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Rabbit Recombinant Monoclonal BACE1 antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human BACE1 aa 1-500.

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Images

Flow Cytometry (Intracellular) - FITC Anti-BACE1 antibody [027] (AB275650), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 0.5% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human BACE1 aa 1-500. The exact immunogen used to generate this antibody is proprietary information. Database link P56817

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)
Human
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

10 μl/Test

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase (PubMed:10656250, PubMed:10677483, PubMed:20354142). Cleaves CHL1 (By similarity).

Alternative names

BACE, KIAA1149, BACE1, Beta-secretase 1, Aspartyl protease 2, Beta-site amyloid precursor protein cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2, ASP2, Asp 2, Beta-site APP cleaving enzyme 1

Recommended products

Rabbit Recombinant Monoclonal BACE1 antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human BACE1 aa 1-500.

Key facts

Isotype
IgG
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human BACE1 aa 1-500. The exact immunogen used to generate this antibody is proprietary information. Database link P56817
Clone number
027
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze, Store in the dark

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

BACE1 also known as beta-site APP cleaving enzyme 1 or beta-secretase plays an important role in the cleavage of amyloid precursor protein (APP). This cleavage results in the production of amyloid-beta peptides which are associated with Alzheimer's disease. BACE1 is a membrane-bound aspartic protease and has a molecular weight of approximately 50100 Da. The enzyme expresses mostly in the brain's neurons and some secretory tissues.

Biological function summary

BACE1 initiates the amyloidogenic pathway of APP processing which involves amyloid-beta generation. BACE1 doesn't function alone but acts as part of a complex that aids in protein substrate recognition and processing. Its activity contributes to physiological processes like myelination and axonal guidance indicating its importance beyond amyloid production.

Pathways

BACE1 is integral to the amyloidogenic pathway which is important in Alzheimer's disease development. It interacts with proteins such as presenilin 1 a part of the gamma-secretase complex that further processes the amyloid-beta precursor. Furthermore BACE1 links to synaptic functions and neural signaling pathways highlighting its multifaceted roles.

Associated diseases and disorders

BACE1 holds significance in Alzheimer's disease due to its role in amyloid-beta peptide production. This connection has led to the development of BACE1 inhibitors as potential therapeutic agents. Additionally BACE1’s involvement in other neural functions ties it to cognitive impairments. It also relates to APP through its function in Alzheimer's suggesting targeted strategies for treatment could involve modulating BACE1 activity.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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1 product image

  • Flow Cytometry (Intracellular) - FITC Anti-BACE1 antibody [027] (ab275650), expandable thumbnail

    Flow Cytometry (Intracellular) - FITC Anti-BACE1 antibody [027] (ab275650)

    Intracellular flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling BACE1 with ab275650 (Balck) compared with isotype control (Grey). The cells were treated according to manufacturers manual. The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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