Rabbit Recombinant Monoclonal 6X His tag® antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Transfected cell line - Human samples.
Images
![Flow Cytometry (Intracellular) - FITC Anti-6X His tag® [EPR20547] (AB318311), expandable thumbnail](https://content.abcam.com/products/images/fitc-6x-his-tag-epr20547-ab318311--flow-cytometry-intracellular-img433330.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - FITC Anti-6X His tag® [EPR20547] (AB318311)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
FITC
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | |
|---|---|
| Transfected cell line - Human | Tested |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info 1/500 | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Version
Target data
Alternative names
6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag
Recommended products
Rabbit Recombinant Monoclonal 6X His tag® antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Transfected cell line - Human samples.
Alternative names
6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
FITC
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR20547
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry (Intracellular) - FITC Anti-6X His tag® [EPR20547] (ab318311), expandable thumbnail](https://content.abcam.com/products/images/fitc-6x-his-tag-epr20547-ab318311--flow-cytometry-intracellular-img433330.jpg "Flow Cytometry (Intracellular) - FITC Anti-6X His tag® [EPR20547] (ab318311)")
Flow Cytometry (Intracellular) - FITC Anti-6X His tag® [EPR20547] (ab318311)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with HEK293T-Myc-His-hSNAI1 vector labeling 6X His tag® with ab318311 (middle panel) at 1.0μg/ml (1/500) compared with a Rabbit IgG [EPR25A] FITC (left panel)
Gate is set between transfected and untransfected HEK-293T cells.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com