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Rabbit Recombinant Monoclonal Aurora A phospho T288 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human AURKB phospho T232.

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Images

Flow Cytometry - FITC Anti-Aurora B (phospho T232) + Aurora A (phospho T288) + IPL-1/STK13/Aurora C (phospho T198) antibody [AuroraABC-CC12] (AB278776), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 99.71% PBS, 0.2% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human AURKB phospho T232. The exact immunogen used to generate this antibody is proprietary information. Database link Q96GD4

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species
Human
Dilution info
5 µL for 106 Cells
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

Function

Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression (PubMed:11039908, PubMed:12390251, PubMed:17125279, PubMed:17360485, PubMed:18615013, PubMed:26246606). Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis (PubMed:14523000, PubMed:26246606). Required for normal spindle positioning during mitosis and for the localization of NUMA1 and DCTN1 to the cell cortex during metaphase (PubMed:27335426). Required for initial activation of CDK1 at centrosomes (PubMed:13678582, PubMed:15128871). Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2 (PubMed:11551964, PubMed:14702041, PubMed:15128871, PubMed:15147269, PubMed:15987997, PubMed:17604723, PubMed:18056443, PubMed:18615013). Regulates KIF2A tubulin depolymerase activity (PubMed:19351716). Important for microtubule formation and/or stabilization (PubMed:18056443). Required for normal axon formation (PubMed:19812038). Plays a role in microtubule remodeling during neurite extension (PubMed:19668197). Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and destabilizing p53/TP53 (PubMed:14702041). Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity (PubMed:11551964). Inhibits cilia outgrowth (By similarity). Required for cilia disassembly via phosphorylation of HDAC6 and subsequent deacetylation of alpha-tubulin (PubMed:17604723, PubMed:20643351). Regulates protein levels of the anti-apoptosis protein BIRC5 by suppressing the expression of the SCF(FBXL7) E3 ubiquitin-protein ligase substrate adapter FBXL7 through the phosphorylation of the transcription factor FOXP1 (PubMed:28218735).

Additional Targets

AURKB phospho T232, AURKC phospho T198, AURKB phospho T232, AURKC phospho T198

Alternative names

AIK, AIRK1, ARK1, AURA, AYK1, BTAK, IAK1, STK15, STK6, AURKA, Aurora kinase A, Aurora 2, Aurora/IPL1-related kinase 1, Breast tumor-amplified kinase, Ipl1- and aurora-related kinase 1, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase Ayk1, Serine/threonine-protein kinase aurora-A, ARK-1, Aurora-related kinase 1

Recommended products

Rabbit Recombinant Monoclonal Aurora A phospho T288 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human AURKB phospho T232.

Key facts

Isotype
IgG
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogens
  • Synthetic Peptide within Human AURKB phospho T232. The exact immunogen used to generate this antibody is proprietary information. Database link Q96GD4
Clone number
AuroraABC-CC12
Purification technique
Affinity purification Protein A/G
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Aurora kinases are a family of serine/threonine kinases including Aurora A Aurora B and Aurora C which play key roles in mitotic regulation. Aurora A primarily functions in centrosome maturation and separation spindle assembly and ensuring accurate chromosomal segregation often located at spindle poles. Aurora B is integral to chromosome condensation spindle checkpoint and cytokinesis positioning itself at the centromere and later moving to the central spindle and midbody. Aurora C shares functions with Aurora B and is mainly found in testicular tissue. Aurora A and B have molecular masses around 46-48 kDa and Aurora C is slightly smaller. These kinases express in proliferative tissues with Aurora C found largely in the testes.

Biological function summary

Aurora kinases are necessary for accurate cell division. Aurora A contributes to centrosome duplication and mitotic entry forming a complex with other proteins like TPX2 to regulate microtubule dynamics. Aurora B a critical component of the chromosomal passenger complex includes partner proteins such as INCENP Survivin and Borealin. This complex controls the correct biorientation of chromosomes and execution of cytokinesis. Aurora C although less studied is known to function in meiotic cell division in germ cells often compensating for Aurora B's functions.

Pathways

Aurora kinases play important roles in the regulation of the cell cycle. Aurora A is part of the mitotic spindle checkpoint pathway and coordinates with other proteins such as PLK1 and CDC25B to ensure proper cell cycle progression. Aurora B is involved in the spindle assembly checkpoint interacting with proteins like BubR1 and Mad2 to monitor and correct kinetochore-microtubule attachments. These pathways ensure cells do not divide with errors preventing aneuploidy and maintaining genomic stability.

Associated diseases and disorders

The dysregulation of Aurora kinases associates with cancer particularly due to their role in cell division. Aberrant expression or activation of Aurora A is linked to the development of several cancers including breast cancer connecting through proteins like p53 which can become dysfunctional. Aurora B overexpression also relates to malignancies such as colorectal cancer often working in conjunction with oncoproteins such as MYC. The correlation of these kinases with tumorigenesis makes them significant targets for cancer therapy research.

Product promise

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1 product image

  • Flow Cytometry - FITC Anti-Aurora B (phospho T232) + Aurora A (phospho T288) + IPL-1/STK13/Aurora C (phospho T198) antibody [AuroraABC-CC12] (ab278776), expandable thumbnail

    Flow Cytometry - FITC Anti-Aurora B (phospho T232) + Aurora A (phospho T288) + IPL-1/STK13/Aurora C (phospho T198) antibody [AuroraABC-CC12] (ab278776)

    Flow cytometric analysis of HeLa cells untreated and unstained as negative control (blue) or untreated (red) or treated with nocodazole (green) and stained using ab278776.

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Product protocols

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