,Stanley Sau Ching Wong,Jun-Li Cao,Yuan-Xiang Tao,Zhi-Qiang Pan

Cell death & disease 16:496 PubMed40617804

2025

Nedd4-2 ablation in kidney improves glycaemic control in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jantina A Manning,Shilpanjali Jesudason,Paul A B Moretti,Stuart M Pitson,Angela S Y Chou,Meriam Shabbar,Sonia Saad,Carol Pollock,Sharad Kumar

eLife 13: PubMed39665418

2024

Marcks and Marcks-like 1 proteins promote spinal cord development and regeneration in .

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed El Amri,Abhay Pandit,Gerhard Schlosser

Nature protocols 19:3697-3720 PubMed39237830

2024

Mapping protein-DNA interactions with DiMeLo-seq.

Applications

Unspecified application

Species

Unspecified reactive species

Annie Maslan,Nicolas Altemose,Jeremy Marcus,Reet Mishra,Lucy D Brennan,Kousik Sundararajan,Gary Karpen,Aaron F Straight,Aaron Streets

iScience 27:109016 PubMed38327775

2024

DExH-box helicase 9 modulates hippocampal synapses and regulates neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yang,Qiaoqiao Liu,Yaxuan Zhao,Ninghua Lin,Yue Huang,Qihui Wang,Kehui Yang,Runa Wei,Xiaotong Li,Ming Zhang,Lingyun Hao,Hongjun Wang,Zhiqiang Pan

Acta pharmacologica Sinica 44:1748-1767 PubMed37095197

2023

DHX9/DNA-tandem repeat-dependent downregulation of ciRNA-Fmn1 in the dorsal horn is required for neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Dan Liu,Tong Jin,Yang Tao,Ming Zhang,Hong-Li Zheng,Qiao-Qiao Liu,Ke-Hui Yang,Ru-Na Wei,Si-Yuan Li,Yue Huang,Zhou-Ya Xue,Ling-Yun Hao,Qi-Hui Wang,Li Yang,Fu-Qing Lin,Wen Shen,Yuan-Xiang Tao,Hong-Jun Wang,Jun-Li Cao,Zhi-Qiang Pan

Cell 180:323-339.e19 PubMed31928845

2020

Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Del Toro,Maria A Carrasquero-Ordaz,Amy Chu,Tobias Ruff,Meriam Shahin,Verity A Jackson,Matthieu Chavent,Miguel Berbeira-Santana,Goenuel Seyit-Bremer,Sara Brignani,Rainer Kaufmann,Edward Lowe,Rüdiger Klein,Elena Seiradake

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-6X His tag® antibody

Key facts

Host species

Clonality

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Applications

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise