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Mouse Monoclonal beta Actin antibody - conjugated to FITC. Suitable for ICC/IF and reacts with Rat, Chicken samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Human ACTB aa 1-50 conjugated to Keyhole Limpet Haemocyanin.


Images

Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (AB6277), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (AB6277), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (AB6277), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human ACTB aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P60709

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Rat
Tested
Chicken
Tested

Tested
Tested

Species
Rat, Chicken
Dilution info
-
Notes

-

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Select an associated product type

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1 product for Alternative Version

Target data

Function

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

Alternative names

Beta-actin, ACTB

Recommended products

Mouse Monoclonal beta Actin antibody - conjugated to FITC. Suitable for ICC/IF and reacts with Rat, Chicken samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Human ACTB aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

Key facts

Isotype
IgG1
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human ACTB aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P60709
Clone number
AC-15
Purity
IgG fraction
Epitope
The N-terminal of the beta isoform of actin
Concentration
Loading...
Purification notes

Purified Immunoglobulin

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Immunofluoresence staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membrane associated dense-plaque.

Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Beta actin also known as beta cytoplasmic actin plays a central role in cell structure and motility. It is part of the actin protein family and is widely expressed in eukaryotic cells. The molecular weight of beta actin is approximately 42 kDa. It contributes to the formation of the cytoskeleton and participates in various cellular processes including movement and stability. Actin is abundant in all cell types providing structural integrity and flexibility.

Biological function summary

Beta actin contributes to the maintenance of cell shape and is an important player in cell division and muscle contraction. It forms part of a larger actin filaments network often associating with other proteins to form the actin cytoskeleton complex. This complex supports cellular processes such as signaling intracellular trafficking and positioning of organelles. The dynamic polymerization and depolymerization of actin filaments are critical for cellular functions.

Pathways

Beta actin functions in the regulation of important biological pathways such as the Rho/Rac/Cdc42 signaling pathway and the Wnt signaling pathway. These pathways are essential in numerous cellular activities including cell morphology and gene transcription. Beta actin closely interacts with proteins like myosin and tropomyosin which facilitate its role in muscle contraction and cell division and proteins such as Rac and Cdc42 which help govern cytoskeletal dynamics and cellular responses to extracellular stimuli.

Associated diseases and disorders

Beta actin has links to cancers and muscular dystrophies. Aberrations in actin dynamics can result in tumor cell migration and metastasis making it a component of interest in cancer research. Additionally mutations or dysregulation in actin-associated proteins may contribute to muscular dystrophies affecting muscle function and strength. Beta actin's interactions with proteins like dystrophin involved in maintaining muscle integrity further highlight its relevance in both biological functions and disease contexts.

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3 product images

  • Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)

    ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.

    30 min blocking with TBS-T / 5% BSA
    1 hr incubation with ab6277 (TBS-T / 5% BSA)
    1 minute nuclear counterstaining with DAPI

    Magnification x20

  • Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)

    Immunocytochemistry/ Immunofluorescence analysis of chicken fibroblast cells labeling beta Actin with ab6277 at 1/300 dilution. The cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 1/300 Anti-beta Actin antibody [AC-15] (FITC) (ab6277).

  • Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - FITC Anti-beta Actin antibody [AC-15] (ab6277)

    ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.

    30 min blocking with TBS-T / 5% BSA
    1 hr incubation with ab6277 (TBS-T / 5% BSA)
    1 minute nuclear counterstaining with DAPI

    Magnification x40 no deconvolution.

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