AB25770

FITC Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker

Name: FITC Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (ab25770) | Abcam
Brand: Abcam Limited
SKU: AB25770
Price: 545 USD
Availability: InStock

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(8 Publications)

Mouse Monoclonal Beta-3-tubulin antibody - conjugated to FITC. Suitable for ICC, IHC-Fr and reacts with Mouse samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human TUBB3 aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

TUBB4, TUBB3, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III

1 Images

Supplier Data

Immunocytochemistry - FITC Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (AB25770)

Immunocystochemistry analysis of mouse Neuro2a cell line staining beta III Tubulin with ab25770 at 1 μg/ ml concentration (green), and nuclear staining with DAPI (blue).

Unconjugated

Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

TU-20

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse

Applications

IHC-Fr, ICC

applications

Immunogen

Synthetic Peptide within Human TUBB3 aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q13509

Specificity

This antibody recognizes the peptide sequence ESESQGPK (amino acids 441-448) of human class III beta tubulin specific for neurones.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1 µg/mL", "ICC-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }

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Product details

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Purity >95% by SDS-PAGE.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta III tubulin often referred to as βIII tubulin or beta-tubulin 3 is a microtubule element involved in the cellular cytoskeleton structure. This protein with a molecular mass of approximately 55 kDa plays an important role in the development and maintenance of neuron structures. It is most prominently expressed in neurons within the central and peripheral nervous systems. In these cells beta III tubulin contributes to microtubule polymerization facilitating the formation of the intricate network necessary for cell shape intracellular transport and division.

Biological function summary

Beta III tubulin acts in stabilizing microtubules which are essential for proper neuronal function. It exists as part of the tubulin dimer complex partnering with alpha-tubulin to form the building blocks of microtubules. This particular isotype is often used as a marker for neuronal differentiation due to its specific expression pattern in neural tissues. Its regulation and expression levels are important for neurogenesis and maintaining neuronal plasticity.

Pathways

Beta III tubulin plays a role in neuron-specific intracellular transport and signaling pathways like the Rho GTPase and MAPK signaling pathways. These pathways are involved in cell cycle regulation and apoptotic processes. Relationships with other proteins such as kinesins and dyneins are important in these pathways influencing intracellular transport and signaling through binding and moving along the microtubule tracks created by beta-tubulin isotypes including beta III tubulin.

Abnormal beta III tubulin expression has been associated with cancer particularly in tumors originating from neuronal lineage such as glioblastomas. Overexpression or mutations can contribute to chemoresistance complicating treatment for certain types of cancer. Beta III tubulin is also linked to neurodevelopmental disorders as it affects proper neural network formation and stability. Its relationship with tumor protein p53 is noted in cancer pathways as p53 can influence beta III tubulin expression impacting cellular proliferation and apoptosis in oncogenic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers (PubMed : 34996871, PubMed : 38305685, PubMed : 38609661). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed : 34996871, PubMed : 38305685, PubMed : 38609661). Below the cap, alpha-beta tubulin heterodimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed : 34996871, PubMed : 38609661). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed : 20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed : 28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed : 28483977).

See full target information TUBB3

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 30:85-97 PubMed37575879

2023

Bionic peptide scaffold in situ polarization and recruitment of M2 macrophages to promote peripheral nerve regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Pengxiang Yang,Yong Peng,Xiu Dai,Jing Jie,Deling Kong,Xiaosong Gu,Yumin Yang

Bioengineered 12:6935-6951 PubMed34559584

2021

Alteration of long non-coding RNAs and mRNAs expression profiles by compound heterozygous ASXL3 mutations in the mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Songhui Zhang,Fang Fu,Li Zhen,Ru Li,Can Liao

Journal of biomedical science 24:78 PubMed29037191

2017

Ascorbic acid alters cell fate commitment of human neural progenitors in a WNT/β-catenin/ROS signaling dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Tareck Rharass,Margareta Lantow,Adam Gbankoto,Dieter G Weiss,Daniela Panáková,Stéphanie Lucas

The Journal of biological chemistry 289:27937-51 PubMed25124032

2014

Ca2+-mediated mitochondrial reactive oxygen species metabolism augments Wnt/β-catenin pathway activation to facilitate cell differentiation.

Applications

ICC

Species

Human

Tareck Rharass,Heiko Lemcke,Margareta Lantow,Sergei A Kuznetsov,Dieter G Weiss,Daniela Panáková

Tissue engineering. Part A 20:1603-11 PubMed24328537

2014

Bioengineering of physiologically functional intrinsically innervated human internal anal sphincter constructs.

Applications

Unspecified application

Species

Unspecified reactive species

Robert R Gilmont,Shreya Raghavan,Sita Somara,Khalil N Bitar

The European journal of neuroscience 38:2218-28 PubMed23607708

2013

Neuronal differentiation requires a biphasic modulation of gap junctional intercellular communication caused by dynamic changes of connexin43 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Heiko Lemcke,Marie-Louise Nittel,Dieter G Weiss,Sergei A Kuznetsov

Gastroenterology 141:310-9 PubMed21463628

2011

Successful implantation of bioengineered, intrinsically innervated, human internal anal sphincter.

Applications

IHC-Fr

Species

Mouse

Shreya Raghavan,Robert R Gilmont,Eiichi A Miyasaka,Sita Somara,Shanthi Srinivasan,Daniel H Teitelbaum,Khalil N Bitar

Cell death and differentiation 15:1408-16 PubMed18551132

2008

HtrA1-dependent proteolysis of TGF-beta controls both neuronal maturation and developmental survival.

Applications

IHC-FoFr

Species

Mouse

S Launay,E Maubert,N Lebeurrier,A Tennstaedt,M Campioni,F Docagne,C Gabriel,L Dauphinot,M C Potier,M Ehrmann,A Baldi,D Vivien

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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FITC Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker

Immunocytochemistry - FITC Anti-beta III Tubulin antibody [TU-20] - Neuronal Marker (AB25770)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise