AB6277

FITC Anti-beta Actin antibody [AC-15] - Cytoskeleton Marker

Name: Anti-beta Actin FITC antibody [AC-15] (ab6277) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB6277
Price: 510 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(26 Publications)

Mouse Monoclonal beta Actin antibody - conjugated to FITC. Suitable for ICC/IF and reacts with Rat, Chicken samples. Cited in 26 publications. Immunogen corresponding to Synthetic Peptide within Human ACTB aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Beta-actin, ACTB

3 Images

Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] - Cytoskeleton Marker (AB6277)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] - Cytoskeleton Marker (AB6277)

ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.

30 min blocking with TBS-T / 5% BSA
1 hr incubation with ab6277 (TBS-T / 5% BSA)
1 minute nuclear counterstaining with DAPI

Magnification x40 no deconvolution.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] - Cytoskeleton Marker (AB6277)

ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.

30 min blocking with TBS-T / 5% BSA
1 hr incubation with ab6277 (TBS-T / 5% BSA)
1 minute nuclear counterstaining with DAPI

Magnification x20

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] - Cytoskeleton Marker (AB6277)

Immunocytochemistry/ Immunofluorescence analysis of chicken fibroblast cells labeling beta Actin with ab6277 at 1/300 dilution. The cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 1/300 Anti-beta Actin antibody [AC-15] (FITC) (ab6277).

Unconjugated

Anti-beta Actin antibody [AC-15] - Loading Control
HRP

HRP Anti-beta Actin antibody [AC-15] - Loading Control

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AC-15

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Rat, Chicken

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human ACTB aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P60709

Epitope

The N-terminal of the beta isoform of actin

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Chicken": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Immunofluoresence staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membrane associated dense-plaque.

Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

Purified Immunoglobulin

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.

Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed : 25255767, PubMed : 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed : 29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed : 29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

See full target information ACTB

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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40724960

2025

Citrate Transporter Expression and Localization: The Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Jan C-C Hu,Tian Liang,Hong Zhang,Yuanyuan Hu,Yasuo Yamakoshi,Ryuji Yamamoto,Chuhua Zhang,Hui Li,Charles E Smith,James P Simmer

Scientific reports 15:24913 PubMed40640300

2025

DSP and DPP are dispensable for initiation of dentin and enamel mineralization but critical for circumpulpal dentin mineralization.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Liang,Charles E Smith,Yuanyuan Hu,Hong Zhang,Chuhua Zhang,James P Simmer,Jan C-C Hu

Cytoskeleton (Hoboken, N.J.) 82:302-310 PubMed39305224

2024

F-actin in the cuticular plate and junctions of auditory hair cells is regulated by ADF and cofilin to allow for normal stereocilia bundle patterning and maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Jamis McGrath,Katelin Hawbaker,Benjamin J Perrin

iScience 26:108495 PubMed38089592

2023

Hypoxia induces hepatocellular carcinoma metastasis via the HIF-1α/METTL16/lnc-CSMD1-7/RBFOX2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yingchao Wang,Yong Yang,Ye Yang,Yuan Dang,Zhiting Guo,Qiuyu Zhuang,Xiaoyuan Zheng,Fei Wang,Niangmei Cheng,Xiaolong Liu,Wuhua Guo,Bixing Zhao

eLife 12: PubMed37982489

2023

Differential regulation of hair cell actin cytoskeleton mediated by SRF and MRTFB.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Yun Zhou,Chen-Xi Jin,Wen-Xiao Wang,Lei Song,Jung-Bum Shin,Ting-Ting Du,Hao Wu

Scientific reports 12:16477 PubMed36183038

2022

Enamel defects in Acp4 mice and human ACP4 mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Liang,Shih-Kai Wang,Charles Smith,Hong Zhang,Yuanyuan Hu,Figen Seymen,Mine Koruyucu,Yelda Kasimoglu,Jung-Wook Kim,Chuhua Zhang,Thomas L Saunders,James P Simmer,Jan C-C Hu

Journal of cell science 135: PubMed35022745

2022

Label2label: training a neural network to selectively restore cellular structures in fluorescence microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Sophie Kölln,Omar Salem,Jessica Valli,Carsten Gram Hansen,Gail McConnell

Current biology : CB 31:1141-1153.e7 PubMed33400922

2021

Actin at stereocilia tips is regulated by mechanotransduction and ADF/cofilin.

Applications

Unspecified application

Species

Unspecified reactive species

Jamis McGrath,Chun-Yu Tung,Xiayi Liao,Inna A Belyantseva,Pallabi Roy,Oisorjo Chakraborty,Jinan Li,Nicolas F Berbari,Christian C Faaborg-Andersen,Melanie Barzik,Jonathan E Bird,Bo Zhao,Lata Balakrishnan,Thomas B Friedman,Benjamin J Perrin

PloS one 14:e0216251 PubMed31039189

2019

A 61% lighter cell culture dish to reduce plastic waste.

Applications

Unspecified application

Species

Unspecified reactive species

Pedro Réu,Gustav Svedberg,Lars Hässler,Björn Möller,Helene Andersson Svahn,Jesper Gantelius

Molecular cell 73:474-489.e5 PubMed30595434

2019

Noncanonical Modulation of the eIF2 Pathway Controls an Increase in Local Translation during Neural Wiring.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Cagnetta,Hovy Ho-Wai Wong,Christian K Frese,Giovanna R Mallucci,Jeroen Krijgsveld,Christine E Holt

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-beta Actin antibody [AC-15] - Cytoskeleton Marker

Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] - Cytoskeleton Marker (AB6277)

Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] - Cytoskeleton Marker (AB6277)

Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] - Cytoskeleton Marker (AB6277)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (26)

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