JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB27637

FITC Anti-Apolipoprotein B antibody

Be the first to review this product! Submit a review

|

(6 Publications)

Goat Polyclonal Apolipoprotein B antibody - conjugated to FITC. Suitable for ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human APOB.

View Alternative Names

Apolipoprotein B-100, Apo B-100, APOB, Apolipoprotein B100

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Native Full Length Protein corresponding to Human APOB.

P04114

Specificity

This antibody is specific for human apo B-100 (apolipoprotein B).

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Molar F/P ratio is 6.1.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified by human apo B-100-Sepharose™ affinity column.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 0.5% BSA, 0.435% Sodium chloride, 0.0146% EDTA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein B often referred to as ApoB is a core protein component of low-density lipoproteins (LDL) and is essential for their assembly and secretion. ApoB is also known as apolipoproteine B and apolipoprotein B protein. This protein has a large molecular weight approximately 550 kDa. ApoB is primarily expressed in the liver and to some extent in the intestine. Its large size allows it to provide structural integrity to lipoprotein particles playing a role in lipid metabolism.
Biological function summary

ApoB plays a significant role in lipid transport and metabolism. It is a critical component of the lipoprotein particle complex which is responsible for the transport of lipids such as cholesterol and triglycerides through the bloodstream. ApoB helps in the binding of lipoproteins to cell receptors facilitating the uptake of lipids by tissues. Its presence on the surface of lipoproteins makes it important for normal cholesterol homeostasis in the human body.

Pathways

ApoB is integrated within the lipid metabolism pathway. It interacts with proteins such as microsomal triglyceride transfer protein (MTP) during the assembly and secretion of lipoproteins. This pathway is essential for the normal transport of lipids throughout the body and influences processes like absorption of dietary fats. ApoB also plays a role in the pathways related to atherosclerosis where it influences the deposition of cholesterol in arterial walls.

ApoB is well-associated with cardiovascular diseases notably atherosclerosis and familial hypercholesterolemia. In these conditions elevated levels of ApoB correlate to an increased risk of plaque formation in blood vessels. ApoB in conjunction with LDL receptor and other related proteins influences lipid homeostasis and disorders where lipid oxidation or accumulation is present. Monitoring ApoB and its concentration can be vital for assessing cardiovascular disease risk.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
See full target information APOB
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 13:e70008 PubMed39441012

2024

Separation of small extracellular vesicles (sEV) from human blood by Superose 6 size exclusion chromatography.

Applications

Unspecified application

Species

Unspecified reactive species

Jerome Nouvel,Gonzalo Bustos-Quevedo,Tony Prinz,Ramsha Masood,George Daaboul,Tanja Gainey-Schleicher,Uwe Wittel,Sophia Chikhladze,Bence Melykuti,Martin Helmstaedter,Karl Winkler,Irina Nazarenko,Gerhard Pütz

Cancers 14: PubMed35565197

2022

Efficient Small Extracellular Vesicles (EV) Isolation Method and Evaluation of EV-Associated DNA Role in Cell-Cell Communication in Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Venkatesh Kumar Chetty,Jamal Ghanam,Srishti Anchan,Katarina Reinhardt,Alexandra Brenzel,Márton Gelléri,Christoph Cremer,Elena Grueso-Navarro,Markus Schneider,Nils von Neuhoff,Dirk Reinhardt,Jadwiga Jablonska,Irina Nazarenko,Basant Kumar Thakur

Frontiers in bioengineering and biotechnology 9:794676 PubMed34926432

2021

LDL-Based Lipid Nanoparticle Derived for Blood Plasma Accumulates Preferentially in Atherosclerotic Plaque.

Applications

Unspecified application

Species

Unspecified reactive species

Christian A Boada,Assaf Zinger,Scott Rohen,Jonathan O Martinez,Michael Evangelopoulos,Roberto Molinaro,Madeleine Lu,Ramiro Alejandro Villarreal-Leal,Federica Giordano,Manuela Sushnitha,Enrica De Rosa,Jens B Simonsen,Sergey Shevkoplyas,Francesca Taraballi,Ennio Tasciotti

International journal of nanomedicine 15:10241-10256 PubMed33364756

2020

Quality Assessment and Comparison of Plasma-Derived Extracellular Vesicles Separated by Three Commercial Kits for Prostate Cancer Diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

Bairen Pang,Ying Zhu,Jie Ni,Juanfang Ruan,James Thompson,David Malouf,Joseph Bucci,Peter Graham,Yong Li

Science advances 6: PubMed33219024

2020

High-throughput single-EV liquid biopsy: Rapid, simultaneous, and multiplexed detection of nucleic acids, proteins, and their combinations.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zhou,Zuoren Wu,Jie Hu,Dawei Yang,Xiaoyan Chen,Qin Wang,Jie Liu,Maosen Dou,Wenjun Peng,Yuanyuan Wu,Wenhao Wang,Chenjian Xie,Ming Wang,Yuanlin Song,Hengshan Zeng,Chunxue Bai

Journal of lipid research 52:2323-31 PubMed21971713

2011

Multidimensional profiling of plasma lipoproteins by size exclusion chromatography followed by reverse-phase protein arrays.

Applications

Unspecified application

Species

Unspecified reactive species

Gregor Dernick,Stefan Obermüller,Cyrill Mangold,Christine Magg,Hugues Matile,Oliver Gutmann,Elisabeth von der Mark,Corinne Handschin,Cyrille Maugeais,Eric J Niesor
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB27622

HRP Anti-Apolipoprotein B antibody

  • 0
  • 0
Primary Antibodies

AB31992

Anti-Apolipoprotein B antibody

  • 4
  • 3
Primary Antibodies

AB39560

Anti-Apolipoprotein B antibody [7B8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein B antibody [7B8] (AB39560)

  • 0
  • 0
Primary Antibodies

AB7616

Anti-Apolipoprotein B antibody

Western blot - Anti-Apolipoprotein B antibody (AB7616)

  • 4
  • 2
Primary Antibodies

AB20737

Anti-Apolipoprotein B antibody

  • 4
  • 6
Primary Antibodies

AB239581

Anti-Apolipoprotein B antibody [C3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein B antibody [C3] (AB239581)

  • 0
  • 0
Primary Antibodies

AB231574

Anti-Apolipoprotein B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein B antibody (AB231574)

  • 0
  • 0
Primary Antibodies

AB139401

Anti-Apolipoprotein B antibody [EPR2914]

RabMAb|Recombinant

Western blot - Anti-Apolipoprotein B antibody [EPR2914] (AB139401)

  • 0
  • 0
Primary Antibodies

AB231683

Anti-Apolipoprotein B antibody [EPR2914] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Apolipoprotein B antibody [EPR2914] - BSA and Azide free (AB231683)

  • 0
  • 0

Complementary Products

Assay Kits

AB65390

Cholesterol Assay Kit - HDL and LDL/VLDL

Biochemical assay - Cholesterol Assay Kit - HDL and LDL/VLDL (AB65390)

  • 4
  • 10
Primary Antibodies

AB208184

Anti-Lipoprotein a antibody [EPR19731]

RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein a antibody [EPR19731] (AB208184)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB280330

Recombinant human Apolipoprotein E3

Mass Spectrometry - Recombinant human Apolipoprotein E3 (AB280330)

  • 0
  • 0
Primary Antibodies

AB282174

PE Anti-Apolipoprotein A I antibody [EP1368Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-Apolipoprotein A I antibody [EP1368Y] (AB282174)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com