AB64503

FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker

Name: Anti-alpha Tubulin - Microtubule Marker FITC antibody [DM1A] (ab64503) | Abcam
Brand: Abcam Limited
SKU: AB64503
Price: 405 USD
Availability: InStock
Rating: 5 (6 reviews)

Lab Essentials
What is this?

(6 Reviews)

(25 Publications)

Mouse Monoclonal Alpha-tubulin 4 antibody - conjugated to FITC. Microtubule marker. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 25 publications.

View Alternative Names

TUBA1, TUBA4A, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain

2 Images

Flow Cytometry (Intracellular) - FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB64503)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB64503)

Overlay histogram showing HeLa cells stained with ab64503 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab64503, 0.5μg/1x10⁶ cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 (1μg/1x10⁶ cells ). Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB64503)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB64503)

ICC/IF image of ab64503 stained human HeLa cells. The cells were methanol fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab64503, 1µg/ml, FITC conjugated (green)) for 1h at room temperature. 1% BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker
HRP

HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control
805 Alexa Fluor® 790

Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control
702 Alexa Fluor® 680

Alexa Fluor® 680 Anti-alpha Tubulin antibody [DM1A] - Loading Control
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker
Carrier free

Anti-alpha Tubulin antibody [DM1A] - BSA and Azide free
Unconjugated

Anti-alpha Tubulin antibody [DM1A] - Loading Control

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

DM1A

Isotype

IgG1

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human, Mouse

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

aa426-450.Ab64503 specifically recognizes an epitope in the carboxy-terminal part of alpha-tubulin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "5.1 µL for 10^6 Cells", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-kappa-mopc-21-isotype-control-ab106163'>ab106163</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Gerbil": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Guinea pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Xenopus laevis": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha tubulin also known as DM1A is an important component of the structural unit called microtubules. This protein weighs approximately 50 kDa and acts as a building block in the cytoskeleton. Alpha tubulin is expressed in various cell types across many organisms playing a fundamental role in maintaining cell shape and enabling intracellular transport. Researchers often acetylate alpha tubulin to study post-translational modifications and its effects on microtubule dynamics.

Biological function summary

Alpha tubulin combines with beta tubulin to form the tubulin dimer which is the basic unit of microtubule polymerization. These dimers assemble into microtubule filaments integral in numerous cellular processes. As part of the cytoskeleton network alpha tubulin facilitates roles in mitosis and meiosis providing spindle structures necessary for chromosome separation. Its participation in cell signaling pathways is noteworthy as it interacts with motor proteins like kinesins and dyneins to aid cellular activities.

Pathways

Alpha tubulin holds critical roles in microtubule-associated processes within the cytoskeleton and intracellular transport pathways. It interacts with the MAP (microtubule-associated protein) family which modulates microtubule stability and impacts cell division and trafficking. Moreover the microtubule pathway involves proteins like tau which bind to stabilize microtubules and are implicated in cellular dynamics and signaling.

Alpha tubulin is relevant in neurodegenerative diseases such as Alzheimer's disease and cancer. Aberrant modifications or mutations in alpha tubulin or associated proteins like tau can disrupt microtubule stability contributing to pathological conditions. In cancer altered expression of alpha tubulin affects cell division leading to tumor progression and chemoresistance. Researchers study alpha tubulin using techniques such as immunofluorescence with Alexa fluor 555 to understand its role in disease better.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBA4A

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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:681 PubMed41053117

2025

ALIX mediates reversible gasdermin-D pore formation via the endosomal pathway to limit pyroptosis by active membrane repair.

Applications

Unspecified application

Species

Unspecified reactive species

Sylvia Otchere,Prafulla Shrestha,Himesh N Parmar,Jadyn F Perry,Brittany L Hofmeister,Michelle Steyn,Radhey S Kaushik,Adam D Hoppe,Natalie W Thiex,Ryan L Hanson,Jaime Lopez-Mosqueda,Gergely Imre

Molecular & cellular proteomics : MCP 24:100882 PubMed39571909

2024

Single-Cell Multi-Omics Analysis of In Vitro Post-Ovulatory-Aged Oocytes Revealed Aging-Dependent Protein Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yueshuai Guo,Mengmeng Gao,Xiaofei Liu,Haotian Zhang,Yue Wang,Tong Yan,Bing Wang,Xudong Han,Yaling Qi,Hui Zhu,Chenghao Situ,Yan Li,Xuejiang Guo

Asian journal of andrology 27:113-119 PubMed39254424

2024

A novel homozygous mutation of CFAP300 identified in a Chinese patient with primary ciliary dyskinesia and infertility.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Zhou,Qi Qi,Wen-Hua Wang,Jie Dong,Juan-Juan Xu,Yu-Ming Feng,Zhi-Chuan Zou,Li Chen,Jin-Zhao Ma,Bing Yao

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e23435 PubMed38243686

2024

KAT8 functions in redox homeostasis and mitochondrial dynamics during mouse oocyte meiosis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Wu,Shiwei Wang,Yajun Guo,Shuang Song,Shenming Zeng

Nucleic acids research 51:12224-12241 PubMed37953292

2023

TATDN2 resolution of R-loops is required for survival of BRCA1-mutant cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Aruna S Jaiswal,Arijit Dutta,Gayathri Srinivasan,Yaxia Yuan,Daohong Zhou,Montaser Shaheen,Doraid T Sadideen,Austin Kirby,Elizabeth A Williamson,Yogesh K Gupta,Shaun K Olsen,Mingjiang Xu,Eva Loranc,Pramiti Mukhopadhyay,Alexander Pertsemlidis,Alexander J R Bishop,Patrick Sung,Jac A Nickoloff,Robert Hromas

Nature communications 14:4521 PubMed37607907

2023

TUG1-mediated R-loop resolution at microsatellite loci as a prerequisite for cancer cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Miho M Suzuki,Kenta Iijima,Koichi Ogami,Keiko Shinjo,Yoshiteru Murofushi,Jingqi Xie,Xuebing Wang,Yotaro Kitano,Akira Mamiya,Yuji Kibe,Tatsunori Nishimura,Fumiharu Ohka,Ryuta Saito,Shinya Sato,Junya Kobayashi,Ryoji Yao,Kanjiro Miyata,Kazunori Kataoka,Hiroshi I Suzuki,Yutaka Kondo

Life science alliance 5: PubMed35777956

2022

Multi-omics profiling identifies a deregulated FUS-MAP1B axis in ALS/FTD-associated UBQLN2 mutants.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Strohm,Zehan Hu,Yongwon Suk,Alina Rühmkorf,Erin Sternburg,Vanessa Gattringer,Henrick Riemenschneider,Riccardo Berutti,Elisabeth Graf,Jochen H Weishaupt,Monika S Brill,Angelika B Harbauer,Dorothee Dormann,Jörn Dengjel,Dieter Edbauer,Christian Behrends

Science advances 7:eabg8585 PubMed34797716

2021

Discovery of an F-actin-binding small molecule serving as a fluorescent probe and a scaffold for functional probes.

Applications

Unspecified application

Species

Unspecified reactive species

Takeru Takagi,Tasuku Ueno,Keisuke Ikawa,Daisuke Asanuma,Yusuke Nomura,Shin-Nosuke Uno,Toru Komatsu,Mako Kamiya,Kenjiro Hanaoka,Chika Okimura,Yoshiaki Iwadate,Kenzo Hirose,Tetsuo Nagano,Kaoru Sugimura,Yasuteru Urano

Frontiers in cell and developmental biology 8:592433 PubMed33409275

2020

Imperatorin Ameliorates the Aging-Associated Porcine Oocyte Meiotic Spindle Defects by Reducing Oxidative Stress and Protecting Mitochondrial Function.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Luo,Jia-Bao Zhang,Sheng-Peng Li,Wen Liu,Xue-Rui Yao,Hao Guo,Zhe-Long Jin,Yong-Xun Jin,Bao Yuan,Hao Jiang,Nam-Hyung Kim

Journal of cell science 133: PubMed32843575

2020

ACSL3 is a novel GABARAPL2 interactor that links ufmylation and lipid droplet biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Franziska Eck,Santosh Phuyal,Matthew D Smith,Manuel Kaulich,Simon Wilkinson,Hesso Farhan,Christian Behrends

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker

Flow Cytometry (Intracellular) - FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB64503)

Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB64503)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (25)

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