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AB139543

FITC Anti-Bax antibody [T22-A]

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(4 Publications)

Rabbit Monoclonal BAX antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human BAX.

View Alternative Names

BCL2L4, BAX, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4

1 Images
Flow Cytometry - FITC Anti-Bax antibody [T22-A] (AB139543)
  • Flow Cyt

Unknown

Flow Cytometry - FITC Anti-Bax antibody [T22-A] (AB139543)

Flow cytometry analysis of Human HEK293 cell line, staining Bax with ab139543. Cells were fixed, permeabilized and stained with FITC-conjugated ab139543 (blue, 10 μl per test) or with an isotype control (black).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

T22-A

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human BAX. The exact immunogen used to generate this antibody is proprietary information.

Q07812

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/100", "FlowCyt-species-notes": "<p>Use 1×10<sup>6</sup> cells per 100 µl</p>" } } }
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Product details

The antibody is conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
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Properties and storage information

Form
Liquid
Purification technique
Proprietary technique
Purification notes
This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a propriety technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bax also known as Bcl-2-associated X protein plays an important role in the regulation of apoptosis. It is widely known for its pro-apoptotic function opposing the action of anti-apoptotic proteins like Bcl-2. Bax has a molecular weight of approximately 21 kDa making it identifiable in laboratory techniques such as western blot. Researchers often use anti-Bax antibodies to detect and analyze its expression levels in various studies. The Bax protein is mainly expressed in mitochondria where it undergoes changes in response to apoptotic signals.
Biological function summary

The Bax protein forms homodimers or heterodimers with other Bcl-2 family proteins to facilitate apoptosis. This protein is part of a larger complex involving several members of the Bcl-2 family which finely tunes the balance between cell survival and programmed cell death. Through a change in its conformation Bax translocates to the outer mitochondrial membrane where it promotes the release of cytochrome c and other apoptogenic factors into the cytosol.

Pathways

Bax plays a significant role in the intrinsic pathway of apoptosis also known as the mitochondrial pathway. This pathway involves the release of cytochrome c which further activates downstream proteins such as caspase-9 and caspase-3. Bax interacts closely with proteins like Bak another pro-apoptotic member of the Bcl-2 family providing a cooperative function in mitochondrial membrane permeabilization. Together Bax and Bak act as gatekeepers of the mitochondrial pathway determining the cell's fate in response to apoptotic stimuli.

The dysregulation of Bax often associates with various cancers and neurodegenerative diseases. In cancer reduced Bax expression or function can lead to resistance to apoptosis contributing to unchecked cellular proliferation. Conversely in neurodegenerative disorders such as Alzheimer's disease increased Bax activity results in excessive neuronal apoptosis. The balance between Bax and its partner proteins like Bcl-2 or Bcl-xL is important in maintaining normal cellular function and preventing disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the mitochondrial apoptotic process (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 36361894, PubMed : 8358790, PubMed : 8521816). Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed : 21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816). Promotes activation of CASP3, and thereby apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816).
See full target information BAX
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0321022 PubMed40184394

2025

Restorative effects of Momordica charantia extract on cerebellar GFAP and NGF expression in pregnant diabetic rats and their offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Amoura M Abou-El-Naga,Hend Abd El-Halim Mansour,Mamdouh R El-Sawi,Mai Alaa El-Dein,Yasmin M Tag,Reham A Ghanem,Manar A Shawki

Journal of enzyme inhibition and medicinal chemistry 39:2343352 PubMed38700244

2024

The activity of pyrazolo[4,3-][1,2,4]triazine and pyrazolo[4,3-]tetrazolo[1,5-][1,2,4]triazine sulphonamide derivatives in monolayer and spheroid breast cancer cell cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Szymanowska,Dominika Radomska,Robert Czarnomysy,Mariusz Mojzych,Katarzyna Kotwica-Mojzych,Krzysztof Bielawski,Anna Bielawska

Antioxidants (Basel, Switzerland) 12: PubMed36978864

2023

Differential Apoptotic Effects of Bee Product Mixtures on Normal and Cancer Hepatic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Vanesa Sánchez-Martín,Paloma Morales,Amaia Iriondo-DeHond,Xavier F Hospital,Manuela Fernández,Eva Hierro,Ana I Haza

Animal bioscience 35:1010-1020 PubMed34530507

2021

Effects of maternal undernutrition during late pregnancy on the regulatory factors involved in growth and development in ovine fetal perirenal brown adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Yang,Chi Ma,Yang Zi,Min Zhang,Yingchun Liu,Kaifeng Wu,Feng Gao
View all publications
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Product promise

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