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AB272337

FITC Anti-Bcl-2 antibody [Bcl-2/100]

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Mouse Monoclonal Bcl-2 antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human BCL2 aa 1-100.

View Alternative Names

Apoptosis regulator Bcl-2, BCL2

2 Images
Flow Cytometry (Intracellular) - FITC Anti-Bcl-2 antibody [Bcl-2/100] (AB272337)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-Bcl-2 antibody [Bcl-2/100] (AB272337)

Flow cytometry intracellular staining pattern of human peripheral whole blood stained usingab272337 at 4 μl reagent / 100 μl of peripheral whole blood.

Flow Cytometry (Intracellular) - FITC Anti-Bcl-2 antibody [Bcl-2/100] (AB272337)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-Bcl-2 antibody [Bcl-2/100] (AB272337)

Separation of human Bcl-2 positive lymphocytes (red-filled) from blood debris (black-dashed) in Intracellular Flow Cytometry analysis (intracellular staining) of human peripheral whole blood stained using ab272337 at 4 μl reagent / 100 μl of peripheral whole blood.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Bcl-2/100

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human BCL2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P10415

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Reactivity data

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AB119506

Human Bcl-2 ELISA Kit

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified from TCS.
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.
Biological function summary

The role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.

Pathways

Bcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.

The dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed : 1508712, PubMed : 8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed : 11368354). Appears to function in a feedback loop system with caspases (PubMed : 11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed : 11368354). Also acts as an inhibitor of autophagy : interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed : 18570871, PubMed : 20889974, PubMed : 21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed : 17418785).
See full target information BCL2
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Product promise

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